(Linear) (Six-base) MAPSORT of: KIEE2581.seq Check: 4114 from: 1 to: 3057 KIEE2581.orf With 182 enzymes: SgfI * July 22, 2015 09:50 .. AatII G_ACGT'C Cuts at: 0 2829 3057 Size: 2829 228 AccI GT'mk_AC Cuts at: 0 2435 3057 Size: 2435 622 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 572 996 3057 Size: 177 395 424 2061 Fragments arranged by size: 2061 424 395 177 AflIII A'CryG_T Cuts at: 0 2430 3057 Size: 2430 627 AleI CACnn'nnGTG Cuts at: 0 323 655 1348 3057 Size: 323 332 693 1709 Fragments arranged by size: 1709 693 332 323 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2647 2679 3057 Size: 2647 32 378 Fragments arranged by size: 2647 378 32 AlwNI CAG_nnn'CTG Cuts at: 0 972 1533 1548 1836 3057 Size: 972 561 15 288 1221 Fragments arranged by size: 1221 972 561 288 15 ApoI r'AATT_y Cuts at: 0 1279 2778 2818 3057 Size: 1279 1499 40 239 Fragments arranged by size: 1499 1279 239 40 AseI AT'TA_AT Cuts at: 0 1805 3057 Size: 1805 1252 AvaI C'yCGr_G Cuts at: 0 132 3057 Size: 132 2925 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2628 2661 3057 Size: 2628 33 396 Fragments arranged by size: 2628 396 33 BamHI G'GATC_C Cuts at: 0 1956 3057 Size: 1956 1101 BanI G'GyrC_C Cuts at: 0 1320 1733 2927 3057 Size: 1320 413 1194 130 Fragments arranged by size: 1320 1194 413 130 BanII G_rGCy'C Cuts at: 0 146 1949 2048 3057 Size: 146 1803 99 1009 Fragments arranged by size: 1803 1009 146 99 BbsI GAAGACnn'nnnn_ Cuts at: 0 2840 2933 3057 Size: 2840 93 124 Fragments arranged by size: 2840 124 93 BbvCI CC'TCA_GC Cuts at: 0 350 1526 3057 Size: 350 1176 1531 Fragments arranged by size: 1531 1176 350 BclI T'GATC_A Cuts at: 0 1910 2963 3057 Size: 1910 1053 94 Fragments arranged by size: 1910 1053 94 BglI GCCn_nnn'nGGC Cuts at: 0 488 714 827 1792 2264 3057 Size: 488 226 113 965 472 793 Fragments arranged by size: 965 793 488 472 226 113 Bme1580I G_kGCm'C Cuts at: 0 560 662 2932 3057 Size: 560 102 2270 125 Fragments arranged by size: 2270 560 125 102 BmgBI CAC'GTC Cuts at: 0 2433 3057 Size: 2433 624 BmrI ACTGGGnnnn_n' Cuts at: 0 1342 2167 2931 3057 Size: 1342 825 764 126 Fragments arranged by size: 1342 825 764 126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 326 2301 2608 2758 3057 Size: 200 126 1975 307 150 299 Fragments arranged by size: 1975 307 299 200 150 126 Bpu10I CC'TnA_GC Cuts at: 0 350 1526 1943 2042 3057 Size: 350 1176 417 99 1015 Fragments arranged by size: 1176 1015 417 350 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2133 2392 3057 Size: 2133 259 665 Fragments arranged by size: 2133 665 259 BsaAI yAC'GTr Cuts at: 0 657 3057 Size: 657 2400 BsaHI Gr'CG_yC Cuts at: 0 2335 2594 2826 3057 Size: 2335 259 232 231 Fragments arranged by size: 2335 259 232 231 BsaWI w'CCGG_w Cuts at: 0 594 702 3057 Size: 594 108 2355 Fragments arranged by size: 2355 594 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 402 432 1300 1330 1501 1531 3057 Size: 402 30 868 30 171 30 1526 Fragments arranged by size: 1526 868 402 171 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1322 1381 1507 1615 2581 2878 3057 Size: 1322 59 126 108 966 297 179 Fragments arranged by size: 1322 966 297 179 126 108 59 BseYI C'CCAG_C Cuts at: 0 678 1259 1470 1541 2267 3057 Size: 678 581 211 71 726 790 Fragments arranged by size: 790 726 678 581 211 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1358 3057 Size: 1129 229 1699 Fragments arranged by size: 1699 1129 229 BsiEI CG_ry'CG Cuts at: 0 810 822 3057 Size: 810 12 2235 Fragments arranged by size: 2235 810 12 BsiHKAI G_wGCw'C Cuts at: 0 2040 2291 3057 Size: 2040 251 766 Fragments arranged by size: 2040 766 251 BsiWI C'GTAC_G Cuts at: 0 892 3057 Size: 892 2165 Bsp1286I G_dGCh'C Cuts at: 0 146 560 662 1949 2040 2048 2291 2932 Size: 146 414 102 1287 91 8 243 641 Cuts at: 2932 3057 Size: 125 Fragments arranged by size: 1287 641 414 243 146 125 102 91 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 621 3057 Size: 621 2436 BsrBI CCG'CTC Cuts at: 0 2063 2469 3057 Size: 2063 406 588 Fragments arranged by size: 2063 588 406 BsrDI GCAATG_nn' Cuts at: 0 166 2371 3057 Size: 166 2205 686 Fragments arranged by size: 2205 686 166 BsrFI r'CCGG_y Cuts at: 0 1730 2989 3057 Size: 1730 1259 68 Fragments arranged by size: 1730 1259 68 BssSI C'ACGA_G Cuts at: 0 1334 3057 Size: 1334 1723 BstXI CCAn_nnnn'nTGG Cuts at: 0 1214 2731 2781 3057 Size: 1214 1517 50 276 Fragments arranged by size: 1517 1214 276 50 BstYI r'GATC_y Cuts at: 0 1694 1956 2847 3057 Size: 1694 262 891 210 Fragments arranged by size: 1694 891 262 210 BtgI C'CryG_G Cuts at: 0 662 780 786 2855 3057 Size: 662 118 6 2069 202 Fragments arranged by size: 2069 662 202 118 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2040 3057 Size: 2040 1017 BtsI GCAGTG_nn' Cuts at: 0 1637 3057 Size: 1637 1420 DraIII CAC_nnn'GTG Cuts at: 0 1107 2889 3057 Size: 1107 1782 168 Fragments arranged by size: 1782 1107 168 DrdI GACnn_nn'nnGTC Cuts at: 0 1684 3057 Size: 1684 1373 EaeI y'GGCC_r Cuts at: 0 783 807 819 1462 1793 2018 2487 2721 Size: 783 24 12 643 331 225 469 234 Cuts at: 2721 2991 3057 Size: 270 66 Fragments arranged by size: 783 643 469 331 270 234 225 66 24 12 EagI C'GGCC_G Cuts at: 0 807 819 3057 Size: 807 12 2238 Fragments arranged by size: 2238 807 12 EarI CTCTTCn'nnn_ Cuts at: 0 739 1226 1447 1822 2518 3057 Size: 739 487 221 375 696 539 Fragments arranged by size: 739 696 539 487 375 221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1638 3057 Size: 1638 1419 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 200 326 572 996 2301 2608 2758 Size: 177 23 126 246 424 1305 307 150 Cuts at: 2758 3057 Size: 299 Fragments arranged by size: 1305 424 307 299 246 177 150 126 23 EcoO109I rG'GnC_Cy Cuts at: 0 886 1113 1398 1833 2111 2294 3057 Size: 886 227 285 435 278 183 763 Fragments arranged by size: 886 763 435 285 278 227 183 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1650 3057 Size: 1650 1407 HaeII r_GCGC'y Cuts at: 0 996 1787 2457 3057 Size: 996 791 670 600 Fragments arranged by size: 996 791 670 600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 272 304 718 750 1300 1332 1642 1674 Size: 272 32 414 32 550 32 310 32 Cuts at: 1674 3057 Size: 1383 Fragments arranged by size: 1383 550 414 310 272 32 32 32 32 HincII GTy'rAC Cuts at: 0 1689 3057 Size: 1689 1368 MfeI C'AATT_G Cuts at: 0 291 3057 Size: 291 2766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2847 3057 Size: 2847 210 MscI TGG'CCA Cuts at: 0 1795 2020 2489 2723 3057 Size: 1795 225 469 234 334 Fragments arranged by size: 1795 469 334 234 225 MslI CAynn'nnrTG Cuts at: 0 323 655 1348 3057 Size: 323 332 693 1709 Fragments arranged by size: 1709 693 332 323 MspA1I CmG'CkG Cuts at: 0 127 238 1533 1548 2580 3057 Size: 127 111 1295 15 1032 477 Fragments arranged by size: 1295 1032 477 127 111 15 NaeI GCC'GGC Cuts at: 0 1732 3057 Size: 1732 1325 NcoI C'CATG_G Cuts at: 0 2855 3057 Size: 2855 202 NgoMIV G'CCGG_C Cuts at: 0 1730 3057 Size: 1730 1327 NspI r_CATG'y Cuts at: 0 1728 3057 Size: 1728 1329 PasI CC'CwG_GG Cuts at: 0 97 1738 3057 Size: 97 1641 1319 Fragments arranged by size: 1641 1319 97 PfoI T'CCnGG_A Cuts at: 0 3002 3057 Size: 3002 55 PpuMI rG'GwC_Cy Cuts at: 0 1833 2111 3057 Size: 1833 278 946 Fragments arranged by size: 1833 946 278 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2554 2586 3057 Size: 2554 32 471 Fragments arranged by size: 2554 471 32 PvuII CAG'CTG Cuts at: 0 127 238 1533 1548 2580 3057 Size: 127 111 1295 15 1032 477 Fragments arranged by size: 1295 1032 477 127 111 15 SfcI C'TryA_G Cuts at: 0 2339 2607 2640 3057 Size: 2339 268 33 417 Fragments arranged by size: 2339 417 268 33 SmlI C'TyrA_G Cuts at: 0 2148 2407 3057 Size: 2148 259 650 Fragments arranged by size: 2148 650 259 SspI AAT'ATT Cuts at: 0 2764 3057 Size: 2764 293 StuI AGG'CCT Cuts at: 0 30 1458 2952 3057 Size: 30 1428 1494 105 Fragments arranged by size: 1494 1428 105 30 StyI C'CwwG_G Cuts at: 0 119 221 1394 1556 1796 2249 2855 3057 Size: 119 102 1173 162 240 453 606 202 Fragments arranged by size: 1173 606 453 240 202 162 119 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 59 387 1723 1751 2259 2848 3000 3057 Size: 59 328 1336 28 508 589 152 57 Fragments arranged by size: 1336 589 508 328 152 59 57 28 TatI w'GTAC_w Cuts at: 0 902 3057 Size: 902 2155 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1501 1533 1976 2008 3057 Size: 1501 32 443 32 1049 Fragments arranged by size: 1501 1049 443 32 32 Tth111I GACn'n_nGTC Cuts at: 0 934 1314 3057 Size: 934 380 1743 Fragments arranged by size: 1743 934 380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 567 3057 Size: 567 2490 XmnI GAAnn'nnTTC Cuts at: 0 1283 1633 3057 Size: 1283 350 1424 Fragments arranged by size: 1424 1283 350 ZraI GAC'GTC Cuts at: 0 2827 3057 Size: 2827 230 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpuMI PsrI PvuII SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvrII BbeI BcgI BciVI BfrBI BglII BlpI BmtI BplI BsaI BsaBI BsmI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmaI