################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AatII 2825 GACGTC GACGT^C AccI 2434 GTCTAC GT^[AC][GT]AC AflIII 2430 ACACGT A^C[GA][CT]GT AseI 1804 ATTAAT AT^TAAT AsnI 1804 ATTAAT AT^TAAT AvaI 132 CCCGAG C^[CT]CG[GA]G BamHI 1956 GGATCC G^GATCC BsaAI 655 TACGTG [CT]AC^GT[GA] BsiWI 892 CGTACG C^GTACG BsoBI 132 CCCGAG C^[CT]CG[GA]G BspMI 612 ACCTGC ACCTGC(4/8) BssSI 1334 CTCGTG CACGAG(-5/-1) DrdI 1678 GACAGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1687 GTCAAC GT[CT]^[GA]AC HindII 1687 GTCAAC GT[CT]^[GA]AC MfeI 291 CAATTG C^AATTG MunI 291 CAATTG C^AATTG NaeI 1730 GCCGGC GCC^GGC NcoI 2855 CCATGG C^CATGG NgoMIV 1730 GCCGGC G^CCGGC NspI 1724 ACATGC [GA]CATG^[CT] PshBI 1804 ATTAAT AT^TAAT SspI 2762 AATATT AAT^ATT XcmI 560 CCAGAGAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Asp700I 1279 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1629 GAACACCTTC AspHI 2036 GTGCTC G[AT]GC[AT]^C 2287 GAGCAC AspI 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1311 GACAGAGTC BbsI 2833 GAAGAC GAAGAC(2/6) 2940 GTCTTC BbvCI 349 CCTCAGC CCTCAGC(-5/-2) 1525 CCTCAGC BclI 1910 TGATCA T^GATCA 2963 TGATCA BfaI 77 CTAG C^TAG 602 CTAG BpuAI 2833 GAAGAC GAAGAC(2/6) 2940 GTCTTC BsaWI 594 ACCGGA [AT]^CCGG[AT] 702 ACCGGA BsgI 1108 GTGCAG GTGCAG(16/14) 1337 GTGCAG BsiEI 807 CGGCCG CG[GA][CT]^CG 819 CGGCCG BsiHKAI 2036 GTGCTC G[AT]GC[AT]^C 2287 GAGCAC BsrBI 2061 CCGCTC CCGCTC(-3/-3) 2467 GAGCGG BsrDI 167 CATTGC GCAATG(2/0) 2372 CATTGC BsrFI 1730 GCCGGC [GA]^CCGG[CT] 2989 ACCGGC Cfr10I 1730 GCCGGC [GA]^CCGG[CT] 2989 ACCGGC DraIII 1102 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2884 CACTCAGTG EagI 807 CGGCCG C^GGCCG 819 CGGCCG EclXI 807 CGGCCG C^GGCCG 819 CGGCCG Eco52I 807 CGGCCG C^GGCCG 819 CGGCCG FbaI 1910 TGATCA T^GATCA 2963 TGATCA MaeI 77 CTAG C^TAG 602 CTAG MseI 1805 TTAA T^TAA 2462 TTAA PflFI 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1311 GACAGAGTC PpuMI 1832 AGGACCT [GA]G^G[AT]CC[CT] 2110 AGGACCC SmlI 2148 CTCAAG C^T[CT][GA]AG 2407 CTCAAG Tru9I 1805 TTAA T^TAA 2462 TTAA Tth111I 931 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1311 GACAGAGTC XmnI 1279 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1629 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII