(Linear) (Six-base) MAPSORT of: KIEE5818.seq Check: 8490 from: 1 to: 2865 KIEE5818.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AclI AA'CG_TT Cuts at: 0 757 2865 Size: 757 2108 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 2086 2137 2865 Size: 245 1841 51 728 Fragments arranged by size: 1841 728 245 51 AflII C'TTAA_G Cuts at: 0 2557 2865 Size: 2557 308 AleI CACnn'nnGTG Cuts at: 0 1865 1916 2865 Size: 1865 51 949 Fragments arranged by size: 1865 949 51 AlwNI CAG_nnn'CTG Cuts at: 0 1657 1756 2865 Size: 1657 99 1109 Fragments arranged by size: 1657 1109 99 ApoI r'AATT_y Cuts at: 0 1731 2231 2251 2303 2315 2333 2492 2865 Size: 1731 500 20 52 12 18 159 373 Fragments arranged by size: 1731 500 373 159 52 20 18 12 AvaI C'yCGr_G Cuts at: 0 2544 2865 Size: 2544 321 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1662 1695 2865 Size: 1662 33 1170 Fragments arranged by size: 1662 1170 33 BamHI G'GATC_C Cuts at: 0 725 2865 Size: 725 2140 BanI G'GyrC_C Cuts at: 0 1867 2604 2865 Size: 1867 737 261 Fragments arranged by size: 1867 737 261 BbsI GAAGACnn'nnnn_ Cuts at: 0 36 1275 2865 Size: 36 1239 1590 Fragments arranged by size: 1590 1239 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1488 1522 2865 Size: 1488 34 1343 Fragments arranged by size: 1488 1343 34 BfrBI ATG'CAT Cuts at: 0 2356 2865 Size: 2356 509 BglI GCCn_nnn'nGGC Cuts at: 0 654 2865 Size: 654 2211 Bme1580I G_kGCm'C Cuts at: 0 2609 2865 Size: 2609 256 BmrI ACTGGGnnnn_n' Cuts at: 0 1374 1389 1404 2865 Size: 1374 15 15 1461 Fragments arranged by size: 1461 1374 15 15 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 27 2865 Size: 27 2838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1345 2791 2865 Size: 703 642 1446 74 Fragments arranged by size: 1446 703 642 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1896 2865 Size: 1896 969 BsaI GGTCTCn'nnnn_ Cuts at: 0 2265 2865 Size: 2265 600 BsaHI Gr'CG_yC Cuts at: 0 1124 2865 Size: 1124 1741 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 284 314 439 469 1265 1295 2767 2797 Size: 284 30 125 30 796 30 1472 30 Cuts at: 2797 2865 Size: 68 Fragments arranged by size: 1472 796 284 125 68 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 700 1405 2283 2543 2865 Size: 700 705 878 260 322 Fragments arranged by size: 878 705 700 322 260 BseYI C'CCAG_C Cuts at: 0 968 1086 2440 2865 Size: 968 118 1354 425 Fragments arranged by size: 1354 968 425 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2444 2865 Size: 2444 421 BsiHKAI G_wGCw'C Cuts at: 0 1703 2678 2865 Size: 1703 975 187 Fragments arranged by size: 1703 975 187 BsmI GAATG_Cn' Cuts at: 0 551 1834 2118 2475 2865 Size: 551 1283 284 357 390 Fragments arranged by size: 1283 551 390 357 284 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2809 2865 Size: 2809 56 Bsp1286I G_dGCh'C Cuts at: 0 1703 2609 2678 2865 Size: 1703 906 69 187 Fragments arranged by size: 1703 906 187 69 BspHI T'CATG_A Cuts at: 0 593 1217 2865 Size: 593 624 1648 Fragments arranged by size: 1648 624 593 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 581 611 2865 Size: 581 30 2254 Fragments arranged by size: 2254 581 30 BsrBI CCG'CTC Cuts at: 0 1440 2865 Size: 1440 1425 BsrFI r'CCGG_y Cuts at: 0 1072 2865 Size: 1072 1793 BsrGI T'GTAC_A Cuts at: 0 371 2865 Size: 371 2494 BssSI C'ACGA_G Cuts at: 0 2680 2865 Size: 2680 185 BstAPI GCAn_nnn'nTGC Cuts at: 0 212 1354 2865 Size: 212 1142 1511 Fragments arranged by size: 1511 1142 212 BstBI TT'CG_AA Cuts at: 0 924 2490 2865 Size: 924 1566 375 Fragments arranged by size: 1566 924 375 BstEII G'GTnAC_C Cuts at: 0 635 1251 2865 Size: 635 616 1614 Fragments arranged by size: 1614 635 616 BstXI CCAn_nnnn'nTGG Cuts at: 0 279 1918 2865 Size: 279 1639 947 Fragments arranged by size: 1639 947 279 BstYI r'GATC_y Cuts at: 0 725 1008 2865 Size: 725 283 1857 Fragments arranged by size: 1857 725 283 Bsu36I CC'TnA_GG Cuts at: 0 2737 2865 Size: 2737 128 BtgI C'CryG_G Cuts at: 0 502 930 2865 Size: 502 428 1935 Fragments arranged by size: 1935 502 428 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1815 2865 Size: 1815 1050 BtsI GCAGTG_nn' Cuts at: 0 670 701 2541 2620 2865 Size: 670 31 1840 79 245 Fragments arranged by size: 1840 670 245 79 31 ClaI AT'CG_AT Cuts at: 0 605 2865 Size: 605 2260 DraIII CAC_nnn'GTG Cuts at: 0 2264 2682 2865 Size: 2264 418 183 Fragments arranged by size: 2264 418 183 EaeI y'GGCC_r Cuts at: 0 161 2860 2865 Size: 161 2699 5 Fragments arranged by size: 2699 161 5 EarI CTCTTCn'nnn_ Cuts at: 0 18 236 1087 1382 1898 2131 2748 2865 Size: 18 218 851 295 516 233 617 117 Fragments arranged by size: 851 617 516 295 233 218 117 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 703 1345 2086 2137 2791 2865 Size: 245 458 642 741 51 654 74 Fragments arranged by size: 741 654 642 458 245 74 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 892 1875 2423 2865 Size: 892 983 548 442 Fragments arranged by size: 983 892 548 442 EcoRI G'AATT_C Cuts at: 0 2315 2333 2865 Size: 2315 18 532 Fragments arranged by size: 2315 532 18 EcoRV GAT'ATC Cuts at: 0 1179 2865 Size: 1179 1686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 566 2865 Size: 566 2299 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 439 471 941 973 1094 1126 1265 1297 Size: 439 32 470 32 121 32 139 32 Cuts at: 1297 2497 2509 2529 2541 2865 Size: 1200 12 20 12 324 Fragments arranged by size: 1200 470 439 324 139 121 32 32 32 32 20 12 12 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 407 2865 Size: 407 2458 MscI TGG'CCA Cuts at: 0 163 2865 Size: 163 2702 MslI CAynn'nnrTG Cuts at: 0 367 1075 1865 1916 2865 Size: 367 708 790 51 949 Fragments arranged by size: 949 790 708 367 51 MspA1I CmG'CkG Cuts at: 0 84 1090 1235 2865 Size: 84 1006 145 1630 Fragments arranged by size: 1630 1006 145 84 NcoI C'CATG_G Cuts at: 0 502 930 2865 Size: 502 428 1935 Fragments arranged by size: 1935 502 428 NsiI A_TGCA'T Cuts at: 0 2358 2865 Size: 2358 507 NspI r_CATG'y Cuts at: 0 499 1080 2638 2865 Size: 499 581 1558 227 Fragments arranged by size: 1558 581 499 227 PflMI CCAn_nnn'nTGG Cuts at: 0 1005 1456 2865 Size: 1005 451 1409 Fragments arranged by size: 1409 1005 451 PfoI T'CCnGG_A Cuts at: 0 1322 2865 Size: 1322 1543 PsiI TTA'TAA Cuts at: 0 1957 2408 2865 Size: 1957 451 457 Fragments arranged by size: 1957 457 451 PstI C_TGCA'G Cuts at: 0 2569 2627 2865 Size: 2569 58 238 Fragments arranged by size: 2569 238 58 PvuII CAG'CTG Cuts at: 0 84 1090 1235 2865 Size: 84 1006 145 1630 Fragments arranged by size: 1630 1006 145 84 SapI GCTCTTCn'nnn_ Cuts at: 0 18 1087 2865 Size: 18 1069 1778 Fragments arranged by size: 1778 1069 18 ScaI AGT'ACT Cuts at: 0 258 2563 2764 2865 Size: 258 2305 201 101 Fragments arranged by size: 2305 258 201 101 SfcI C'TryA_G Cuts at: 0 1172 2565 2623 2865 Size: 1172 1393 58 242 Fragments arranged by size: 1393 1172 242 58 SmlI C'TyrA_G Cuts at: 0 1875 2557 2865 Size: 1875 682 308 Fragments arranged by size: 1875 682 308 SpeI A'CTAG_T Cuts at: 0 253 2865 Size: 253 2612 SphI G_CATG'C Cuts at: 0 499 1080 2865 Size: 499 581 1785 Fragments arranged by size: 1785 581 499 SspI AAT'ATT Cuts at: 0 177 1526 2865 Size: 177 1349 1339 Fragments arranged by size: 1349 1339 177 StyI C'CwwG_G Cuts at: 0 164 502 513 930 1247 2430 2865 Size: 164 338 11 417 317 1183 435 Fragments arranged by size: 1183 435 417 338 317 164 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 65 1877 2865 Size: 65 1812 988 Fragments arranged by size: 1812 988 65 TatI w'GTAC_w Cuts at: 0 256 371 2561 2762 2865 Size: 256 115 2190 201 103 Fragments arranged by size: 2190 256 201 115 103 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 284 316 2865 Size: 284 32 2549 Fragments arranged by size: 2549 284 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2437 2865 Size: 2437 428 XmnI GAAnn'nnTTC Cuts at: 0 1771 1984 2147 2865 Size: 1771 213 163 718 Fragments arranged by size: 1771 718 213 163 Enzymes that do cut: AclI AcuI AflII AleI AlwNI ApoI AvaI BaeI BamHI BanI BbsI BcgI BfrBI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI PsiI PstI PvuII SapI ScaI SfcI SmlI SpeI SphI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BanII BbeI BbvCI BciVI BclI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrDI BssHII BstZ17I CspCI DraI DrdI EagI EciI EcoICRI EcoO109I FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI