(Linear) (Six-base) MAPSORT of: KIEE5274.seq  Check: 1347  from: 1  to: 654



KIEE5274.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     654 
   Size:        184     470
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       3      35     654 
   Size:          3      32     619

  Fragments arranged by size: 

                 619      32       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46     654 
   Size:         46     608
 
ApoI r'AATT_y

Cuts at:      0     578     654 
   Size:        578      76
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     216     654 
   Size:        216     438
 
BclI T'GATC_A

Cuts at:      0     399     654 
   Size:        399     255
 
BsaBI GATnn'nnATC

Cuts at:      0     448     654 
   Size:        448     206
 
BsaWI w'CCGG_w

Cuts at:      0     461     654 
   Size:        461     193
 
BseYI C'CCAG_C

Cuts at:      0      35     654 
   Size:         35     619
 
BsiEI CG_ry'CG

Cuts at:      0     520     654 
   Size:        520     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     654 
   Size:        188     466
 
BsmI GAATG_Cn'

Cuts at:      0     457     654 
   Size:        457     197
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     654 
   Size:        188     466
 
BspEI T'CCGG_A

Cuts at:      0     461     654 
   Size:        461     193
 
BtgI C'CryG_G

Cuts at:      0     106     433     654 
   Size:        106     327     221

  Fragments arranged by size: 

                 327     221     106
 
DraI TTT'AAA

Cuts at:      0     512     654 
   Size:        512     142
 
EaeI y'GGCC_r

Cuts at:      0     436     654 
   Size:        436     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     654 
   Size:        184     470
 
FspI TGC'GCA

Cuts at:      0     127     654 
   Size:        127     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     326     358     391     423     635     654 
   Size:         55      32     239      32      33      32     212      19

  Fragments arranged by size: 

                 239     212      55      33      32      32      32      19
            
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     654 
   Size:         53     601
 
NcoI C'CATG_G

Cuts at:      0     106     654 
   Size:        106     548
 
NspI r_CATG'y

Cuts at:      0     423     654 
   Size:        423     231
 
PshAI GACnn'nnGTC

Cuts at:      0      53     654 
   Size:         53     601
 
PvuI CG_AT'CG

Cuts at:      0     520     654 
   Size:        520     134
 
SfcI C'TryA_G

Cuts at:      0     370     654 
   Size:        370     284
 
SspI AAT'ATT

Cuts at:      0     507     654 
   Size:        507     147
 
StyI C'CwwG_G

Cuts at:      0     106     412     487     654 
   Size:        106     306      75     167

  Fragments arranged by size: 

                 306     167     106      75
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI    BciVI     BclI    BsaBI    BsaWI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI     BtgI     DraI
     EaeI  Eco57MI     FspI    Hin4I     MmeI     NcoI     NspI    PshAI
     PvuI     SfcI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI