################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccI AccIII Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BclI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HindIII HpaI MamI MfeI MflI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshBI PvuI RsrII SalI SapI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1794 GGTACC G^GTACC AclI 2446 AACGTT AA^CGTT AcsI 1219 GAATTT [GA]^AATT[CT] AflII 2584 CTTAAG C^TTAAG AflIII 1788 ACACGT A^C[GA][CT]GT AgeI 661 ACCGGT A^CCGGT AhdI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 177 GGGCCC GGGCC^C ApoI 1219 GAATTT [GA]^AATT[CT] Asp718I 1794 GGTACC G^GTACC AspEI 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2077 GGCGCC GGCGC^C BbrPI 1789 CACGTG CAC^GTG BbvCI 797 CCTCAGC CCTCAGC(-5/-2) BciVI 2000 GGATAC GTATCC(6/5) BfrI 2584 CTTAAG C^TTAAG BsaAI 1789 CACGTG [CT]AC^GT[GA] BsmBI 958 GAGACG CGTCTC(1/5) BsrBI 2159 CCGCTC CCGCTC(-3/-3) DpnII 2919 GATC ^GATC Eam1105I 2535 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1426 GTCAAC GT[CT]^[GA]AC HindII 1426 GTCAAC GT[CT]^[GA]AC KasI 2077 GGCGCC G^GCGCC KpnI 1794 GGTACC GGTAC^C MboI 2919 GATC ^GATC NarI 2077 GGCGCC GG^CGCC NcoI 376 CCATGG C^CATGG NdeII 2919 GATC ^GATC PflMI 528 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 661 ACCGGT A^CCGGT PmaCI 1789 CACGTG CAC^GTG PmlI 1789 CACGTG CAC^GTG PshAI 1273 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2446 AACGTT AA^CGTT Sau3AI 2919 GATC ^GATC SbfI 519 CCTGCAGG CCTGCA^GG ScaI 1481 AGTACT AGT^ACT SfiI 424 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2077 GGCGCC GGC^GCC Sse8387I 519 CCTGCAGG CCTGCA^GG Van91I 528 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 461 CCACCACGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 123 GGCGTC G[GA]^CG[CT]C 2077 GGCGCC AocI 382 CCTCAGG CC^T[ACGT]AGG 2143 CCTGAGG Asp700I 1647 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2684 GAAATAATTC BcgI 957 CGAGACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2337 GCACCTGGGTCG BglI 425 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 802 GCCTCTACGGC BsaHI 123 GGCGTC G[GA]^CG[CT]C 2077 GGCGCC BsaI 940 GAGACC GGTCTC(1/5) 3112 GGTCTC BsaWI 661 ACCGGT [AT]^CCGG[AT] 2273 TCCGGT BspHI 1281 TCATGA T^CATGA 1529 TCATGA BsrDI 1799 CATTGC GCAATG(2/0) 3173 GCAATG BsrFI 661 ACCGGT [GA]^CCGG[CT] 2390 ACCGGC Bsu36I 382 CCTCAGG CC^T[ACGT]AGG 2143 CCTGAGG Cfr10I 661 ACCGGT [GA]^CCGG[CT] 2390 ACCGGC Eco81I 382 CCTCAGG CC^T[ACGT]AGG 2143 CCTGAGG EcoT22I 2046 ATGCAT ATGCA^T 2991 ATGCAT HaeII 2077 GGCGCC [GA]GCGC^[CT] 3178 GGCGCT HgaI 616 GACGC GACGC(5/10) 124 GCGTC Hin1I 123 GGCGTC G[GA]^CG[CT]C 2077 GGCGCC KspI 2116 CCGCGG CCGC^GG 2843 CCGCGG MseI 845 TTAA T^TAA 2585 TTAA NsiI 2046 ATGCAT ATGCA^T 2991 ATGCAT NspI 21 GCATGT [GA]CATG^[CT] 2762 ACATGC PpuMI 1559 AGGACCT [GA]G^G[AT]CC[CT] 2713 GGGACCC RcaI 1281 TCATGA T^CATGA 1529 TCATGA SacI 718 GAGCTC GAGCT^C 3009 GAGCTC SacII 2116 CCGCGG CCGC^GG 2843 CCGCGG TaqI 1667 TCGA T^CGA 2984 TCGA Tru9I 845 TTAA T^TAA 2585 TTAA TthHB8I 1667 TCGA T^CGA 2984 TCGA XmnI 1647 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2684 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII RsaI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI XmaCI XmaI