(Linear) (Six-base) MAPSORT of: KIEE6477.seq Check: 2701 from: 1 to: 1515 KIEE6477.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AatII G_ACGT'C Cuts at: 0 1412 1515 Size: 1412 103 AccI GT'mk_AC Cuts at: 0 1009 1442 1515 Size: 1009 433 73 Fragments arranged by size: 1009 433 73 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1285 1515 Size: 1285 230 AflIII A'CryG_T Cuts at: 0 110 1515 Size: 110 1405 AleI CACnn'nnGTG Cuts at: 0 565 731 983 1515 Size: 565 166 252 532 Fragments arranged by size: 565 532 252 166 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 43 75 1515 Size: 43 32 1440 Fragments arranged by size: 1440 43 32 AlwNI CAG_nnn'CTG Cuts at: 0 782 1515 Size: 782 733 ApaI G_GGCC'C Cuts at: 0 228 1515 Size: 228 1287 ApoI r'AATT_y Cuts at: 0 552 1515 Size: 552 963 BamHI G'GATC_C Cuts at: 0 878 1515 Size: 878 637 BanI G'GyrC_C Cuts at: 0 1312 1515 Size: 1312 203 BanII G_rGCy'C Cuts at: 0 228 428 586 1058 1358 1515 Size: 228 200 158 472 300 157 Fragments arranged by size: 472 300 228 200 158 157 BbsI GAAGACnn'nnnn_ Cuts at: 0 1377 1515 Size: 1377 138 BbvCI CC'TCA_GC Cuts at: 0 722 1515 Size: 722 793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 495 529 829 863 1515 Size: 495 34 300 34 652 Fragments arranged by size: 652 495 300 34 34 BclI T'GATC_A Cuts at: 0 1181 1515 Size: 1181 334 BglI GCCn_nnn'nGGC Cuts at: 0 973 994 1268 1515 Size: 973 21 274 247 Fragments arranged by size: 973 274 247 21 BglII A'GATC_T Cuts at: 0 1451 1515 Size: 1451 64 Bme1580I G_kGCm'C Cuts at: 0 228 1515 Size: 228 1287 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 1515 Size: 30 1485 Bpu10I CC'TnA_GC Cuts at: 0 722 1474 1515 Size: 722 752 41 Fragments arranged by size: 752 722 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 826 1515 Size: 826 689 BsaBI GATnn'nnATC Cuts at: 0 1180 1515 Size: 1180 335 BsaHI Gr'CG_yC Cuts at: 0 665 1409 1515 Size: 665 744 106 Fragments arranged by size: 744 665 106 BsaWI w'CCGG_w Cuts at: 0 875 1515 Size: 875 640 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 710 1515 Size: 710 805 BseYI C'CCAG_C Cuts at: 0 221 997 1351 1515 Size: 221 776 354 164 Fragments arranged by size: 776 354 221 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 237 1515 Size: 133 104 1278 Fragments arranged by size: 1278 133 104 BsiHKAI G_wGCw'C Cuts at: 0 428 1515 Size: 428 1087 BsmI GAATG_Cn' Cuts at: 0 368 1515 Size: 368 1147 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1065 1515 Size: 1065 450 Bsp1286I G_dGCh'C Cuts at: 0 228 428 586 1058 1358 1515 Size: 228 200 158 472 300 157 Fragments arranged by size: 472 300 228 200 158 157 BspEI T'CCGG_A Cuts at: 0 875 1515 Size: 875 640 BsrBI CCG'CTC Cuts at: 0 330 339 800 1515 Size: 330 9 461 715 Fragments arranged by size: 715 461 330 9 BsrFI r'CCGG_y Cuts at: 0 595 1315 1433 1515 Size: 595 720 118 82 Fragments arranged by size: 720 595 118 82 BsrGI T'GTAC_A Cuts at: 0 1133 1515 Size: 1133 382 BssSI C'ACGA_G Cuts at: 0 421 1515 Size: 421 1094 BstEII G'GTnAC_C Cuts at: 0 492 1515 Size: 492 1023 BstYI r'GATC_y Cuts at: 0 878 1451 1515 Size: 878 573 64 Fragments arranged by size: 878 573 64 BstZ17I GTA'TAC Cuts at: 0 1443 1515 Size: 1443 72 Bsu36I CC'TnA_GG Cuts at: 0 761 1515 Size: 761 754 BtgI C'CryG_G Cuts at: 0 729 1289 1367 1515 Size: 729 560 78 148 Fragments arranged by size: 729 560 148 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 270 948 1515 Size: 270 678 567 Fragments arranged by size: 678 567 270 BtsI GCAGTG_nn' Cuts at: 0 622 1226 1515 Size: 622 604 289 Fragments arranged by size: 622 604 289 DraIII CAC_nnn'GTG Cuts at: 0 212 1515 Size: 212 1303 EaeI y'GGCC_r Cuts at: 0 815 1146 1298 1515 Size: 815 331 152 217 Fragments arranged by size: 815 331 217 152 EarI CTCTTCn'nnn_ Cuts at: 0 685 836 1021 1515 Size: 685 151 185 494 Fragments arranged by size: 685 494 185 151 EcoICRI GAG'CTC Cuts at: 0 426 1515 Size: 426 1089 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 1285 1515 Size: 30 1255 230 Fragments arranged by size: 1255 230 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 45 1515 Size: 45 1470 EcoO109I rG'GnC_Cy Cuts at: 0 225 621 765 1002 1194 1339 1515 Size: 225 396 144 237 192 145 176 Fragments arranged by size: 396 237 225 192 176 145 144 EcoRI G'AATT_C Cuts at: 0 552 1515 Size: 552 963 HaeII r_GCGC'y Cuts at: 0 874 1515 Size: 874 641 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 56 88 1515 Size: 56 32 1427 Fragments arranged by size: 1427 56 32 MscI TGG'CCA Cuts at: 0 817 1148 1515 Size: 817 331 367 Fragments arranged by size: 817 367 331 MslI CAynn'nnrTG Cuts at: 0 301 565 604 731 983 1174 1515 Size: 301 264 39 127 252 191 341 Fragments arranged by size: 341 301 264 252 191 127 39 MspA1I CmG'CkG Cuts at: 0 56 221 358 742 1487 1515 Size: 56 165 137 384 745 28 Fragments arranged by size: 745 384 165 137 56 28 NaeI GCC'GGC Cuts at: 0 597 1515 Size: 597 918 NgoMIV G'CCGG_C Cuts at: 0 595 1515 Size: 595 920 NspI r_CATG'y Cuts at: 0 114 306 1395 1515 Size: 114 192 1089 120 Fragments arranged by size: 1089 192 120 114 PasI CC'CwG_GG Cuts at: 0 769 1515 Size: 769 746 PciI A'CATG_T Cuts at: 0 110 1515 Size: 110 1405 PflMI CCAn_nnn'nTGG Cuts at: 0 479 1373 1515 Size: 479 894 142 Fragments arranged by size: 894 479 142 PfoI T'CCnGG_A Cuts at: 0 18 115 1515 Size: 18 97 1400 Fragments arranged by size: 1400 97 18 PpuMI rG'GwC_Cy Cuts at: 0 765 1002 1515 Size: 765 237 513 Fragments arranged by size: 765 513 237 PspOMI G'GGCC_C Cuts at: 0 224 1515 Size: 224 1291 PvuII CAG'CTG Cuts at: 0 56 221 358 742 1515 Size: 56 165 137 384 773 Fragments arranged by size: 773 384 165 137 56 SacI G_AGCT'C Cuts at: 0 428 1515 Size: 428 1087 SapI GCTCTTCn'nnn_ Cuts at: 0 836 1515 Size: 836 679 SmlI C'TyrA_G Cuts at: 0 805 1515 Size: 805 710 StuI AGG'CCT Cuts at: 0 68 1515 Size: 68 1447 StyI C'CwwG_G Cuts at: 0 827 989 1515 Size: 827 162 526 Fragments arranged by size: 827 526 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 594 710 1515 Size: 594 116 805 Fragments arranged by size: 805 594 116 TatI w'GTAC_w Cuts at: 0 1133 1515 Size: 1133 382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 454 1515 Size: 454 1061 ZraI GAC'GTC Cuts at: 0 1410 1515 Size: 1410 105 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI AlwNI ApaI ApoI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI BsrGI BssSI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI SapI SmlI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaXI BsiEI BsiWI BspHI BspMI BsrDI BssHII BstAPI BstBI BstXI ClaI CspCI DraI DrdI EagI EciI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI