Restriction map for
pFN21AE5166
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BclI BfrI BglI BglII BlnI
BmyI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 85 GTCTAC GT^[AC][GT]AC
AfaI 270 GTAC GT^AC
Asp700I 223 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 114 GGACC G^G[AT]CC
BcgI 349 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 8 GTATCC GTATCC(6/5)
BcnI 54 CCGGG CC^[GC]GG
BfaI 61 CTAG C^TAG
BlpI 409 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 409 GCTGAGC GC^T[ACGT]AGC
BsaI 456 GGTCTC GGTCTC(1/5)
BseRI 354 CTCCTC GAGGAG(10/8)
BsmAI 457 GTCTC GTCTC(1/5)
BsmFI 398 GTCCC GGGAC(10/14)
BspMI 300 GCAGGT ACCTGC(4/8)
BstEII 315 GGTTACC G^GT[ACGT]ACC
BstPI 315 GGTTACC G^GT[ACGT]ACC
CelII 409 GCTGAGC GC^T[ACGT]AGC
Cfr13I 114 GGACC G^G[ACGT]CC
EaeI 153 TGGCCG [CT]^GGCC[GA]
EcoO65I 315 GGTTACC G^GT[ACGT]ACC
EcoRV 175 GATATC GAT^ATC
HaeIII 154 GGCC GG^CC
HapII 54 CCGG C^CGG
HinfI 48 GAATC G^A[ACGT]TC
HpaII 54 CCGG C^CGG
MaeI 61 CTAG C^TAG
MspI 54 CCGG C^CGG
NciI 54 CCGGG CC^[GC]GG
NlaIII 202 CATG CATG^
PflMI 126 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 270 GTAC GT^AC
Sau96I 114 GGACC G^G[ACGT]CC
SspI 184 AATATT AAT^ATT
TaqI 242 TCGA T^CGA
TfiI 48 GAATC G^A[AT]TC
Tsp45I 158 GTGAC ^GT[GC]AC
TspRI 90 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 242 TCGA T^CGA
Van91I 126 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 223 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 127 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
376 CAGCAGCTG
BbsI 64 GAAGAC GAAGAC(2/6)
330 GAAGAC
BpmI 459 CTCCAG CTGGAG(16/14)
37 CTGGAG
BpuAI 64 GAAGAC GAAGAC(2/6)
330 GAAGAC
BsaJI 102 CCAGGG C^C[ACGT][ACGT]GG
356 CCTCGG
BsiYI 126 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCGAAAGAAGG
BslI 126 CCAGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
165 CCGAAAGAAGG
BsrI 234 ACTGG ACTGG(1/-1)
267 CCAGT
BstNI 36 CCTGG CC^[AT]GG
102 CCAGG
Cac8I 107 GCAAGC GC[ACGT]^[ACGT]GC
381 GCTGGC
EcoRII 36 CCTGG ^CC[AT]GG
102 CCAGG
MaeIII 158 GTGAC ^GT[ACGT]AC
316 GTTAC
MspA1I 127 CAGCTG C[AC]G^C[GT]G
379 CAGCTG
MvaI 36 CCTGG CC^[AT]GG
102 CCAGG
PvuII 127 CAGCTG CAG^CTG
379 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AluI BbvI BssKI BstF5I
DdeI Fnu4HI FokI ItaI MboII
MnlI MwoI ScrFI SfaNI TseI