Restriction map for
pFN21AE6651
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# NO CUT ENZYMES ( 161 enzymes) #
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Acc65I AccIII AfaI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaII
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FokI
FseI FspI HaeII HgaI HindIII
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RcaI RsaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 155 GACGTC GACGT^C
AccI 176 GTCGAC GT^[AC][GT]AC
AclI 295 AACGTT AA^CGTT
AcyI 155 GACGTC G[GA]^CG[CT]C
AhdI 664 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 664 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 67 CCCGGG C^[CT]CG[GA]G
BamHI 635 GGATCC G^GATCC
BanI 56 GGTGCC G^G[CT][GA]CC
BclI 705 TGATCA T^GATCA
BglII 595 AGATCT A^GATCT
BmyI 43 GGGCAC G[AGT]GC[ACT]^C
BsaHI 155 GACGTC G[GA]^CG[CT]C
BsaI 616 GAGACC GGTCTC(1/5)
BsaJI 67 CCCGGG C^C[ACGT][ACGT]GG
BsaWI 698 ACCGGA [AT]^CCGG[AT]
BsmBI 157 CGTCTC CGTCTC(1/5)
BsmFI 405 GGGAC GGGAC(10/14)
BsoBI 67 CCCGGG C^[CT]CG[GA]G
Bsp1286I 43 GGGCAC G[AGT]GC[ACT]^C
BsrFI 59 GCCGGT [GA]^CCGG[CT]
BsrI 402 ACTGG ACTGG(1/-1)
BstXI 219 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 79 GCGC GCG^C
Cfr10I 59 GCCGGT [GA]^CCGG[CT]
Eam1105I 664 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 163 CCCGC CCCGC(4/6)
FbaI 705 TGATCA T^GATCA
HhaI 79 GCGC GCG^C
Hin1I 155 GACGTC G[GA]^CG[CT]C
HinP1I 79 GCGC G^CGC
HpaI 292 GTTAAC GTT^AAC
MaeIII 428 GTCAC ^GT[ACGT]AC
MseI 293 TTAA T^TAA
MslI 430 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 333 GAGTC GAGTC(4/5)
Psp1406I 295 AACGTT AA^CGTT
SalI 176 GTCGAC G^TCGAC
SmaI 67 CCCGGG CCC^GGG
SmlI 409 CTTGAG C^T[CT][GA]AG
TaqI 177 TCGA T^CGA
TfiI 444 GATTC G^A[AT]TC
Tru9I 293 TTAA T^TAA
Tsp45I 428 GTCAC ^GT[GC]AC
TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 177 TCGA T^CGA
XmaCI 67 CCCGGG C^CCGGG
XmaI 67 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 27 CGCG CG^CG
165 CGCG
AcsI 414 GAATTC [GA]^AATT[CT]
686 GAATTC
ApoI 414 GAATTC [GA]^AATT[CT]
686 GAATTC
BcnI 67 CCCGG CC^[GC]GG
68 CCGGG
BseRI 103 GAGGAG GAGGAG(10/8)
114 GAGGAG
BssKI 67 CCCGG ^CC[ACGT]GG
68 CCGGG
BstUI 27 CGCG CG^CG
165 CGCG
Cfr13I 65 GGCCC G^G[ACGT]CC
70 GGGCC
EarI 326 CTCTTC CTCTTC(1/4)
544 GAAGAG
EcoRI 414 GAATTC G^AATTC
686 GAATTC
HincII 176 GTCGAC GT[CT]^[GA]AC
292 GTTAAC
HindII 176 GTCGAC GT[CT]^[GA]AC
292 GTTAAC
HinfI 333 GAGTC G^A[ACGT]TC
444 GATTC
HphI 361 GGTGA GGTGA(8/7)
347 TCACC
Ksp632I 326 CTCTTC CTCTTC(1/4)
544 GAAGAG
MspA1I 265 CAGCTG C[AC]G^C[GT]G
674 CAGCTG
MvnI 27 CGCG CG^CG
165 CGCG
NciI 67 CCCGG CC^[GC]GG
68 CCGGG
PvuII 265 CAGCTG CAG^CTG
674 CAGCTG
Sau96I 65 GGCCC G^G[ACGT]CC
70 GGGCC
ScrFI 67 CCCGG CC^[ACGT]GG
68 CCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwI BbvI BsmAI
BstYI Cac8I DdeI DpnII Fnu4HI
HaeIII HapII HpaII ItaI MaeII
MboI MboII MflI MnlI MspI
MwoI NdeII NlaIII NlaIV Sau3AI
TseI Tsp509I XhoII