(Circular) (Six-base) MAPSORT of: pFN21AE5818.seq Check: 5814 from: 1 to: 7570 pFN21AE5818 7570 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5380 278 Size: 53 83 186 936 3844 2468 Fragments arranged by size: 3844 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4913 1937 Size: 2976 4594 Acc65I G'GTAC_C Cuts at: 4891 4891 Size: 7570 AclI AA'CG_TT Cuts at: 1563 2762 5697 6070 1563 Size: 1199 2935 373 3063 Fragments arranged by size: 3063 2935 1199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 4091 4142 5633 7017 7285 1632 Size: 618 1841 51 1491 1384 268 1917 Fragments arranged by size: 1917 1841 1491 1384 618 268 51 AflII C'TTAA_G Cuts at: 820 1017 4562 820 Size: 197 3545 3828 Fragments arranged by size: 3828 3545 197 AflIII A'CryG_T Cuts at: 1203 6469 7446 1203 Size: 5266 977 1327 Fragments arranged by size: 5266 1327 977 AgeI A'CCGG_T Cuts at: 6401 6401 Size: 7570 AhdI GACnn_n'nnGTC Cuts at: 6299 6299 Size: 7570 AleI CACnn'nnGTG Cuts at: 1166 3870 3921 1166 Size: 2704 51 4815 Fragments arranged by size: 4815 2704 51 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7570 AlwNI CAG_nnn'CTG Cuts at: 3662 3761 5313 6885 3662 Size: 99 1552 1572 4347 Fragments arranged by size: 4347 1572 1552 99 ApaLI G'TGCA_C Cuts at: 5627 6783 5627 Size: 1156 6414 ApoI r'AATT_y Cuts at: 1493 3736 4236 4256 4308 4320 4338 4497 4879 Size: 2243 500 20 52 12 18 159 382 Cuts at: 4879 5105 5257 7366 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 500 382 226 159 152 52 20 18 12 AseI AT'TA_AT Cuts at: 160 1032 6124 160 Size: 872 5092 1606 Fragments arranged by size: 5092 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 AvaI C'yCGr_G Cuts at: 1870 1943 4549 4895 1870 Size: 73 2606 346 4545 Fragments arranged by size: 4545 2606 346 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3667 3700 3667 Size: 33 7537 BamHI G'GATC_C Cuts at: 2730 4900 5267 2730 Size: 2170 367 5033 Fragments arranged by size: 5033 2170 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3872 4609 4891 6346 618 Size: 325 217 627 2085 737 282 1455 1842 Fragments arranged by size: 2085 1842 1455 737 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 4889 1391 Size: 3498 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 3280 928 Size: 971 142 1239 5218 Fragments arranged by size: 5218 1239 971 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3493 3527 5761 5795 1903 Size: 34 1556 34 2234 34 3678 Fragments arranged by size: 3678 2234 1556 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5462 6672 5462 Size: 1210 6360 BclI T'GATC_A Cuts at: 1548 1548 Size: 7570 BfrBI ATG'CAT Cuts at: 4361 4361 Size: 7570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2659 6181 Size: 107 122 71 631 605 337 650 3522 Cuts at: 6181 136 Size: 1525 Fragments arranged by size: 3522 1525 650 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7565 7565 Size: 7570 BlpI GC'TnA_GC Cuts at: 4987 4987 Size: 7570 Bme1580I G_kGCm'C Cuts at: 1790 4614 5631 6787 1790 Size: 2824 1017 1156 2573 Fragments arranged by size: 2824 2573 1156 1017 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3379 3394 3409 Size: 416 53 83 399 24 1962 15 15 Cuts at: 3409 5301 6259 442 Size: 1892 958 1753 Fragments arranged by size: 1962 1892 1753 958 416 399 83 53 24 15 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7570 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 7570 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 3350 4796 6230 1239 Size: 1469 642 1446 1434 2579 Fragments arranged by size: 2579 1469 1446 1434 642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 5695 6560 6858 3901 Size: 1794 865 298 4613 Fragments arranged by size: 4613 1794 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4270 6233 882 Size: 617 2771 1963 2219 Fragments arranged by size: 2771 2219 1963 617 BsaAI yAC'GTr Cuts at: 493 1204 5361 493 Size: 711 4157 2702 Fragments arranged by size: 4157 2702 711 BsaBI GATnn'nnATC Cuts at: 7564 7564 Size: 7570 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3129 5377 5759 6394 Size: 53 83 186 936 1596 2248 382 635 Cuts at: 6394 275 Size: 1451 Fragments arranged by size: 2248 1596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6003 6401 6675 6822 7233 6003 Size: 398 274 147 411 6340 Fragments arranged by size: 6340 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 2444 2474 3270 Size: 30 165 30 886 30 125 30 796 Cuts at: 3270 3300 4772 4802 1178 Size: 30 1472 30 3946 Fragments arranged by size: 3946 1472 886 796 165 125 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 3410 4288 4548 1186 Size: 1519 705 878 260 4208 Fragments arranged by size: 4208 1519 878 705 260 BseYI C'CCAG_C Cuts at: 2973 3091 4445 6773 7269 2973 Size: 118 1354 2328 496 3274 Fragments arranged by size: 3274 2328 1354 496 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4449 1163 Size: 573 2713 4284 Fragments arranged by size: 4284 2713 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5020 5781 6809 1540 Size: 275 186 3019 761 1028 2301 Fragments arranged by size: 3019 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3708 4683 5631 5716 6787 3708 Size: 975 948 85 1071 4491 Fragments arranged by size: 4491 1071 975 948 85 BsmI GAATG_Cn' Cuts at: 2556 3839 4123 4480 5081 5174 2556 Size: 1283 284 357 601 93 4952 Fragments arranged by size: 4952 1283 601 357 284 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4814 4814 Size: 7570 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3708 4614 4683 4889 5631 5716 6787 Size: 399 1918 906 69 206 742 85 1071 Cuts at: 6787 1391 Size: 2174 Fragments arranged by size: 2174 1918 1071 906 742 399 206 85 69 BspEI T'CCGG_A Cuts at: 7233 7233 Size: 7570 BspHI T'CATG_A Cuts at: 2598 3222 5459 7189 2598 Size: 624 2237 1730 2979 Fragments arranged by size: 2979 2237 1730 624 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 2616 4925 844 Size: 1742 30 2309 3489 Fragments arranged by size: 3489 2309 1742 30 BsrBI CCG'CTC Cuts at: 1394 1823 3445 5457 1394 Size: 429 1622 2012 3507 Fragments arranged by size: 3507 2012 1622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6065 6239 65 Size: 1183 4817 174 1396 Fragments arranged by size: 4817 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3077 4867 6214 6401 1700 Size: 110 1267 1790 1347 187 2869 Fragments arranged by size: 2869 1790 1347 1267 187 110 BsrGI T'GTAC_A Cuts at: 96 2376 96 Size: 2280 5290 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7570 BssSI C'ACGA_G Cuts at: 4685 5630 6642 4685 Size: 945 1012 5613 Fragments arranged by size: 5613 1012 945 BstAPI GCAn_nnn'nTGC Cuts at: 794 2217 3359 794 Size: 1423 1142 5005 Fragments arranged by size: 5005 1423 1142 BstBI TT'CG_AA Cuts at: 2929 4495 6374 2929 Size: 1566 1879 4125 Fragments arranged by size: 4125 1879 1566 BstEII G'GTnAC_C Cuts at: 1184 2640 3256 1184 Size: 1456 616 5498 Fragments arranged by size: 5498 1456 616 BstXI CCAn_nnnn'nTGG Cuts at: 2284 3923 2284 Size: 1639 5931 BstYI r'GATC_y Cuts at: 1972 2730 3013 4900 5267 5652 5669 7121 7207 Size: 758 283 1887 367 385 17 1452 86 Cuts at: 7207 7219 7565 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1887 1452 758 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4742 4742 Size: 7570 BtgI C'CryG_G Cuts at: 513 1065 2004 2507 2935 513 Size: 552 939 503 428 5148 Fragments arranged by size: 5148 939 552 503 428 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3820 487 Size: 683 56 2594 4237 Fragments arranged by size: 4237 2594 683 56 BtsI GCAGTG_nn' Cuts at: 2675 2706 4546 4625 5086 5880 5900 2675 Size: 31 1840 79 461 794 20 4345 Fragments arranged by size: 4345 1840 794 461 79 31 20 ClaI AT'CG_AT Cuts at: 1554 2610 1554 Size: 1056 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7535 DraI TTT'AAA Cuts at: 4874 4874 Size: 7570 DraIII CAC_nnn'GTG Cuts at: 4269 4687 4269 Size: 418 7152 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6577 809 Size: 507 5261 1802 Fragments arranged by size: 5261 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 4865 5017 Size: 54 1425 50 275 18 336 2699 152 Cuts at: 5017 5906 7535 8 Size: 889 1629 43 Fragments arranged by size: 2699 1629 1425 889 336 275 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5017 1537 Size: 275 3205 4090 Fragments arranged by size: 4090 3205 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2023 2241 3092 3387 3903 4136 4753 5500 Size: 670 218 851 295 516 233 617 747 Cuts at: 5500 1353 Size: 3423 Fragments arranged by size: 3423 851 747 670 617 516 295 233 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6157 6531 6677 387 Size: 1064 138 4568 374 146 1280 Fragments arranged by size: 4568 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2708 3350 4091 4142 4796 5633 Size: 393 618 458 642 741 51 654 837 Cuts at: 5633 6230 7017 7285 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 837 787 741 654 642 618 597 458 393 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 2897 3880 4428 2897 Size: 983 548 6039 Fragments arranged by size: 6039 983 548 EcoRI G'AATT_C Cuts at: 4320 4338 4879 5257 4320 Size: 18 541 378 6633 Fragments arranged by size: 6633 541 378 18 EcoRV GAT'ATC Cuts at: 3184 3184 Size: 7570 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2571 2571 Size: 7570 FspI TGC'GCA Cuts at: 6076 6076 Size: 7570 HaeII r_GCGC'y Cuts at: 1678 6717 1678 Size: 5039 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2946 2978 3099 Size: 32 135 32 872 32 470 32 121 Cuts at: 3099 3131 3270 3302 4502 4514 4534 4546 6211 Size: 32 139 32 1200 12 20 12 1665 Cuts at: 6211 6243 6285 6317 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1665 1200 872 470 139 135 121 42 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4914 5160 669 Size: 988 281 2976 246 3079 Fragments arranged by size: 3079 2976 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7570 HpaI GTT'AAC Cuts at: 5160 5160 Size: 7570 KpnI G_GTAC'C Cuts at: 4895 4895 Size: 7570 MfeI C'AATT_G Cuts at: 5169 5169 Size: 7570 MluI A'CGCG_T Cuts at: 7446 7446 Size: 7570 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2412 6684 6868 1305 Size: 1107 4272 184 2007 Fragments arranged by size: 4272 2007 1107 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 5412 Fragments arranged by size: 5412 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 3080 3870 3921 5528 5887 6046 Size: 648 1206 708 790 51 1607 359 159 Cuts at: 6046 518 Size: 2042 Fragments arranged by size: 2042 1607 1206 790 708 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2089 3095 3240 4986 5663 6811 7056 1606 Size: 483 1006 145 1746 677 1148 245 2120 Fragments arranged by size: 2120 1746 1148 1006 677 483 245 145 NcoI C'CATG_G Cuts at: 513 1065 2004 2507 2935 513 Size: 552 939 503 428 5148 Fragments arranged by size: 5148 939 552 503 428 NdeI CA'TA_TG Cuts at: 387 387 Size: 7570 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7570 NotI GC'GGCC_GC Cuts at: 5017 5017 Size: 7570 NsiI A_TGCA'T Cuts at: 4363 4363 Size: 7570 NspI r_CATG'y Cuts at: 1132 1229 2504 3085 4643 4928 6473 1132 Size: 97 1275 581 1558 285 1545 2229 Fragments arranged by size: 2229 1558 1545 1275 581 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7570 PciI A'CATG_T Cuts at: 6469 6469 Size: 7570 PflMI CCAn_nnn'nTGG Cuts at: 1108 3010 3461 1108 Size: 1902 451 5217 Fragments arranged by size: 5217 1902 451 PfoI T'CCnGG_A Cuts at: 3327 3327 Size: 7570 PmeI GTTT'AAAC Cuts at: 4874 4874 Size: 7570 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5639 5671 7152 7184 5639 Size: 32 1481 32 6025 Fragments arranged by size: 6025 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7570 PsiI TTA'TAA Cuts at: 3962 4413 5140 3962 Size: 451 727 6392 Fragments arranged by size: 6392 727 451 PstI C_TGCA'G Cuts at: 830 4574 4632 4922 830 Size: 3744 58 290 3478 Fragments arranged by size: 3744 3478 290 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7570 PvuII CAG'CTG Cuts at: 2089 3095 3240 2089 Size: 1006 145 6419 Fragments arranged by size: 6419 1006 145 SalI G'TCGA_C Cuts at: 1936 4912 1936 Size: 2976 4594 SapI GCTCTTCn'nnn_ Cuts at: 2023 3092 2023 Size: 1069 6501 SbfI CC_TGCA'GG Cuts at: 4922 4922 Size: 7570 ScaI AGT'ACT Cuts at: 2263 4568 4769 5818 7530 2263 Size: 2305 201 1049 1712 2303 Fragments arranged by size: 2305 2303 1712 1049 201 SfcI C'TryA_G Cuts at: 826 1045 3177 4570 4628 4918 6053 6734 6925 Size: 219 2132 1393 58 290 1135 681 191 Cuts at: 6925 826 Size: 1471 Fragments arranged by size: 2132 1471 1393 1135 681 290 219 191 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7570 SmaI CCC'GGG Cuts at: 1872 4897 1872 Size: 3025 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4562 5674 6575 6837 820 Size: 197 926 1937 682 1112 901 262 1553 Fragments arranged by size: 1937 1553 1112 926 901 682 262 197 SnaBI TAC'GTA Cuts at: 493 5361 493 Size: 4868 2702 SpeI A'CTAG_T Cuts at: 152 2258 152 Size: 2106 5464 SphI G_CATG'C Cuts at: 1132 2504 3085 4928 1132 Size: 1372 581 1843 3774 Fragments arranged by size: 3774 1843 1372 581 SspI AAT'ATT Cuts at: 5 52 2182 3531 5494 5 Size: 47 2130 1349 1963 2081 Fragments arranged by size: 2130 2081 1963 1349 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7570 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2507 2518 2935 3252 4435 Size: 552 939 165 338 11 417 317 1183 Cuts at: 4435 5009 513 Size: 574 3074 Fragments arranged by size: 3074 1183 939 574 552 417 338 317 165 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2070 3882 5597 5614 5767 5952 6397 Size: 1255 490 1812 1715 17 153 185 445 Cuts at: 6397 325 Size: 1498 Fragments arranged by size: 1812 1715 1498 1255 490 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2261 2376 4566 Size: 274 80 33 51 1443 284 115 2190 Cuts at: 4566 4767 5816 7528 96 Size: 201 1049 1712 138 Fragments arranged by size: 2190 1712 1443 1049 284 274 201 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2289 2321 7530 7562 2289 Size: 32 5209 32 2297 Fragments arranged by size: 5209 2297 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7570 XbaI T'CTAG_A Cuts at: 4906 4906 Size: 7570 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4442 4442 Size: 7570 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7570 XmaI C'CCGG_G Cuts at: 1870 4895 1870 Size: 3025 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3989 4152 5699 3776 Size: 213 163 1547 5647 Fragments arranged by size: 5647 1547 213 163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5378 276 Size: 53 83 186 936 3844 2468 Fragments arranged by size: 3844 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI