Restriction map for
pFN21AE6675
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccIII AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RcaI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AatII 823 GACGTC GACGT^C
AccII 262 CGCG CG^CG
AclI 112 AACGTT AA^CGTT
AcsI 709 GAATTT [GA]^AATT[CT]
AcyI 823 GACGTC G[GA]^CG[CT]C
AflIII 508 ACATGT A^C[GA][CT]GT
Alw44I 331 GTGCAC G^TGCAC
ApaLI 331 GTGCAC G^TGCAC
ApoI 709 GAATTT [GA]^AATT[CT]
AspI 387 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 234 TGCGCA TGC^GCA
BamHI 73 GGATCC G^GATCC
BanI 580 GGCACC G^G[CT][GA]CC
BanII 51 GAGCTC G[GA]GC[CT]^C
BbvI 237 GCAGC GCAGC(8/12)
BciVI 723 GTATCC GTATCC(6/5)
BcnI 11 CCCGG CC^[GC]GG
BpmI 324 CTGGAG CTGGAG(16/14)
BsaBI 801 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 823 GACGTC G[GA]^CG[CT]C
BsaI 285 GAGACC GGTCTC(1/5)
BseRI 214 CTCCTC GAGGAG(10/8)
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsmAI 285 GAGAC GTCTC(1/5)
BsmI 225 GAATGC GAATGC(1/-1)
BspLU11I 508 ACATGT A^CATGT
BsrFI 16 ACCGGC [GA]^CCGG[CT]
BstUI 262 CGCG CG^CG
BstXI 389 CCATGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 73 GGATCC [GA]^GATC[CT]
Cfr10I 16 ACCGGC [GA]^CCGG[CT]
CpoI 13 CGGACCG CG^G[AT]CCG
EagI 18 CGGCCG C^GGCCG
EarI 217 CTCTTC CTCTTC(1/4)
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
EcoNI 320 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 420 GATATC GAT^ATC
FauI 299 CCCGC CCCGC(4/6)
FspI 234 TGCGCA TGC^GCA
HgaI 62 GCGTC GACGC(5/10)
Hin1I 823 GACGTC G[GA]^CG[CT]C
HinfI 442 GATTC G^A[ACGT]TC
Ksp632I 217 CTCTTC CTCTTC(1/4)
MaeIII 385 GTGAC ^GT[ACGT]AC
MamI 801 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 73 GGATCC [GA]^GATC[CT]
MseI 835 TTAA T^TAA
MvnI 262 CGCG CG^CG
NciI 11 CCCGG CC^[GC]GG
NspI 508 ACATGT [GA]CATG^[CT]
PflFI 387 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 112 AACGTT AA^CGTT
RsrII 13 CGGACCG CG^G[AT]CCG
SacI 51 GAGCTC GAGCT^C
ScaI 362 AGTACT AGT^ACT
SspI 187 AATATT AAT^ATT
StuI 24 AGGCCT AGG^CCT
TfiI 442 GATTC G^A[AT]TC
Tru9I 835 TTAA T^TAA
TseI 237 GCAGC G^C[AT]GC
Tsp45I 385 GTGAC ^GT[GC]AC
Tth111I 387 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 614 CCATACAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 73 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 306 GTAC GT^AC
363 GTAC
BbsI 820 GAAGAC GAAGAC(2/6)
909 GAAGAC
BpuAI 820 GAAGAC GAAGAC(2/6)
909 GAAGAC
BsmFI 180 GTCCC GGGAC(10/14)
763 GTCCC
BspMI 137 ACCTGC ACCTGC(4/8)
542 ACCTGC
DpnII 74 GATC ^GATC
754 GATC
DraII 178 AGGTCCC [GA]G^G[ACGT]CC[CT]
761 AGGTCCC
EaeI 18 CGGCCG [CT]^GGCC[GA]
662 TGGCCG
EcoO109I 178 AGGTCCC [GA]G^G[ACGT]CC[CT]
761 AGGTCCC
EcoT14I 704 CCAAGG C^C[AT][AT]GG
728 CCTTGG
HapII 12 CCGG C^CGG
17 CCGG
HpaII 12 CCGG C^CGG
17 CCGG
MboI 74 GATC ^GATC
754 GATC
MspI 12 CCGG C^CGG
17 CCGG
NdeII 74 GATC ^GATC
754 GATC
PpuMI 178 AGGTCCC [GA]G^G[AT]CC[CT]
761 AGGTCCC
RsaI 306 GTAC GT^AC
363 GTAC
Sau3AI 74 GATC ^GATC
754 GATC
SfaNI 103 GATGC GCATC(5/9)
202 GCATC
StyI 704 CCAAGG C^C[AT][AT]GG
728 CCTTGG
TaqI 94 TCGA T^CGA
513 TCGA
Tsp509I 710 AATT ^AATT
861 AATT
TthHB8I 94 TCGA T^CGA
513 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BmyI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MaeII
MboII MnlI MslI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TspRI