Restriction map for
pFN21AE5818
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanII BbeI BbrPI BbvCI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BspEI BspLU11I BsrDI BssHII
Bst1107I BstUI BstZ17I CelII CfoI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AclI 756 AACGTT AA^CGTT
AcyI 1123 GGCGTC G[GA]^CG[CT]C
AflII 2557 CTTAAG C^TTAAG
AocI 2736 CCTAAGG CC^T[ACGT]AGG
AvaI 2544 CTCGGG C^[CT]CG[GA]G
BalI 161 TGGCCA TGG^CCA
BamHI 725 GGATCC G^GATCC
BcgI 1499 GCAGACTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2557 CTTAAG C^TTAAG
BglI 648 GCCAATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1123 GGCGTC G[GA]^CG[CT]C
BsaI 2271 GAGACC GGTCTC(1/5)
BsgI 2423 GTGCAG GTGCAG(16/14)
BsmBI 2803 CGTCTC CGTCTC(1/5)
BsoBI 2544 CTCGGG C^[CT]CG[GA]G
Bsp1407I 371 TGTACA T^GTACA
BspDI 604 ATCGAT AT^CGAT
BsrBI 1438 GAGCGG CCGCTC(-3/-3)
BsrFI 1072 ACCGGC [GA]^CCGG[CT]
BsrGI 371 TGTACA T^GTACA
BssSI 2680 CTCGTG CACGAG(-5/-1)
Bsu36I 2736 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1072 ACCGGC [GA]^CCGG[CT]
ClaI 604 ATCGAT AT^CGAT
Eco81I 2736 CCTAAGG CC^T[ACGT]AGG
EcoRV 1177 GATATC GAT^ATC
EcoT22I 2354 ATGCAT ATGCA^T
HgaI 1124 GCGTC GACGC(5/10)
Hin1I 1123 GGCGTC G[GA]^CG[CT]C
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
NsiI 2354 ATGCAT ATGCA^T
Psp1406I 756 AACGTT AA^CGTT
SpeI 253 ACTAGT A^CTAGT
SspBI 371 TGTACA T^GTACA
XcmI 2430 CCAAGGAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 1652 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1751 CAGAGTCTG
AspHI 1699 GTGCTC G[AT]GC[AT]^C
2674 GAGCAC
AvaII 2053 GGACC G^G[AT]CC
2416 GGACC
BanI 1867 GGTGCC G^G[CT][GA]CC
2604 GGTGCC
BbsI 43 GTCTTC GAAGAC(2/6)
1282 GTCTTC
BpuAI 43 GTCTTC GAAGAC(2/6)
1282 GTCTTC
BsiHKAI 1699 GTGCTC G[AT]GC[AT]^C
2674 GAGCAC
BspHI 593 TCATGA T^CATGA
1217 TCATGA
BspMI 572 ACCTGC ACCTGC(4/8)
620 GCAGGT
BstAPI 206 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1348 GCAGTAATTGC
BstBI 923 TTCGAA TT^CGAA
2489 TTCGAA
BstEII 635 GGTTACC G^GT[ACGT]ACC
1251 GGTAACC
BstPI 635 GGTTACC G^GT[ACGT]ACC
1251 GGTAACC
BstXI 272 CCAGCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCACCCTAGTGG
BstYI 725 GGATCC [GA]^GATC[CT]
1008 GGATCT
DraIII 2259 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2677 CACCTCGTG
DsaI 502 CCATGG C^C[GA][CT]GG
930 CCATGG
EaeI 161 TGGCCA [CT]^GGCC[GA]
2860 TGGCCG
EcoO65I 635 GGTTACC G^GT[ACGT]ACC
1251 GGTAACC
EcoRI 2315 GAATTC G^AATTC
2333 GAATTC
FauI 578 CCCGC CCCGC(4/6)
2589 CCCGC
MaeII 757 ACGT A^CGT
2802 ACGT
MflI 725 GGATCC [GA]^GATC[CT]
1008 GGATCT
NcoI 502 CCATGG C^CATGG
930 CCATGG
NspV 923 TTCGAA TT^CGAA
2489 TTCGAA
PflMI 999 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCAATTTCTGG
PstI 2565 CTGCAG CTGCA^G
2623 CTGCAG
RcaI 593 TCATGA T^CATGA
1217 TCATGA
SapI 11 GCTCTTC GCTCTTC(1/4)
1080 GCTCTTC
SfuI 923 TTCGAA TT^CGAA
2489 TTCGAA
SmlI 1875 CTTGAG C^T[CT][GA]AG
2557 CTTAAG
SphI 495 GCATGC GCATG^C
1076 GCATGC
SspI 175 AATATT AAT^ATT
1524 AATATT
Van91I 999 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCAATTTCTGG
XhoII 725 GGATCC [GA]^GATC[CT]
1008 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI