(Circular) (Six-base) MAPSORT of: pFN21AE5819.seq Check: 4362 from: 1 to: 7894 pFN21AE5819 7894 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5704 278 Size: 53 83 186 936 4168 2468 Fragments arranged by size: 4168 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5237 1937 Size: 3300 4594 Acc65I G'GTAC_C Cuts at: 3799 5215 3799 Size: 1416 6478 AclI AA'CG_TT Cuts at: 1563 4452 6021 6394 1563 Size: 2889 1569 373 3063 Fragments arranged by size: 3063 2889 1569 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2727 2997 3033 3601 4211 4764 5957 Size: 557 538 270 36 568 610 553 1193 Cuts at: 5957 7341 7609 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1193 610 568 557 553 538 270 268 36 AflII C'TTAA_G Cuts at: 820 1017 4589 820 Size: 197 3572 4125 Fragments arranged by size: 4125 3572 197 AflIII A'CryG_T Cuts at: 1203 3793 6793 7770 1203 Size: 2590 3000 977 1327 Fragments arranged by size: 3000 2590 1327 977 AgeI A'CCGG_T Cuts at: 2666 6725 2666 Size: 4059 3835 AhdI GACnn_n'nnGTC Cuts at: 4545 6623 4545 Size: 2078 5816 AleI CACnn'nnGTG Cuts at: 1166 3727 5071 1166 Size: 2561 1344 3989 Fragments arranged by size: 3989 2561 1344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4149 1184 Size: 2965 4929 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 5024 5056 2102 Size: 32 2890 32 4940 Fragments arranged by size: 4940 2890 32 32 AlwNI CAG_nnn'CTG Cuts at: 2105 2167 2355 3056 5637 7209 2105 Size: 62 188 701 2581 1572 2790 Fragments arranged by size: 2790 2581 1572 701 188 62 ApaI G_GGCC'C Cuts at: 2186 2186 Size: 7894 ApaLI G'TGCA_C Cuts at: 5951 7107 5951 Size: 1156 6738 ApoI r'AATT_y Cuts at: 1493 3224 5203 5429 5581 7690 1493 Size: 1731 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1731 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6448 160 Size: 872 5416 1606 Fragments arranged by size: 5416 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 AvaI C'yCGr_G Cuts at: 1870 1943 2197 2225 2455 2762 3127 4511 5219 Size: 73 254 28 230 307 365 1384 708 Cuts at: 5219 1870 Size: 4545 Fragments arranged by size: 4545 1384 708 365 307 254 230 73 28 BamHI G'GATC_C Cuts at: 5224 5591 5224 Size: 367 7527 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3799 4082 4784 5155 5215 Size: 325 217 627 2012 283 702 371 60 Cuts at: 5215 6670 618 Size: 1455 1842 Fragments arranged by size: 2012 1842 1455 702 627 371 325 283 217 60 BanII G_rGCy'C Cuts at: 1391 2186 2204 2235 2727 2763 3128 3470 5018 Size: 795 18 31 492 36 365 342 1548 Cuts at: 5018 5066 5213 1391 Size: 48 147 4072 Fragments arranged by size: 4072 1548 795 492 365 342 147 48 36 31 18 BbeI G_GCGC'C Cuts at: 4086 4086 Size: 7894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2885 3021 3196 4702 928 Size: 971 986 136 175 1506 4120 Fragments arranged by size: 4120 1506 986 971 175 136 BbvCI CC'TCA_GC Cuts at: 2803 2803 Size: 7894 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2951 2985 4331 4365 6085 6119 1903 Size: 34 1014 34 1346 34 1720 34 3678 Fragments arranged by size: 3678 1720 1346 1014 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3999 5786 6996 3999 Size: 1787 1210 4897 Fragments arranged by size: 4897 1787 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7894 BfrBI ATG'CAT Cuts at: 4053 4998 4053 Size: 945 6949 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2436 2813 6505 Size: 107 122 71 631 605 764 377 3692 Cuts at: 6505 136 Size: 1525 Fragments arranged by size: 3692 1525 764 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 7889 7889 Size: 7894 BlpI GC'TnA_GC Cuts at: 2832 3122 4256 5017 5311 2832 Size: 290 1134 761 294 5415 Fragments arranged by size: 5415 1134 761 294 290 Bme1580I G_kGCm'C Cuts at: 1790 2186 2462 5158 5955 7111 1790 Size: 396 276 2696 797 1156 2573 Fragments arranged by size: 2696 2573 1156 797 396 276 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7894 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3661 3842 5625 Size: 416 53 83 399 24 2244 181 1783 Cuts at: 5625 6583 442 Size: 958 1753 Fragments arranged by size: 2244 1783 1753 958 416 399 181 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7894 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2097 4195 2097 Size: 2098 5796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 3509 4159 4190 4840 6554 1239 Size: 1259 1011 650 31 650 1714 2579 Fragments arranged by size: 2579 1714 1259 1011 650 650 31 Bpu10I CC'TnA_GC Cuts at: 2803 3469 3989 5093 2803 Size: 666 520 1104 5604 Fragments arranged by size: 5604 1104 666 520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 4430 6019 6884 7182 3005 Size: 1425 1589 865 298 3717 Fragments arranged by size: 3717 1589 1425 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2939 5123 6557 882 Size: 617 1440 2184 1434 2219 Fragments arranged by size: 2219 2184 1440 1434 617 BsaAI yAC'GTr Cuts at: 493 1204 3796 5685 493 Size: 711 2592 1889 2702 Fragments arranged by size: 2702 2592 1889 711 BsaBI GATnn'nnATC Cuts at: 7888 7888 Size: 7894 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2129 4083 5701 6083 Size: 53 83 186 936 596 1954 1618 382 Cuts at: 6083 6718 275 Size: 635 1451 Fragments arranged by size: 1954 1618 1451 936 635 596 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2666 4278 6327 6725 6999 7146 7557 2666 Size: 1612 2049 398 274 147 411 3003 Fragments arranged by size: 3003 2049 1612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 2477 2507 2714 Size: 30 165 30 699 30 345 30 207 Cuts at: 2714 2744 2899 2929 3129 3159 4508 4538 5024 Size: 30 155 30 200 30 1349 30 486 Cuts at: 5024 5054 1178 Size: 30 4018 Fragments arranged by size: 4018 1349 699 486 345 207 200 165 155 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2904 3150 3228 3231 3868 4529 5032 Size: 1057 661 246 78 3 637 661 503 Cuts at: 5032 5116 5152 1186 Size: 84 36 3928 Fragments arranged by size: 3928 1057 661 661 637 503 246 84 78 36 3 BseYI C'CCAG_C Cuts at: 2418 4078 4876 4947 5088 5113 7097 7593 2418 Size: 1660 798 71 141 25 1984 496 2719 Fragments arranged by size: 2719 1984 1660 798 496 141 71 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3069 3248 4082 1163 Size: 573 1333 179 834 4975 Fragments arranged by size: 4975 1333 834 573 179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5344 6105 7133 1540 Size: 275 186 3343 761 1028 2301 Fragments arranged by size: 3343 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 4344 5018 5955 6040 7111 2727 Size: 1617 674 937 85 1071 3510 Fragments arranged by size: 3510 1617 1071 937 674 85 BsmI GAATG_Cn' Cuts at: 5405 5498 5405 Size: 93 7801 BsmBI CGTCTCn'nnnn_ Cuts at: 2957 2957 Size: 7894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2204 2235 2462 2727 2763 3128 Size: 399 396 18 31 227 265 36 365 Cuts at: 3128 3470 4344 5018 5066 5158 5213 5955 6040 Size: 342 874 674 48 92 55 742 85 Cuts at: 6040 7111 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 874 742 674 399 396 365 342 265 227 92 85 55 48 36 31 18 BspEI T'CCGG_A Cuts at: 7557 7557 Size: 7894 BspHI T'CATG_A Cuts at: 3286 3534 5783 7513 3286 Size: 248 2249 1730 3667 Fragments arranged by size: 3667 2249 1730 248 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5249 844 Size: 4405 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4166 5781 1394 Size: 429 2343 1615 3507 Fragments arranged by size: 3507 2343 1615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 5185 6389 6563 65 Size: 1183 2555 1382 1204 174 1396 Fragments arranged by size: 2555 1396 1382 1204 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2666 4395 6538 6725 1700 Size: 110 856 1729 2143 187 2869 Fragments arranged by size: 2869 2143 1729 856 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7894 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7894 BssSI C'ACGA_G Cuts at: 5954 6966 5954 Size: 1012 6882 BstAPI GCAn_nnn'nTGC Cuts at: 794 2064 2822 3315 4252 4944 794 Size: 1270 758 493 937 692 3744 Fragments arranged by size: 3744 1270 937 758 692 493 BstBI TT'CG_AA Cuts at: 6698 6698 Size: 7894 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7894 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2476 3280 4001 2426 Size: 50 804 721 6319 Fragments arranged by size: 6319 804 721 50 BstYI r'GATC_y Cuts at: 1972 5224 5591 5976 5993 7445 7531 7543 7889 Size: 3252 367 385 17 1452 86 12 346 Cuts at: 7889 1972 Size: 1977 Fragments arranged by size: 3252 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2388 4149 2388 Size: 1761 6133 BtgI C'CryG_G Cuts at: 513 1065 2004 2381 4121 4848 513 Size: 552 939 377 1740 727 3559 Fragments arranged by size: 3559 1740 939 727 552 377 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7155 Fragments arranged by size: 7155 683 56 BtsI GCAGTG_nn' Cuts at: 2929 4099 4926 5410 6204 6224 2929 Size: 1170 827 484 794 20 4599 Fragments arranged by size: 4599 1170 827 794 484 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7894 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2491 2526 529 Size: 35 1927 35 5897 Fragments arranged by size: 5897 1927 35 35 DraI TTT'AAA Cuts at: 5198 5198 Size: 7894 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6901 809 Size: 507 5585 1802 Fragments arranged by size: 5585 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 2556 4977 Size: 54 1425 50 275 18 607 119 2421 Cuts at: 4977 5341 6230 7859 8 Size: 364 889 1629 43 Fragments arranged by size: 2421 1629 1425 889 607 364 275 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5341 1537 Size: 275 3529 4090 Fragments arranged by size: 4090 3529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3174 3758 4280 5824 1353 Size: 1821 584 522 1544 3423 Fragments arranged by size: 3423 1821 1544 584 522 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6481 6855 7001 387 Size: 1064 138 4892 374 146 1280 Fragments arranged by size: 4892 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2725 5016 2725 Size: 2291 5603 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2498 2727 2997 3033 3509 3601 Size: 393 557 309 229 270 36 476 92 Cuts at: 3601 4159 4190 4211 4764 4840 5957 6554 7341 Size: 558 31 21 553 76 1117 597 787 Cuts at: 7341 7609 1239 Size: 268 1524 Fragments arranged by size: 1524 1117 787 597 558 557 553 476 393 309 270 268 229 92 76 36 31 21 EcoNI CCTnn'n_nnAGG Cuts at: 2654 3572 5171 2654 Size: 918 1599 5377 Fragments arranged by size: 5377 1599 918 EcoO109I rG'GnC_Cy Cuts at: 2182 2799 3565 4719 2182 Size: 617 766 1154 5357 Fragments arranged by size: 5357 1154 766 617 EcoRI G'AATT_C Cuts at: 5203 5581 5203 Size: 378 7516 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4739 4739 Size: 7894 FspI TGC'GCA Cuts at: 6400 6400 Size: 7894 HaeII r_GCGC'y Cuts at: 1678 4086 5187 7041 1678 Size: 2408 1101 1854 2531 Fragments arranged by size: 2531 2408 1854 1101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 3947 3979 4508 Size: 32 135 32 905 32 1438 32 529 Cuts at: 4508 4531 4540 4563 5048 5080 6535 6567 6609 Size: 23 9 23 485 32 1455 32 42 Cuts at: 6609 6641 1373 Size: 32 2626 Fragments arranged by size: 2626 1455 1438 905 529 485 135 42 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3433 5238 5484 669 Size: 988 281 1495 1805 246 3079 Fragments arranged by size: 3079 1805 1495 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7894 HpaI GTT'AAC Cuts at: 5484 5484 Size: 7894 KasI G'GCGC_C Cuts at: 4082 4082 Size: 7894 KpnI G_GTAC'C Cuts at: 3803 5219 3803 Size: 1416 6478 MfeI C'AATT_G Cuts at: 5493 5493 Size: 7894 MluI A'CGCG_T Cuts at: 7770 7770 Size: 7894 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7008 7192 1305 Size: 5703 184 2007 Fragments arranged by size: 5703 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2439 2558 4979 10 Size: 54 1425 343 607 119 2421 2925 Fragments arranged by size: 2925 2421 1425 607 343 119 54 MslI CAynn'nnrTG Cuts at: 518 1166 3727 4131 5071 5852 6211 6370 518 Size: 648 2561 404 940 781 359 159 2042 Fragments arranged by size: 2561 2042 940 781 648 404 359 159 MspA1I CmG'CkG Cuts at: 1606 3053 3438 3593 3727 4123 4850 5071 5113 Size: 1447 385 155 134 396 727 221 42 Cuts at: 5113 5310 5987 7135 7380 1606 Size: 197 677 1148 245 2120 Fragments arranged by size: 2120 1447 1148 727 677 396 385 245 221 197 155 134 42 NarI GG'CG_CC Cuts at: 4083 4083 Size: 7894 NcoI C'CATG_G Cuts at: 513 1065 2004 2381 513 Size: 552 939 377 6026 Fragments arranged by size: 6026 939 552 377 NdeI CA'TA_TG Cuts at: 387 387 Size: 7894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7894 NotI GC'GGCC_GC Cuts at: 5341 5341 Size: 7894 NsiI A_TGCA'T Cuts at: 4055 5000 4055 Size: 945 6949 NspI r_CATG'y Cuts at: 1132 1229 2030 4771 5252 6797 1132 Size: 97 801 2741 481 1545 2229 Fragments arranged by size: 2741 2229 1545 801 481 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7894 PciI A'CATG_T Cuts at: 6793 6793 Size: 7894 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 1108 Size: 1431 6463 PfoI T'CCnGG_A Cuts at: 2249 2592 3099 4194 4368 4402 4485 2249 Size: 343 507 1095 174 34 83 5658 Fragments arranged by size: 5658 1095 507 343 174 83 34 PmeI GTTT'AAAC Cuts at: 5198 5198 Size: 7894 PmlI CAC'GTG Cuts at: 3796 3796 Size: 7894 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2102 2134 3377 3409 5024 5056 5963 5995 7476 Size: 32 1243 32 1615 32 907 32 1481 Cuts at: 7476 7508 2102 Size: 32 2488 Fragments arranged by size: 2488 1615 1481 1243 907 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3565 4719 3565 Size: 1154 6740 PshAI GACnn'nnGTC Cuts at: 1613 3282 1613 Size: 1669 6225 PsiI TTA'TAA Cuts at: 3369 5464 3369 Size: 2095 5799 PspOMI G'GGCC_C Cuts at: 2182 2182 Size: 7894 PstI C_TGCA'G Cuts at: 830 2060 2089 2529 2778 3630 4574 4634 4669 Size: 1230 29 440 249 852 944 60 35 Cuts at: 4669 5246 830 Size: 577 3478 Fragments arranged by size: 3478 1230 944 852 577 440 249 60 35 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7894 PvuII CAG'CTG Cuts at: 3053 3727 5071 3053 Size: 674 1344 5876 Fragments arranged by size: 5876 1344 674 SacI G_AGCT'C Cuts at: 2727 5018 2727 Size: 2291 5603 SacII CC_GC'GG Cuts at: 4124 4851 4124 Size: 727 7167 SalI G'TCGA_C Cuts at: 1936 5236 1936 Size: 3300 4594 SanDI GG'GwC_CC Cuts at: 4719 4719 Size: 7894 SbfI CC_TGCA'GG Cuts at: 2529 5246 2529 Size: 2717 5177 ScaI AGT'ACT Cuts at: 3488 6142 7854 3488 Size: 2654 1712 3528 Fragments arranged by size: 3528 2654 1712 SexAI A'CCwGG_T Cuts at: 3567 4029 4311 3567 Size: 462 282 7150 Fragments arranged by size: 7150 462 282 SfcI C'TryA_G Cuts at: 826 1045 2056 2085 2525 2774 2826 3626 4570 Size: 219 1011 29 440 249 52 800 944 Cuts at: 4570 4630 4665 5242 6377 7058 7249 826 Size: 60 35 577 1135 681 191 1471 Fragments arranged by size: 1471 1135 1011 944 800 681 577 440 249 219 191 60 52 35 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2436 2436 Size: 7894 SfoI GGC'GCC Cuts at: 4084 4084 Size: 7894 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7894 SmaI CCC'GGG Cuts at: 1872 2199 2457 2764 3129 5221 1872 Size: 327 258 307 365 2092 4545 Fragments arranged by size: 4545 2092 365 327 307 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 3020 4409 4589 5998 6899 7161 Size: 197 926 1077 1389 180 1409 901 262 Cuts at: 7161 820 Size: 1553 Fragments arranged by size: 1553 1409 1389 1077 926 901 262 197 180 SnaBI TAC'GTA Cuts at: 493 5685 493 Size: 5192 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7894 SphI G_CATG'C Cuts at: 1132 5252 1132 Size: 4120 3774 SspI AAT'ATT Cuts at: 5 52 5818 5 Size: 47 5766 2081 Fragments arranged by size: 5766 2081 47 StuI AGG'CCT Cuts at: 1517 2649 3873 4520 1517 Size: 1132 1224 647 4891 Fragments arranged by size: 4891 1224 1132 647 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2381 2955 4000 4714 5172 Size: 552 939 30 347 574 1045 714 458 Cuts at: 5172 5333 513 Size: 161 3074 Fragments arranged by size: 3074 1045 939 714 574 552 458 347 161 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3749 5921 5938 6091 6276 6721 Size: 1255 2100 69 2172 17 153 185 445 Cuts at: 6721 325 Size: 1498 Fragments arranged by size: 2172 2100 1498 1255 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3486 3744 6140 Size: 274 80 33 51 1443 1509 258 2396 Cuts at: 6140 7852 96 Size: 1712 138 Fragments arranged by size: 2396 1712 1509 1443 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3129 3161 3920 3952 7854 7886 3129 Size: 32 759 32 3902 32 3137 Fragments arranged by size: 3902 3137 759 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7894 XbaI T'CTAG_A Cuts at: 5230 5230 Size: 7894 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 7894 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7894 XmaI C'CCGG_G Cuts at: 1870 2197 2455 2762 3127 5219 1870 Size: 327 258 307 365 2092 4545 Fragments arranged by size: 4545 2092 365 327 307 258 XmnI GAAnn'nnTTC Cuts at: 3656 4693 6023 3656 Size: 1037 1330 5527 Fragments arranged by size: 5527 1330 1037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5702 276 Size: 53 83 186 936 4168 2468 Fragments arranged by size: 4168 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SapI SrfI SwaI