(Circular) (Six-base) MAPSORT of: pFN21AE2581.seq Check: 6166 from: 1 to: 7762 pFN21AE2581 7762 bp With 182 enzymes: SgfI * July 22, 2015 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 5572 278 Size: 53 83 186 936 3298 738 2468 Fragments arranged by size: 3298 2468 936 738 186 83 53 AccI GT'mk_AC Cuts at: 1937 4440 5105 1937 Size: 2503 665 4594 Fragments arranged by size: 4594 2503 665 Acc65I G'GTAC_C Cuts at: 5083 5083 Size: 7762 AclI AA'CG_TT Cuts at: 1563 5889 6262 1563 Size: 4326 373 3063 Fragments arranged by size: 4326 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 2577 3001 5825 7209 7477 1632 Size: 550 395 424 2824 1384 268 1917 Fragments arranged by size: 2824 1917 1384 550 424 395 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7565 AflIII A'CryG_T Cuts at: 1203 4435 6661 7638 1203 Size: 3232 2226 977 1327 Fragments arranged by size: 3232 2226 1327 977 AgeI A'CCGG_T Cuts at: 6593 6593 Size: 7762 AhdI GACnn_n'nnGTC Cuts at: 6491 6491 Size: 7762 AleI CACnn'nnGTG Cuts at: 1166 2328 2660 3353 1166 Size: 1162 332 693 5575 Fragments arranged by size: 5575 1162 693 332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7762 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4652 4684 4652 Size: 32 7730 AlwNI CAG_nnn'CTG Cuts at: 2977 3538 3553 3841 5505 7077 2977 Size: 561 15 288 1664 1572 3662 Fragments arranged by size: 3662 1664 1572 561 288 15 ApaLI G'TGCA_C Cuts at: 5819 6975 5819 Size: 1156 6606 ApoI r'AATT_y Cuts at: 1493 3284 4783 4823 5071 5297 5449 7558 1493 Size: 1791 1499 40 248 226 152 2109 1697 Fragments arranged by size: 2109 1791 1697 1499 248 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 3810 6316 160 Size: 872 2778 2506 1606 Fragments arranged by size: 2778 2506 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 AvaI C'yCGr_G Cuts at: 1870 1943 2137 5087 1870 Size: 73 194 2950 4545 Fragments arranged by size: 4545 2950 194 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4633 4666 4633 Size: 33 7729 BamHI G'GATC_C Cuts at: 3961 5092 5459 3961 Size: 1131 367 6264 Fragments arranged by size: 6264 1131 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 3738 4932 5083 6538 Size: 325 217 627 1538 413 1194 151 1455 Cuts at: 6538 618 Size: 1842 Fragments arranged by size: 1842 1538 1455 1194 627 413 325 217 151 BanII G_rGCy'C Cuts at: 1391 2151 3954 4053 5081 1391 Size: 760 1803 99 1028 4072 Fragments arranged by size: 4072 1803 1028 760 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4845 4938 928 Size: 971 2946 93 3752 Fragments arranged by size: 3752 2946 971 93 BbvCI CC'TCA_GC Cuts at: 2355 3531 2355 Size: 1176 6586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5953 5987 1903 Size: 34 50 34 3932 34 3678 Fragments arranged by size: 3932 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5654 6864 5654 Size: 1210 6552 BclI T'GATC_A Cuts at: 1548 3915 4968 1548 Size: 2367 1053 4342 Fragments arranged by size: 4342 2367 1053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 2719 2832 Size: 107 122 71 631 605 821 226 113 Cuts at: 2832 3797 4269 6373 136 Size: 965 472 2104 1525 Fragments arranged by size: 2104 1525 965 821 631 605 472 226 122 113 107 71 BglII A'GATC_T Cuts at: 7757 7757 Size: 7762 BlpI GC'TnA_GC Cuts at: 5179 5179 Size: 7762 Bme1580I G_kGCm'C Cuts at: 1790 2565 2667 4937 5823 6979 1790 Size: 775 102 2270 886 1156 2573 Fragments arranged by size: 2573 2270 1156 886 775 102 BmgBI CAC'GTC Cuts at: 1318 4438 1318 Size: 3120 4642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3347 4172 4936 Size: 416 53 83 399 24 1930 825 764 Cuts at: 4936 5493 6451 442 Size: 557 958 1753 Fragments arranged by size: 1930 1753 958 825 764 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7762 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2331 4306 4613 4763 6422 1239 Size: 966 126 1975 307 150 1659 2579 Fragments arranged by size: 2579 1975 1659 966 307 150 126 Bpu10I CC'TnA_GC Cuts at: 2355 3531 3948 4047 2355 Size: 1176 417 99 6070 Fragments arranged by size: 6070 1176 417 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 4397 5887 6752 7050 4138 Size: 259 1490 865 298 4850 Fragments arranged by size: 4850 1490 865 298 259 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6425 882 Size: 617 4926 2219 Fragments arranged by size: 4926 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2662 5553 493 Size: 711 1458 2891 2702 Fragments arranged by size: 2891 2702 1458 711 BsaBI GATnn'nnATC Cuts at: 7756 7756 Size: 7762 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4340 4599 4831 5569 Size: 53 83 186 936 2807 259 232 738 Cuts at: 5569 5951 6586 275 Size: 382 635 1451 Fragments arranged by size: 2807 1451 936 738 635 382 259 232 186 83 53 BsaWI w'CCGG_w Cuts at: 2599 2707 6195 6593 6867 7014 7425 2599 Size: 108 3488 398 274 147 411 2936 Fragments arranged by size: 3488 2936 411 398 274 147 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2407 2437 3305 3335 3506 Size: 30 165 30 1004 30 868 30 171 Cuts at: 3506 3536 1178 Size: 30 5404 Fragments arranged by size: 5404 1004 868 171 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3327 3386 3512 3620 4586 4883 1186 Size: 2141 59 126 108 966 297 4065 Fragments arranged by size: 4065 2141 966 297 126 108 59 BseYI C'CCAG_C Cuts at: 2683 3264 3475 3546 4272 6965 7461 2683 Size: 581 211 71 726 2693 496 2984 Fragments arranged by size: 2984 2693 726 581 496 211 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3134 3363 1163 Size: 573 1398 229 5562 Fragments arranged by size: 5562 1398 573 229 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 2827 5212 5973 7001 1540 Size: 275 186 814 12 2385 761 1028 2301 Fragments arranged by size: 2385 2301 1028 814 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 4045 4296 5823 5908 6979 4045 Size: 251 1527 85 1071 4828 Fragments arranged by size: 4828 1527 1071 251 85 BsiWI C'GTAC_G Cuts at: 2897 2897 Size: 7762 BsmI GAATG_Cn' Cuts at: 5273 5366 5273 Size: 93 7669 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2565 2667 3954 4045 4053 4296 Size: 399 361 414 102 1287 91 8 243 Cuts at: 4296 4937 5081 5823 5908 6979 1391 Size: 641 144 742 85 1071 2174 Fragments arranged by size: 2174 1287 1071 742 641 414 399 361 243 144 102 91 85 8 BspEI T'CCGG_A Cuts at: 7425 7425 Size: 7762 BspHI T'CATG_A Cuts at: 5651 7381 5651 Size: 1730 6032 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2626 5117 844 Size: 1782 2491 3489 Fragments arranged by size: 3489 2491 1782 BsrBI CCG'CTC Cuts at: 1394 1823 4068 4474 5649 1394 Size: 429 2245 406 1175 3507 Fragments arranged by size: 3507 2245 1175 429 406 BsrDI GCAATG_nn' Cuts at: 65 1248 2171 4376 6257 6431 65 Size: 1183 923 2205 1881 174 1396 Fragments arranged by size: 2205 1881 1396 1183 923 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3735 4994 6406 6593 1700 Size: 110 1925 1259 1412 187 2869 Fragments arranged by size: 2869 1925 1412 1259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7762 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7762 BssSI C'ACGA_G Cuts at: 3339 5822 6834 3339 Size: 2483 1012 4267 Fragments arranged by size: 4267 2483 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7762 BstBI TT'CG_AA Cuts at: 6566 6566 Size: 7762 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7762 BstXI CCAn_nnnn'nTGG Cuts at: 3219 4736 4786 3219 Size: 1517 50 6195 Fragments arranged by size: 6195 1517 50 BstYI r'GATC_y Cuts at: 1972 3699 3961 4852 5092 5459 5844 5861 7313 Size: 1727 262 891 240 367 385 17 1452 Cuts at: 7313 7399 7411 7757 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1727 1452 891 385 367 346 262 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2667 2785 2791 4860 513 Size: 552 1602 118 6 2069 3415 Fragments arranged by size: 3415 2069 1602 552 118 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4045 487 Size: 683 56 2819 4204 Fragments arranged by size: 4204 2819 683 56 BtsI GCAGTG_nn' Cuts at: 3642 5278 6072 6092 3642 Size: 1636 794 20 5312 Fragments arranged by size: 5312 1636 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7762 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7727 DraI TTT'AAA Cuts at: 5066 5066 Size: 7762 DraIII CAC_nnn'GTG Cuts at: 3112 4894 3112 Size: 1782 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3689 6769 809 Size: 507 2373 3080 1802 Fragments arranged by size: 3080 2373 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2788 2812 2824 Size: 54 1425 50 275 18 958 24 12 Cuts at: 2824 3467 3798 4023 4492 4726 4996 5209 6098 Size: 643 331 225 469 234 270 213 889 Cuts at: 6098 7727 8 Size: 1629 43 Fragments arranged by size: 1629 1425 958 889 643 469 331 275 270 234 225 213 54 50 43 24 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2812 2824 5209 1537 Size: 275 1000 12 2385 4090 Fragments arranged by size: 4090 2385 1000 275 12 EarI CTCTTCn'nnn_ Cuts at: 1353 2744 3231 3452 3827 4523 5692 1353 Size: 1391 487 221 375 696 1169 3423 Fragments arranged by size: 3423 1391 1169 696 487 375 221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3643 6349 6723 6869 387 Size: 1064 138 2054 2706 374 146 1280 Fragments arranged by size: 2706 2054 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2205 2331 2577 3001 4306 4613 Size: 393 550 23 126 246 424 1305 307 Cuts at: 4613 4763 5825 6422 7209 7477 1239 Size: 150 1062 597 787 268 1524 Fragments arranged by size: 1524 1305 1062 787 597 550 424 393 307 268 246 150 126 23 EcoO109I rG'GnC_Cy Cuts at: 2891 3118 3403 3838 4116 4299 2891 Size: 227 285 435 278 183 6354 Fragments arranged by size: 6354 435 285 278 227 183 EcoRI G'AATT_C Cuts at: 5071 5449 5071 Size: 378 7384 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3655 3655 Size: 7762 FspI TGC'GCA Cuts at: 6268 6268 Size: 7762 HaeII r_GCGC'y Cuts at: 1678 3001 3792 4462 6909 1678 Size: 1323 791 670 2447 2531 Fragments arranged by size: 2531 2447 1323 791 670 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2277 2309 2723 2755 3305 Size: 32 135 32 705 32 414 32 550 Cuts at: 3305 3337 3647 3679 6403 6435 6477 6509 1373 Size: 32 310 32 2724 32 42 32 2626 Fragments arranged by size: 2724 2626 705 550 414 310 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3694 5106 5352 669 Size: 988 281 1756 1412 246 3079 Fragments arranged by size: 3079 1756 1412 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7762 HpaI GTT'AAC Cuts at: 5352 5352 Size: 7762 KpnI G_GTAC'C Cuts at: 5087 5087 Size: 7762 MfeI C'AATT_G Cuts at: 2296 5361 2296 Size: 3065 4697 MluI A'CGCG_T Cuts at: 7638 7638 Size: 7762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4852 6876 7060 1305 Size: 3547 2024 184 2007 Fragments arranged by size: 3547 2024 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3800 4025 4494 4728 10 Size: 54 1425 343 1968 225 469 234 3044 Fragments arranged by size: 3044 1968 1425 469 343 234 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2660 3353 5720 6079 6238 518 Size: 648 1162 332 693 2367 359 159 2042 Fragments arranged by size: 2367 2042 1162 693 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 2132 2243 3538 3553 4585 5178 5855 7003 Size: 526 111 1295 15 1032 593 677 1148 Cuts at: 7003 7248 1606 Size: 245 2120 Fragments arranged by size: 2120 1295 1148 1032 677 593 526 245 111 15 NaeI GCC'GGC Cuts at: 3737 3737 Size: 7762 NcoI C'CATG_G Cuts at: 513 1065 4860 513 Size: 552 3795 3415 Fragments arranged by size: 3795 3415 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7762 NgoMIV G'CCGG_C Cuts at: 3735 3735 Size: 7762 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7762 NotI GC'GGCC_GC Cuts at: 5209 5209 Size: 7762 NspI r_CATG'y Cuts at: 1132 1229 3733 5120 6665 1132 Size: 97 2504 1387 1545 2229 Fragments arranged by size: 2504 2229 1545 1387 97 PasI CC'CwG_GG Cuts at: 1348 2102 3743 1348 Size: 754 1641 5367 Fragments arranged by size: 5367 1641 754 PciI A'CATG_T Cuts at: 6661 6661 Size: 7762 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6860 PfoI T'CCnGG_A Cuts at: 5007 5007 Size: 7762 PmeI GTTT'AAAC Cuts at: 5066 5066 Size: 7762 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5831 5863 7344 7376 5831 Size: 32 1481 32 6217 Fragments arranged by size: 6217 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3838 4116 3838 Size: 278 7484 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7762 PsiI TTA'TAA Cuts at: 5332 5332 Size: 7762 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4559 4591 4559 Size: 32 7730 PstI C_TGCA'G Cuts at: 830 5114 830 Size: 4284 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7762 PvuII CAG'CTG Cuts at: 2132 2243 3538 3553 4585 2132 Size: 111 1295 15 1032 5309 Fragments arranged by size: 5309 1295 1032 111 15 SalI G'TCGA_C Cuts at: 1936 5104 1936 Size: 3168 4594 SbfI CC_TGCA'GG Cuts at: 5114 5114 Size: 7762 ScaI AGT'ACT Cuts at: 6010 7722 6010 Size: 1712 6050 SfcI C'TryA_G Cuts at: 826 1045 4344 4612 4645 5110 6245 6926 7117 Size: 219 3299 268 33 465 1135 681 191 Cuts at: 7117 826 Size: 1471 Fragments arranged by size: 3299 1471 1135 681 465 268 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7762 SmaI CCC'GGG Cuts at: 1872 5089 1872 Size: 3217 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4153 4412 5866 6767 7029 820 Size: 197 926 2210 259 1454 901 262 1553 Fragments arranged by size: 2210 1553 1454 926 901 262 259 197 SnaBI TAC'GTA Cuts at: 493 5553 493 Size: 5060 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7762 SphI G_CATG'C Cuts at: 1132 5120 1132 Size: 3988 3774 SspI AAT'ATT Cuts at: 5 52 4769 5686 5 Size: 47 4717 917 2081 Fragments arranged by size: 4717 2081 917 47 StuI AGG'CCT Cuts at: 1517 2035 3463 4957 1517 Size: 518 1428 1494 4322 Fragments arranged by size: 4322 1494 1428 518 StyI C'CwwG_G Cuts at: 513 1065 2124 2226 3399 3561 3801 4254 4860 Size: 552 1059 102 1173 162 240 453 606 Cuts at: 4860 5201 513 Size: 341 3074 Fragments arranged by size: 3074 1173 1059 606 552 453 341 240 162 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 2392 3728 3756 4264 4853 5005 Size: 1255 484 328 1336 28 508 589 152 Cuts at: 5005 5789 5806 5959 6144 6589 325 Size: 784 17 153 185 445 1498 Fragments arranged by size: 1498 1336 1255 784 589 508 484 445 328 185 153 152 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 6008 7720 Size: 274 80 33 51 1443 930 3101 1712 Cuts at: 7720 96 Size: 138 Fragments arranged by size: 3101 1712 1443 930 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3506 3538 3981 4013 7722 7754 3506 Size: 32 443 32 3709 32 3514 Fragments arranged by size: 3709 3514 443 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2939 3319 1316 Size: 1623 380 5759 Fragments arranged by size: 5759 1623 380 XbaI T'CTAG_A Cuts at: 5098 5098 Size: 7762 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2572 Size: 7762 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7762 XmaI C'CCGG_G Cuts at: 1870 5087 1870 Size: 3217 4545 XmnI GAAnn'nnTTC Cuts at: 3288 3638 5891 3288 Size: 350 2253 5159 Fragments arranged by size: 5159 2253 350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 5570 276 Size: 53 83 186 936 3298 738 2468 Fragments arranged by size: 3298 2468 936 738 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI