################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MaeI MaeIII MamI MfeI MluI MluNI MscI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 614 GGTACC G^GTACC AccII 658 CGCG CG^CG AccIII 299 TCCGGA T^CCGGA AcyI 147 GGCGCC G[GA]^CG[CT]C AlwI 564 GGATC GGATC(4/5) AlwNI 121 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 140 GGGCCC GGGCC^C Asp718I 614 GGTACC G^GTACC AspHI 193 GAGCTC G[AT]GC[AT]^C BbeI 147 GGCGCC GGCGC^C BlpI 37 GCTCAGC GC^T[ACGT]AGC BpmI 90 CTCCAG CTGGAG(16/14) Bpu1102I 37 GCTCAGC GC^T[ACGT]AGC BsaHI 147 GGCGCC G[GA]^CG[CT]C BseAI 299 TCCGGA T^CCGGA BsiHKAI 193 GAGCTC G[AT]GC[AT]^C BsmAI 539 GAGAC GTCTC(1/5) BsmBI 539 GAGACG CGTCTC(1/5) BspEI 299 TCCGGA T^CCGGA BspMI 173 ACCTGC ACCTGC(4/8) BstUI 658 CGCG CG^CG BstXI 251 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 564 GGATCT [GA]^GATC[CT] CelII 37 GCTCAGC GC^T[ACGT]AGC DpnII 565 GATC ^GATC DraII 521 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 606 GAAGAG CTCTTC(1/4) EcoO109I 521 AGGACCC [GA]G^G[ACGT]CC[CT] Hin1I 147 GGCGCC G[GA]^CG[CT]C HinfI 226 GACTC G^A[ACGT]TC HphI 626 TCACC GGTGA(8/7) KasI 147 GGCGCC G^GCGCC KpnI 614 GGTACC GGTAC^C Ksp632I 606 GAAGAG CTCTTC(1/4) MaeII 542 ACGT A^CGT MboI 565 GATC ^GATC MflI 564 GGATCT [GA]^GATC[CT] MroI 299 TCCGGA T^CCGGA MseI 764 TTAA T^TAA MvnI 658 CGCG CG^CG NaeI 374 GCCGGC GCC^GGC NarI 147 GGCGCC GG^CGCC NcoI 414 CCATGG C^CATGG NdeII 565 GATC ^GATC NgoMIV 374 GCCGGC G^CCGGC PflMI 120 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 226 GACTC GAGTC(4/5) PpuMI 521 AGGACCC [GA]G^G[AT]CC[CT] PstI 493 CTGCAG CTGCA^G SacI 193 GAGCTC GAGCT^C Sau3AI 565 GATC ^GATC SfaNI 294 GATGC GCATC(5/9) SfcI 493 CTGCAG C^T[GA][CT]AG SfoI 147 GGCGCC GGC^GCC SmaI 143 CCCGGG CCC^GGG TaqI 455 TCGA T^CGA Tru9I 764 TTAA T^TAA TthHB8I 455 TCGA T^CGA Van91I 120 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 716 CCATGAACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 564 GGATCT [GA]^GATC[CT] XmaCI 143 CCCGGG C^CCGGG XmaI 143 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 582 GTAC GT^AC 615 GTAC AvaI 143 CCCGGG C^[CT]CG[GA]G 360 CCCGAG BbsI 463 GAAGAC GAAGAC(2/6) 679 GTCTTC BpuAI 463 GAAGAC GAAGAC(2/6) 679 GTCTTC BsaWI 299 TCCGGA [AT]^CCGG[AT] 722 ACCGGA BsgI 175 CTGCAC GTGCAG(16/14) 340 GTGCAG BsiEI 372 CGGCCG CG[GA][CT]^CG 448 CGGCCG BsmFI 56 GGGAC GGGAC(10/14) 434 GGGAC BsoBI 143 CCCGGG C^[CT]CG[GA]G 360 CCCGAG BsrBI 451 CCGCTC CCGCTC(-3/-3) 136 GAGCGG BsrFI 374 GCCGGC [GA]^CCGG[CT] 395 ACCGGC BssSI 742 CACGAG CACGAG(-5/-1) 473 CTCGTG BstF5I 575 CATCC GGATG(2/0) 725 GGATG BstNI 189 CCAGG CC^[AT]GG 578 CCAGG Cfr10I 374 GCCGGC [GA]^CCGG[CT] 395 ACCGGC DsaI 414 CCATGG C^C[GA][CT]GG 430 CCACGG EaeI 372 CGGCCG [CT]^GGCC[GA] 448 CGGCCG EagI 372 CGGCCG C^GGCCG 448 CGGCCG EclXI 372 CGGCCG C^GGCCG 448 CGGCCG Eco52I 372 CGGCCG C^GGCCG 448 CGGCCG EcoRII 189 CCAGG ^CC[AT]GG 578 CCAGG EcoT14I 414 CCATGG C^C[AT][AT]GG 686 CCAAGG FokI 575 CATCC GGATG(9/13) 725 GGATG MvaI 189 CCAGG CC^[AT]GG 578 CCAGG NlaIII 415 CATG CATG^ 717 CATG RsaI 582 GTAC GT^AC 615 GTAC StyI 414 CCATGG C^C[AT][AT]GG 686 CCAAGG TspRI 346 CACTG CA[GC]TG[ACGT][ACGT]^ 773 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI Cac8I CfoI Cfr13I DdeI FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MwoI NciI NlaIV PvuII Sau96I ScrFI TseI