################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 430 CGCG CG^CG AspHI 328 GAGCAC G[AT]GC[AT]^C BciVI 274 GTATCC GTATCC(6/5) BglII 658 AGATCT A^GATCT BpmI 686 CTGGAG CTGGAG(16/14) BsgI 541 CTGCAC GTGCAG(16/14) BsiEI 596 CGGCCG CG[GA][CT]^CG BsiHKAI 328 GAGCAC G[AT]GC[AT]^C BsmAI 41 GAGAC GTCTC(1/5) BsmFI 149 GGGAC GGGAC(10/14) BsmI 535 GCATTC GAATGC(1/-1) Bsp1407I 482 TGTACA T^GTACA BsrGI 482 TGTACA T^GTACA BsrI 377 ACTGG ACTGG(1/-1) BstAPI 498 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 430 CGCG CG^CG BstYI 658 AGATCT [GA]^GATC[CT] DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 596 CGGCCG C^GGCCG EclXI 596 CGGCCG C^GGCCG Eco52I 596 CGGCCG C^GGCCG EcoRI 316 GAATTC G^AATTC EcoT14I 508 CCTTGG C^C[AT][AT]GG EcoT22I 533 ATGCAT ATGCA^T HaeII 399 GGCGCT [GA]GCGC^[CT] MflI 658 AGATCT [GA]^GATC[CT] MslI 331 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 235 CAGCTG C[AC]G^C[GT]G MvnI 430 CGCG CG^CG NsiI 533 ATGCAT ATGCA^T PleI 414 GAGTC GAGTC(4/5) PstI 405 CTGCAG CTGCA^G PvuII 235 CAGCTG CAG^CTG SspBI 482 TGTACA T^GTACA StyI 508 CCTTGG C^C[AT][AT]GG XhoII 658 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 425 GAGCCC G[GA]GC[CT]^C 689 GAGCCC BbvI 237 GCTGC GCAGC(8/12) 611 GCTGC BsiYI 285 CCCCTGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 599 CCGCCATGCGG BslI 285 CCCCTGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 599 CCGCCATGCGG BsrBI 132 CCGCTC CCGCTC(-3/-3) 28 GAGCGG BstF5I 75 CATCC GGATG(2/0) 32 GGATG CfoI 400 GCGC GCG^C 614 GCGC EaeI 311 TGGCCG [CT]^GGCC[GA] 596 CGGCCG FauI 362 CCCGC CCCGC(4/6) 428 CCCGC FokI 75 CATCC GGATG(9/13) 32 GGATG HhaI 400 GCGC GCG^C 614 GCGC HinP1I 400 GCGC G^CGC 614 GCGC HphI 263 TCACC GGTGA(8/7) 341 TCACC MaeIII 340 GTCAC ^GT[ACGT]AC 669 GTCAC SexAI 265 ACCTGGT A^CC[AT]GGT 625 ACCTGGT SfcI 405 CTGCAG C^T[GA][CT]AG 662 CTACAG TseI 237 GCTGC G^C[AT]GC 611 GCTGC Tsp45I 340 GTCAC ^GT[GC]AC 669 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BcnI BmyI BsaJI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tsp509I TthHB8I