(Linear) (Six-base) MAPSORT of: KIEE5819.seq Check: 899 from: 1 to: 3189 KIEE5819.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. Acc65I G'GTAC_C Cuts at: 0 1794 3189 Size: 1794 1395 AclI AA'CG_TT Cuts at: 0 2447 3189 Size: 2447 742 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 722 992 1028 1596 2206 2759 3189 Size: 184 538 270 36 568 610 553 430 Fragments arranged by size: 610 568 553 538 430 270 184 36 AflII C'TTAA_G Cuts at: 0 2584 3189 Size: 2584 605 AflIII A'CryG_T Cuts at: 0 1788 3189 Size: 1788 1401 AgeI A'CCGG_T Cuts at: 0 661 3189 Size: 661 2528 AhdI GACnn_n'nnGTC Cuts at: 0 2540 3189 Size: 2540 649 AleI CACnn'nnGTG Cuts at: 0 1722 3066 3189 Size: 1722 1344 123 Fragments arranged by size: 1722 1344 123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2144 3189 Size: 2144 1045 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 3019 3051 3189 Size: 97 32 2890 32 138 Fragments arranged by size: 2890 138 97 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 100 162 350 1051 3189 Size: 100 62 188 701 2138 Fragments arranged by size: 2138 701 188 100 62 ApaI G_GGCC'C Cuts at: 0 181 3189 Size: 181 3008 ApoI r'AATT_y Cuts at: 0 1219 3189 Size: 1219 1970 AvaI C'yCGr_G Cuts at: 0 192 220 450 757 1122 2506 3189 Size: 192 28 230 307 365 1384 683 Fragments arranged by size: 1384 683 365 307 230 192 28 BanI G'GyrC_C Cuts at: 0 1794 2077 2779 3150 3189 Size: 1794 283 702 371 39 Fragments arranged by size: 1794 702 371 283 39 BanII G_rGCy'C Cuts at: 0 181 199 230 722 758 1123 1465 3013 Size: 181 18 31 492 36 365 342 1548 Cuts at: 3013 3061 3189 Size: 48 128 Fragments arranged by size: 1548 492 365 342 181 128 48 36 31 18 BbeI G_GCGC'C Cuts at: 0 2081 3189 Size: 2081 1108 BbsI GAAGACnn'nnnn_ Cuts at: 0 880 1016 1191 2697 3189 Size: 880 136 175 1506 492 Fragments arranged by size: 1506 880 492 175 136 BbvCI CC'TCA_GC Cuts at: 0 798 3189 Size: 798 2391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 946 980 2326 2360 3189 Size: 946 34 1346 34 829 Fragments arranged by size: 1346 946 829 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1994 3189 Size: 1994 1195 BfrBI ATG'CAT Cuts at: 0 2048 2993 3189 Size: 2048 945 196 Fragments arranged by size: 2048 945 196 BglI GCCn_nnn'nGGC Cuts at: 0 431 808 3189 Size: 431 377 2381 Fragments arranged by size: 2381 431 377 BlpI GC'TnA_GC Cuts at: 0 827 1117 2251 3012 3189 Size: 827 290 1134 761 177 Fragments arranged by size: 1134 827 761 290 177 Bme1580I G_kGCm'C Cuts at: 0 181 457 3153 3189 Size: 181 276 2696 36 Fragments arranged by size: 2696 276 181 36 BmrI ACTGGGnnnn_n' Cuts at: 0 1656 1837 3189 Size: 1656 181 1352 Fragments arranged by size: 1656 1352 181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 92 2190 3189 Size: 92 2098 999 Fragments arranged by size: 2098 999 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1504 2154 2185 2835 3189 Size: 493 1011 650 31 650 354 Fragments arranged by size: 1011 650 650 493 354 31 Bpu10I CC'TnA_GC Cuts at: 0 798 1464 1984 3088 3189 Size: 798 666 520 1104 101 Fragments arranged by size: 1104 798 666 520 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1000 2425 3189 Size: 1000 1425 764 Fragments arranged by size: 1425 1000 764 BsaI GGTCTCn'nnnn_ Cuts at: 0 934 3118 3189 Size: 934 2184 71 Fragments arranged by size: 2184 934 71 BsaAI yAC'GTr Cuts at: 0 1791 3189 Size: 1791 1398 BsaHI Gr'CG_yC Cuts at: 0 124 2078 3189 Size: 124 1954 1111 Fragments arranged by size: 1954 1111 124 BsaWI w'CCGG_w Cuts at: 0 661 2273 3189 Size: 661 1612 916 Fragments arranged by size: 1612 916 661 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 97 127 472 502 709 739 894 924 Size: 97 30 345 30 207 30 155 30 Cuts at: 924 1124 1154 2503 2533 3019 3049 3189 Size: 200 30 1349 30 486 30 140 Fragments arranged by size: 1349 486 345 207 200 155 140 97 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 899 1145 1223 1226 1863 2524 3027 Size: 238 661 246 78 3 637 661 503 Cuts at: 3027 3111 3147 3189 Size: 84 36 42 Fragments arranged by size: 661 661 637 503 246 238 84 78 42 36 3 BseYI C'CCAG_C Cuts at: 0 413 2073 2871 2942 3083 3108 3189 Size: 413 1660 798 71 141 25 81 Fragments arranged by size: 1660 798 413 141 81 71 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1064 1243 2077 3189 Size: 1064 179 834 1112 Fragments arranged by size: 1112 1064 834 179 BsiHKAI G_wGCw'C Cuts at: 0 722 2339 3013 3189 Size: 722 1617 674 176 Fragments arranged by size: 1617 722 674 176 BsmBI CGTCTCn'nnnn_ Cuts at: 0 952 3189 Size: 952 2237 Bsp1286I G_dGCh'C Cuts at: 0 181 199 230 457 722 758 1123 1465 Size: 181 18 31 227 265 36 365 342 Cuts at: 1465 2339 3013 3061 3153 3189 Size: 874 674 48 92 36 Fragments arranged by size: 874 674 365 342 265 227 181 92 48 36 36 31 18 BspHI T'CATG_A Cuts at: 0 1281 1529 3189 Size: 1281 248 1660 Fragments arranged by size: 1660 1281 248 BsrBI CCG'CTC Cuts at: 0 2161 3189 Size: 2161 1028 BsrDI GCAATG_nn' Cuts at: 0 1798 3180 3189 Size: 1798 1382 9 Fragments arranged by size: 1798 1382 9 BsrFI r'CCGG_y Cuts at: 0 661 2390 3189 Size: 661 1729 799 Fragments arranged by size: 1729 799 661 BstAPI GCAn_nnn'nTGC Cuts at: 0 59 817 1310 2247 2939 3189 Size: 59 758 493 937 692 250 Fragments arranged by size: 937 758 692 493 250 59 BstXI CCAn_nnnn'nTGG Cuts at: 0 421 471 1275 1996 3189 Size: 421 50 804 721 1193 Fragments arranged by size: 1193 804 721 421 50 Bsu36I CC'TnA_GG Cuts at: 0 383 2144 3189 Size: 383 1761 1045 Fragments arranged by size: 1761 1045 383 BtgI C'CryG_G Cuts at: 0 376 2116 2843 3189 Size: 376 1740 727 346 Fragments arranged by size: 1740 727 376 346 BtsI GCAGTG_nn' Cuts at: 0 924 2094 2921 3189 Size: 924 1170 827 268 Fragments arranged by size: 1170 924 827 268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 486 521 3189 Size: 486 35 2668 Fragments arranged by size: 2668 486 35 EaeI y'GGCC_r Cuts at: 0 432 551 2972 3189 Size: 432 119 2421 217 Fragments arranged by size: 2421 432 217 119 EarI CTCTTCn'nnn_ Cuts at: 0 1169 1753 2275 3189 Size: 1169 584 522 914 Fragments arranged by size: 1169 914 584 522 EcoICRI GAG'CTC Cuts at: 0 720 3011 3189 Size: 720 2291 178 Fragments arranged by size: 2291 720 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 493 722 992 1028 1504 1596 2154 Size: 184 309 229 270 36 476 92 558 Cuts at: 2154 2185 2206 2759 2835 3189 Size: 31 21 553 76 354 Fragments arranged by size: 558 553 476 354 309 270 229 184 92 76 36 31 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 649 1567 3166 3189 Size: 649 918 1599 23 Fragments arranged by size: 1599 918 649 23 EcoO109I rG'GnC_Cy Cuts at: 0 177 794 1560 2714 3189 Size: 177 617 766 1154 475 Fragments arranged by size: 1154 766 617 475 177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2734 3189 Size: 2734 455 HaeII r_GCGC'y Cuts at: 0 2081 3182 3189 Size: 2081 1101 7 Fragments arranged by size: 2081 1101 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 472 504 1942 1974 2503 2526 2535 2558 Size: 472 32 1438 32 529 23 9 23 Cuts at: 2558 3043 3075 3189 Size: 485 32 114 Fragments arranged by size: 1438 529 485 472 114 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 0 1428 3189 Size: 1428 1761 KasI G'GCGC_C Cuts at: 0 2077 3189 Size: 2077 1112 KpnI G_GTAC'C Cuts at: 0 1798 3189 Size: 1798 1391 MscI TGG'CCA Cuts at: 0 434 553 2974 3189 Size: 434 119 2421 215 Fragments arranged by size: 2421 434 215 119 MslI CAynn'nnrTG Cuts at: 0 1722 2126 3066 3189 Size: 1722 404 940 123 Fragments arranged by size: 1722 940 404 123 MspA1I CmG'CkG Cuts at: 0 1048 1433 1588 1722 2118 2845 3066 3108 Size: 1048 385 155 134 396 727 221 42 Cuts at: 3108 3189 Size: 81 Fragments arranged by size: 1048 727 396 385 221 155 134 81 42 NarI GG'CG_CC Cuts at: 0 2078 3189 Size: 2078 1111 NcoI C'CATG_G Cuts at: 0 376 3189 Size: 376 2813 NsiI A_TGCA'T Cuts at: 0 2050 2995 3189 Size: 2050 945 194 Fragments arranged by size: 2050 945 194 NspI r_CATG'y Cuts at: 0 25 2766 3189 Size: 25 2741 423 Fragments arranged by size: 2741 423 25 PflMI CCAn_nnn'nTGG Cuts at: 0 534 3189 Size: 534 2655 PfoI T'CCnGG_A Cuts at: 0 244 587 1094 2189 2363 2397 2480 3189 Size: 244 343 507 1095 174 34 83 709 Fragments arranged by size: 1095 709 507 343 244 174 83 34 PmlI CAC'GTG Cuts at: 0 1791 3189 Size: 1791 1398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 1372 1404 3019 3051 3189 Size: 97 32 1243 32 1615 32 138 Fragments arranged by size: 1615 1243 138 97 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1560 2714 3189 Size: 1560 1154 475 Fragments arranged by size: 1560 1154 475 PshAI GACnn'nnGTC Cuts at: 0 1277 3189 Size: 1277 1912 PsiI TTA'TAA Cuts at: 0 1364 3189 Size: 1364 1825 PspOMI G'GGCC_C Cuts at: 0 177 3189 Size: 177 3012 PstI C_TGCA'G Cuts at: 0 55 84 524 773 1625 2569 2629 2664 Size: 55 29 440 249 852 944 60 35 Cuts at: 2664 3189 Size: 525 Fragments arranged by size: 944 852 525 440 249 60 55 35 29 PvuII CAG'CTG Cuts at: 0 1048 1722 3066 3189 Size: 1048 674 1344 123 Fragments arranged by size: 1344 1048 674 123 SacI G_AGCT'C Cuts at: 0 722 3013 3189 Size: 722 2291 176 Fragments arranged by size: 2291 722 176 SacII CC_GC'GG Cuts at: 0 2119 2846 3189 Size: 2119 727 343 Fragments arranged by size: 2119 727 343 SanDI GG'GwC_CC Cuts at: 0 2714 3189 Size: 2714 475 SbfI CC_TGCA'GG Cuts at: 0 524 3189 Size: 524 2665 ScaI AGT'ACT Cuts at: 0 1483 3189 Size: 1483 1706 SexAI A'CCwGG_T Cuts at: 0 1562 2024 2306 3189 Size: 1562 462 282 883 Fragments arranged by size: 1562 883 462 282 SfcI C'TryA_G Cuts at: 0 51 80 520 769 821 1621 2565 2625 Size: 51 29 440 249 52 800 944 60 Cuts at: 2625 2660 3189 Size: 35 529 Fragments arranged by size: 944 800 529 440 249 60 52 51 35 29 SfiI GGCCn_nnn'nGGCC Cuts at: 0 431 3189 Size: 431 2758 SfoI GGC'GCC Cuts at: 0 2079 3189 Size: 2079 1110 SmaI CCC'GGG Cuts at: 0 194 452 759 1124 3189 Size: 194 258 307 365 2065 Fragments arranged by size: 2065 365 307 258 194 SmlI C'TyrA_G Cuts at: 0 1015 2404 2584 3189 Size: 1015 1389 180 605 Fragments arranged by size: 1389 1015 605 180 StuI AGG'CCT Cuts at: 0 644 1868 2515 3189 Size: 644 1224 647 674 Fragments arranged by size: 1224 674 647 644 StyI C'CwwG_G Cuts at: 0 29 376 950 1995 2709 3167 3189 Size: 29 347 574 1045 714 458 22 Fragments arranged by size: 1045 714 574 458 347 29 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1675 1744 3189 Size: 1675 69 1445 Fragments arranged by size: 1675 1445 69 TatI w'GTAC_w Cuts at: 0 1481 1739 3189 Size: 1481 258 1450 Fragments arranged by size: 1481 1450 258 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1124 1156 1915 1947 3189 Size: 1124 32 759 32 1242 Fragments arranged by size: 1242 1124 759 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 468 3189 Size: 468 2721 XmaI C'CCGG_G Cuts at: 0 192 450 757 1122 3189 Size: 192 258 307 365 2067 Fragments arranged by size: 2067 365 307 258 192 XmnI GAAnn'nnTTC Cuts at: 0 1651 2688 3189 Size: 1651 1037 501 Fragments arranged by size: 1651 1037 501 Enzymes that do cut: Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NarI NcoI NsiI NspI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AfeI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BclI BglII BmgBI BmtI BsaBI BsiEI BsiWI BsmI BspEI BspMI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PspXI PsrI PvuI RsrII SalI SapI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI