(Circular) (Six-base) MAPSORT of: pFN21AE6482.seq Check: 6725 from: 1 to: 6319 pFN21AE6482 6319 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3119 3119 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3303 4129 278 Size: 53 83 186 936 1767 826 2468 Fragments arranged by size: 2468 1767 936 826 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 2630 3073 4382 5766 6034 1632 Size: 866 132 443 1309 1384 268 1917 Fragments arranged by size: 1917 1384 1309 866 443 268 132 AfeI AGC'GCT Cuts at: 2799 2799 Size: 6319 AflII C'TTAA_G Cuts at: 820 1017 3398 820 Size: 197 2381 3741 Fragments arranged by size: 3741 2381 197 AflIII A'CryG_T Cuts at: 1203 2595 5218 6195 1203 Size: 1392 2623 977 1327 Fragments arranged by size: 2623 1392 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 3237 3340 3574 4062 5634 3237 Size: 103 234 488 1572 3922 Fragments arranged by size: 3922 1572 488 234 103 ApaI G_GGCC'C Cuts at: 2199 2199 Size: 6319 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2533 3628 3854 4006 6115 1493 Size: 1040 1095 226 152 2109 1697 Fragments arranged by size: 2109 1697 1095 1040 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2307 2856 2904 3360 3593 3644 1870 Size: 73 364 549 48 456 233 51 4545 Fragments arranged by size: 4545 549 456 364 233 73 51 48 AvrII C'CTAG_G Cuts at: 2405 2405 Size: 6319 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2455 2488 2455 Size: 33 6286 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2828 2884 3365 3640 Size: 325 217 627 350 691 56 481 275 Cuts at: 3640 5095 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 691 627 481 350 325 275 217 56 BanII G_rGCy'C Cuts at: 1391 2021 2199 2208 2819 3568 3638 1391 Size: 630 178 9 611 749 70 4072 Fragments arranged by size: 4072 749 630 611 178 70 9 BbeI G_GCGC'C Cuts at: 3369 3369 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2736 2998 928 Size: 971 837 262 4249 Fragments arranged by size: 4249 971 837 262 BbvCI CC'TCA_GC Cuts at: 2505 2505 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2629 2663 4510 4544 1903 Size: 34 692 34 1847 34 3678 Fragments arranged by size: 3678 1847 692 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2203 2421 Size: 107 122 71 631 605 406 125 218 Cuts at: 2421 4930 136 Size: 2509 1525 Fragments arranged by size: 2509 1525 631 605 406 218 125 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3494 3567 3736 3494 Size: 73 169 6077 Fragments arranged by size: 6077 169 73 Bme1580I G_kGCm'C Cuts at: 1790 2142 2199 2642 3514 4380 5536 1790 Size: 352 57 443 872 866 1156 2573 Fragments arranged by size: 2573 1156 872 866 443 352 57 BmgBI CAC'GTC Cuts at: 1318 3430 1318 Size: 2112 4207 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2193 3225 4050 Size: 416 53 83 399 24 776 1032 825 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 1032 958 825 776 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2040 2315 3079 3619 2040 Size: 275 764 540 4740 Fragments arranged by size: 4740 764 540 275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 2680 3074 4979 1239 Size: 873 568 394 1905 2579 Fragments arranged by size: 2579 1905 873 568 394 Bpu10I CC'TnA_GC Cuts at: 2505 3047 2505 Size: 542 5777 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2341 3435 4982 882 Size: 617 842 1094 1547 2219 Fragments arranged by size: 2219 1547 1094 842 617 BsaAI yAC'GTr Cuts at: 493 1204 3316 4110 493 Size: 711 2112 794 2702 Fragments arranged by size: 2702 2112 794 711 BsaBI GATnn'nnATC Cuts at: 2783 6313 2783 Size: 3530 2789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2099 3300 3366 4126 Size: 53 83 186 936 566 1201 66 760 Cuts at: 4126 4508 5143 275 Size: 382 635 1451 Fragments arranged by size: 1451 1201 936 760 635 566 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 3303 4752 5150 5424 5571 5982 3303 Size: 1449 398 274 147 411 3640 Fragments arranged by size: 3640 1449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3434 3464 1178 Size: 30 165 30 2031 30 4033 Fragments arranged by size: 4033 2031 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2416 3176 3237 1186 Size: 880 350 760 61 4268 Fragments arranged by size: 4268 880 760 350 61 BseYI C'CCAG_C Cuts at: 2260 2585 2618 2969 3118 3237 3333 3421 5522 Size: 325 33 351 149 119 96 88 2101 Cuts at: 5522 6018 2260 Size: 496 2561 Fragments arranged by size: 2561 2101 496 351 325 149 119 96 88 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2266 2972 1163 Size: 573 530 706 4510 Fragments arranged by size: 4510 706 573 530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2646 3769 4530 5558 1540 Size: 275 186 645 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 645 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2361 2819 2964 4380 4465 5536 2021 Size: 340 458 145 1416 85 1071 2804 Fragments arranged by size: 2804 1416 1071 458 340 145 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2142 2199 2208 2361 2642 2819 Size: 399 231 121 57 9 153 281 177 Cuts at: 2819 2964 3514 3568 3638 4380 4465 5536 1391 Size: 145 550 54 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 399 281 231 177 153 145 121 85 70 57 54 9 BspEI T'CCGG_A Cuts at: 3303 5982 3303 Size: 2679 3640 BspHI T'CATG_A Cuts at: 3141 4208 5938 3141 Size: 1067 1730 3522 Fragments arranged by size: 3522 1730 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 3119 3674 844 Size: 1403 872 555 3489 Fragments arranged by size: 3489 1403 872 555 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2294 2456 2887 3616 4963 5150 Size: 110 227 257 162 431 729 1347 187 Cuts at: 5150 1700 Size: 2869 Fragments arranged by size: 2869 1347 729 431 257 227 187 162 110 BsrGI T'GTAC_A Cuts at: 96 2570 3469 96 Size: 2474 899 2946 Fragments arranged by size: 2946 2474 899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 2959 3577 3613 794 Size: 2165 618 36 3500 Fragments arranged by size: 3500 2165 618 36 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2125 Size: 6319 BstYI r'GATC_y Cuts at: 1972 3532 3649 4016 4401 4418 5870 5956 5968 Size: 1560 117 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1560 1452 385 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2517 3030 513 Size: 552 939 513 513 3802 Fragments arranged by size: 3802 939 552 513 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 3835 4629 4649 3835 Size: 794 20 5505 Fragments arranged by size: 5505 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2757 2792 529 Size: 35 2193 35 4056 Fragments arranged by size: 4056 2193 35 35 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 3274 3274 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2446 2889 Size: 54 1425 50 275 18 177 439 443 Cuts at: 2889 2988 3387 3766 4655 6284 8 Size: 99 399 379 889 1629 43 Fragments arranged by size: 1629 1425 889 443 439 399 379 275 177 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4249 1353 Size: 2896 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3068 4906 5280 5426 387 Size: 1064 138 1479 1838 374 146 1280 Fragments arranged by size: 1838 1479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2019 2817 2019 Size: 798 5521 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2498 2630 2680 3073 3074 4382 Size: 393 480 386 132 50 393 1 1308 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1308 787 597 480 393 393 386 268 132 50 1 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2219 2723 2048 Size: 171 504 5644 Fragments arranged by size: 5644 504 171 EcoO109I rG'GnC_Cy Cuts at: 2087 2195 2212 2614 2715 2944 3227 2087 Size: 108 17 402 101 229 283 5179 Fragments arranged by size: 5179 402 283 229 108 101 17 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2503 2503 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2496 2801 3369 5466 1678 Size: 818 305 568 2097 2531 Fragments arranged by size: 2531 2097 818 568 305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3434 3466 4960 4992 5034 Size: 32 135 32 1862 32 1494 32 42 Cuts at: 5034 5066 1373 Size: 32 2626 Fragments arranged by size: 2626 1862 1494 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 3365 3365 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2448 3389 10 Size: 54 1425 343 177 439 941 2940 Fragments arranged by size: 2940 1425 941 439 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4277 4636 4795 518 Size: 648 3111 359 159 2042 Fragments arranged by size: 3111 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2927 2956 2993 3337 3735 4412 5560 5805 Size: 1321 29 37 344 398 677 1148 245 Cuts at: 5805 1606 Size: 2120 Fragments arranged by size: 2120 1321 1148 677 398 344 245 37 29 NaeI GCC'GGC Cuts at: 2296 2296 Size: 6319 NarI GG'CG_CC Cuts at: 3366 3366 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2294 2294 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2599 3677 5222 1132 Size: 97 1370 1078 1545 2229 Fragments arranged by size: 2229 1545 1370 1078 97 PasI CC'CwG_GG Cuts at: 1348 2221 2287 2919 3436 1348 Size: 873 66 632 517 4231 Fragments arranged by size: 4231 873 632 517 66 PciI A'CATG_T Cuts at: 2595 5218 2595 Size: 2623 3696 PflMI CCAn_nnn'nTGG Cuts at: 1108 2851 3237 1108 Size: 1743 386 4190 Fragments arranged by size: 4190 1743 386 PfoI T'CCnGG_A Cuts at: 2089 2089 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 3316 3316 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 2212 2614 2715 2087 Size: 125 402 101 5691 Fragments arranged by size: 5691 402 125 101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2195 2195 Size: 6319 PspXI vC'TCGA_Gb Cuts at: 3593 3593 Size: 6319 PstI C_TGCA'G Cuts at: 830 2727 3671 830 Size: 1897 944 3478 Fragments arranged by size: 3478 1897 944 PvuI CG_AT'CG Cuts at: 2001 2646 2001 Size: 645 5674 PvuII CAG'CTG Cuts at: 2927 2956 3337 2927 Size: 29 381 5909 Fragments arranged by size: 5909 381 29 SacI G_AGCT'C Cuts at: 2021 2819 2021 Size: 798 5521 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 3012 3270 3012 Size: 258 6061 SfcI C'TryA_G Cuts at: 826 1045 2723 3667 4802 5483 5674 826 Size: 219 1678 944 1135 681 191 1471 Fragments arranged by size: 1678 1471 1135 944 681 219 191 SfoI GGC'GCC Cuts at: 3367 3367 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2309 2858 3362 3646 1872 Size: 437 549 504 284 4545 Fragments arranged by size: 4545 549 504 437 284 SmlI C'TyrA_G Cuts at: 820 1017 1943 3398 3593 4423 5324 5586 820 Size: 197 926 1455 195 830 901 262 1553 Fragments arranged by size: 1553 1455 926 901 830 262 197 195 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SrfI GCCC'GGGC Cuts at: 2309 2309 Size: 6319 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 2410 3257 1517 Size: 893 847 4579 Fragments arranged by size: 4579 893 847 StyI C'CwwG_G Cuts at: 513 1065 2004 2405 2892 3758 513 Size: 552 939 401 487 866 3074 Fragments arranged by size: 3074 939 866 552 487 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 2261 4346 4363 4516 4701 5146 Size: 1255 537 144 2085 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 2085 1498 1255 537 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2570 2929 3469 Size: 274 80 33 51 1443 593 359 540 Cuts at: 3469 4565 6277 96 Size: 1096 1712 138 Fragments arranged by size: 1712 1443 1096 593 540 359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 3593 1943 Size: 1650 4669 XmaI C'CCGG_G Cuts at: 1870 2307 2856 3360 3644 1870 Size: 437 549 504 284 4545 Fragments arranged by size: 4545 549 504 437 284 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3301 4127 276 Size: 53 83 186 936 1767 826 2468 Fragments arranged by size: 2468 1767 936 826 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SanDI SapI SfiI SwaI XcmI