(Circular) (Six-base) MAPSORT of: pFN21AE6477.seq Check: 9544 from: 1 to: 6220 pFN21AE6477 6220 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3417 4030 278 Size: 53 83 186 936 1881 613 2468 Fragments arranged by size: 2468 1881 936 613 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 3447 3563 1937 Size: 1077 433 116 4594 Fragments arranged by size: 4594 1077 433 116 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3290 4283 5667 5935 1632 Size: 1658 993 1384 268 1917 Fragments arranged by size: 1917 1658 1384 993 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2115 5119 6096 1203 Size: 912 3004 977 1327 Fragments arranged by size: 3004 1327 977 912 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2570 2736 2988 1166 Size: 1404 166 252 4398 Fragments arranged by size: 4398 1404 252 166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2048 2080 2048 Size: 32 6188 AlwNI CAG_nnn'CTG Cuts at: 2787 3963 5535 2787 Size: 1176 1572 3472 Fragments arranged by size: 3472 1572 1176 ApaI G_GGCC'C Cuts at: 2233 2233 Size: 6220 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2557 3529 3755 3907 6016 1493 Size: 1064 972 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 972 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BamHI G'GATC_C Cuts at: 2883 3550 3917 2883 Size: 667 367 5186 Fragments arranged by size: 5186 667 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3317 3541 4996 618 Size: 325 217 627 1530 224 1455 1842 Fragments arranged by size: 1842 1530 1455 627 325 224 217 BanII G_rGCy'C Cuts at: 1391 2233 2433 2591 3063 3363 3539 1391 Size: 842 200 158 472 300 176 4072 Fragments arranged by size: 4072 842 472 300 200 176 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3382 928 Size: 971 1483 3766 Fragments arranged by size: 3766 1483 971 BbvCI CC'TCA_GC Cuts at: 2727 2727 Size: 6220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2500 2534 2834 2868 4411 Size: 34 50 34 479 34 300 34 1543 Cuts at: 4411 4445 1903 Size: 34 3678 Fragments arranged by size: 3678 1543 479 300 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 3186 1548 Size: 1638 4582 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2978 2999 3273 Size: 107 122 71 631 605 1306 21 274 Cuts at: 3273 4831 136 Size: 1558 1525 Fragments arranged by size: 1558 1525 1306 631 605 274 122 107 71 21 BglII A'GATC_T Cuts at: 3456 6215 3456 Size: 2759 3461 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2233 4281 5437 1790 Size: 443 2048 1156 2573 Fragments arranged by size: 2573 2048 1156 443 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 4880 1239 Size: 796 2845 2579 Fragments arranged by size: 2845 2579 796 Bpu10I CC'TnA_GC Cuts at: 2727 3479 2727 Size: 752 5468 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 4345 5210 5508 2831 Size: 1514 865 298 3543 Fragments arranged by size: 3543 1514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 3185 6214 3185 Size: 3029 3191 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2670 3414 4027 4409 Size: 53 83 186 936 1137 744 613 382 Cuts at: 4409 5044 275 Size: 635 1451 Fragments arranged by size: 1451 1137 936 744 635 613 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 4653 5051 5325 5472 5883 2880 Size: 1773 398 274 147 411 3217 Fragments arranged by size: 3217 1773 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2715 1186 Size: 1529 4691 BseYI C'CCAG_C Cuts at: 2226 3002 3356 5423 5919 2226 Size: 776 354 2067 496 2527 Fragments arranged by size: 2527 2067 776 496 354 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2242 1163 Size: 573 402 104 5141 Fragments arranged by size: 5141 573 402 104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2433 4281 4366 5437 2433 Size: 1848 85 1071 3216 Fragments arranged by size: 3216 1848 1071 85 BsmI GAATG_Cn' Cuts at: 2373 3731 3824 2373 Size: 1358 93 4769 Fragments arranged by size: 4769 1358 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3070 3070 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2433 2591 3063 3363 3539 4281 Size: 399 443 200 158 472 300 176 742 Cuts at: 4281 4366 5437 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 443 399 300 200 176 158 85 BspEI T'CCGG_A Cuts at: 2880 5883 2880 Size: 3003 3217 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2335 2344 2805 4107 1394 Size: 429 512 9 461 1302 3507 Fragments arranged by size: 3507 1302 512 461 429 9 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2600 3320 3438 4864 5051 1700 Size: 110 790 720 118 1426 187 2869 Fragments arranged by size: 2869 1426 790 720 187 118 110 BsrGI T'GTAC_A Cuts at: 96 3138 96 Size: 3042 3178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 2426 4280 5292 2426 Size: 1854 1012 3354 Fragments arranged by size: 3354 1854 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 2497 1184 Size: 1313 4907 BstYI r'GATC_y Cuts at: 1972 2883 3456 3550 3917 4302 4319 5771 5857 Size: 911 573 94 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 911 573 385 367 346 94 86 17 12 BstZ17I GTA'TAC Cuts at: 3448 3448 Size: 6220 Bsu36I CC'TnA_GG Cuts at: 2766 2766 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2734 3294 3372 513 Size: 552 1669 560 78 3361 Fragments arranged by size: 3361 1669 560 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2275 2953 487 Size: 683 56 1049 678 3754 Fragments arranged by size: 3754 1049 683 678 56 BtsI GCAGTG_nn' Cuts at: 2627 3231 3736 4530 4550 2627 Size: 604 505 794 20 4297 Fragments arranged by size: 4297 794 604 505 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 2217 2217 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2820 3151 3303 Size: 54 1425 50 275 18 990 331 152 Cuts at: 3303 3667 4556 6185 8 Size: 364 889 1629 43 Fragments arranged by size: 1629 1425 990 889 364 331 275 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2690 2841 3026 4150 1353 Size: 1337 151 185 1124 3423 Fragments arranged by size: 3423 1337 1124 185 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2431 Size: 6220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 3290 4283 4880 5667 5935 1239 Size: 393 403 1255 993 597 787 268 1524 Fragments arranged by size: 1524 1255 993 787 597 403 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2050 2050 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2230 2626 2770 3007 3199 3344 2230 Size: 396 144 237 192 145 5106 Fragments arranged by size: 5106 396 237 192 145 144 EcoRI G'AATT_C Cuts at: 2557 3529 3907 2557 Size: 972 378 4870 Fragments arranged by size: 4870 972 378 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2879 5367 1678 Size: 1201 2488 2531 Fragments arranged by size: 2531 2488 1201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 4861 4893 4935 Size: 32 135 32 489 32 2768 32 42 Cuts at: 4935 4967 1373 Size: 32 2626 Fragments arranged by size: 2768 2626 489 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 3153 10 Size: 54 1425 343 990 331 3077 Fragments arranged by size: 3077 1425 990 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2570 2609 2736 2988 3179 4178 Size: 648 1140 264 39 127 252 191 999 Cuts at: 4178 4537 4696 518 Size: 359 159 2042 Fragments arranged by size: 2042 1140 999 648 359 264 252 191 159 127 39 MspA1I CmG'CkG Cuts at: 1606 2061 2226 2363 2747 3492 3636 4313 5461 Size: 455 165 137 384 745 144 677 1148 Cuts at: 5461 5706 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 745 677 455 384 245 165 144 137 NaeI GCC'GGC Cuts at: 2602 2602 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2600 2600 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2119 2311 3400 3578 5123 1132 Size: 97 890 192 1089 178 1545 2229 Fragments arranged by size: 2229 1545 1089 890 192 178 97 PasI CC'CwG_GG Cuts at: 1348 2774 1348 Size: 1426 4794 PciI A'CATG_T Cuts at: 2115 5119 2115 Size: 3004 3216 PflMI CCAn_nnn'nTGG Cuts at: 1108 2484 3378 1108 Size: 1376 894 3950 Fragments arranged by size: 3950 1376 894 PfoI T'CCnGG_A Cuts at: 2023 2120 2023 Size: 97 6123 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2770 3007 2770 Size: 237 5983 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2229 2229 Size: 6220 PstI C_TGCA'G Cuts at: 830 3572 830 Size: 2742 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2061 2226 2363 2747 2061 Size: 165 137 384 5534 Fragments arranged by size: 5534 384 165 137 SacI G_AGCT'C Cuts at: 2433 2433 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SapI GCTCTTCn'nnn_ Cuts at: 2841 2841 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 3516 3516 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 3568 4703 5384 5575 826 Size: 219 2523 1135 681 191 1471 Fragments arranged by size: 2523 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 4324 5225 5487 820 Size: 197 926 867 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 2073 1517 Size: 556 5664 StyI C'CwwG_G Cuts at: 513 1065 2832 2994 3659 513 Size: 552 1767 162 665 3074 Fragments arranged by size: 3074 1767 665 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2599 2715 4247 4264 4417 4602 5047 Size: 1255 1019 116 1532 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1532 1498 1255 1019 445 185 153 116 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3138 4466 6178 Size: 274 80 33 51 1443 1161 1328 1712 Cuts at: 6178 96 Size: 138 Fragments arranged by size: 1712 1443 1328 1161 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2459 2459 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3415 4028 276 Size: 53 83 186 936 1881 613 2468 Fragments arranged by size: 2468 1881 936 613 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI