Restriction map for
pFN21AE5274
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BseRI BsgI BsiWI
BsiYI BslI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DdeI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MboII MfeI MflI
MluI MluNI MscI MslI MspA1I
MunI MvnI MwoI NaeI NarI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 461 TCCGGA T^CCGGA
AciI 426 GCGG CCGC(-3/-1)
AcsI 578 GAATTT [GA]^AATT[CT]
AlwI 450 GATCC GGATC(4/5)
AlwNI 41 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 578 GAATTT [GA]^AATT[CT]
AspHI 184 GAGCAC G[AT]GC[AT]^C
AviII 125 TGCGCA TGC^GCA
BbvI 123 GCTGC GCAGC(8/12)
BciVI 222 GGATAC GTATCC(6/5)
BclI 399 TGATCA T^GATCA
BcnI 276 CCGGG CC^[GC]GG
BmyI 184 GAGCAC G[AGT]GC[ACT]^C
BsaBI 444 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 461 TCCGGA [AT]^CCGG[AT]
BseAI 461 TCCGGA T^CCGGA
BsiEI 517 CGATCG CG[GA][CT]^CG
BsiHKAI 184 GAGCAC G[AT]GC[AT]^C
BsmAI 66 GTCTC GTCTC(1/5)
BsmI 457 GCATTC GAATGC(1/-1)
Bsp1286I 184 GAGCAC G[AGT]GC[ACT]^C
BspEI 461 TCCGGA T^CCGGA
BsrI 40 CCAGT ACTGG(1/-1)
CfoI 126 GCGC GCG^C
DraI 510 TTTAAA TTT^AAA
EaeI 436 CGGCCA [CT]^GGCC[GA]
FbaI 399 TGATCA T^GATCA
Fnu4HI 123 GCTGC GC^[ACGT]GC
FspI 125 TGCGCA TGC^GCA
HaeIII 437 GGCC GG^CC
HgaI 146 GCGTC GACGC(5/10)
HhaI 126 GCGC GCG^C
HinP1I 126 GCGC G^CGC
HphI 409 TCACC GGTGA(8/7)
ItaI 123 GCTGC GC^[ACGT]GC
MamI 444 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 461 TCCGGA T^CCGGA
NciI 276 CCGGG CC^[GC]GG
NcoI 106 CCATGG C^CATGG
NspI 419 GCATGT [GA]CATG^[CT]
PshAI 49 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 517 CGATCG CGAT^CG
SfaNI 128 GCATC GCATC(5/9)
SfcI 370 CTACAG C^T[GA][CT]AG
SspI 505 AATATT AAT^ATT
TaqI 182 TCGA T^CGA
TfiI 429 GATTC G^A[AT]TC
TseI 123 GCTGC G^C[AT]GC
TthHB8I 182 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 55 GGTCC G^G[AT]CC
491 GGACC
BstF5I 87 CATCC GGATG(2/0)
273 CATCC
BstNI 23 CCAGG CC^[AT]GG
640 CCAGG
Cfr13I 55 GGTCC G^G[ACGT]CC
491 GGACC
DsaI 106 CCATGG C^C[GA][CT]GG
433 CCACGG
EcoRII 23 CCAGG ^CC[AT]GG
640 CCAGG
FokI 87 CATCC GGATG(9/13)
273 CATCC
HapII 276 CCGG C^CGG
462 CCGG
HpaII 276 CCGG C^CGG
462 CCGG
MnlI 90 CCTC CCTC(7/6)
193 CCTC
MseI 98 TTAA T^TAA
511 TTAA
MspI 276 CCGG C^CGG
462 CCGG
MvaI 23 CCAGG CC^[AT]GG
640 CCAGG
NlaIII 107 CATG CATG^
420 CATG
PleI 644 GACTC GAGTC(4/5)
64 GAGTC
Sau96I 55 GGTCC G^G[ACGT]CC
491 GGACC
Tru9I 98 TTAA T^TAA
511 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BsaJI BssKI DpnII EcoT14I HinfI
MaeIII MboI NdeII Sau3AI ScrFI
StyI Tsp45I Tsp509I TspRI