Restriction map for
pFN21AE6675
(Circular) (Six-base) MAPSORT of: pFN21AE6675.seq Check: 907 from: 1 to: 5641
pFN21AE6675 5641 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2832 3451 278
Size: 53 83 186 936 1296 619 2468
Fragments arranged by size:
2468 1296 936 619 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 2118 3768 4141 1563
Size: 555 1650 373 3063
Fragments arranged by size:
3063 1650 555 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2683 2933 3704 5088 5356 1632
Size: 1051 250 771 1384 268 1917
Fragments arranged by size:
1917 1384 1051 771 268 250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2513 4540 5517 1203
Size: 1310 2027 977 1327
Fragments arranged by size:
2027 1327 1310 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 2336 3698 4854 2336
Size: 1362 1156 3123
Fragments arranged by size:
3123 1362 1156
ApoI r'AATT_y
Cuts at: 1493 2714 2950 3176 3328 5437 1493
Size: 1221 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1221 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2260 2579 2762 2966 1870
Size: 73 317 319 183 204 4545
Fragments arranged by size:
4545 319 317 204 183 73
BamHI G'GATC_C
Cuts at: 2078 2971 3338 2078
Size: 893 367 4381
Fragments arranged by size:
4381 893 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2585 2962 4417 618
Size: 325 217 627 798 377 1455 1842
Fragments arranged by size:
1842 1455 798 627 377 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 2960 1391
Size: 669 900 4072
Fragments arranged by size:
4072 900 669
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 2921 928
Size: 971 933 89 3648
Fragments arranged by size:
3648 971 933 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3832 3866 1903
Size: 34 50 34 1811 34 3678
Fragments arranged by size:
3678 1811 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2739 3533 4743 2739
Size: 794 1210 3637
Fragments arranged by size:
3637 1210 794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2588 3702 4858 1790
Size: 550 248 1114 1156 2573
Fragments arranged by size:
2573 1156 1114 550 248
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 3372 4330
Size: 416 53 83 399 24 942 1013 958
Cuts at: 4330 442
Size: 1753
Fragments arranged by size:
1753 1013 958 942 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 2051
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 4301 1239
Size: 1111 1951 2579
Fragments arranged by size:
2579 1951 1111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2284 4304 882
Size: 617 785 2020 2219
Fragments arranged by size:
2219 2020 785 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 2810 5635 2810
Size: 2825 2816
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2829 3448 3830 4465
Size: 53 83 186 936 1296 619 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 1296 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 4483
Fragments arranged by size:
4483 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4617
BseYI C'CCAG_C
Cuts at: 2041 4844 5340 2041
Size: 2803 496 2342
Fragments arranged by size:
2803 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 3091 3852 4880 1540
Size: 275 186 25 1065 761 1028 2301
Fragments arranged by size:
2301 1065 1028 761 275 186 25
BsiHKAI G_wGCw'C
Cuts at: 2060 2340 2609 3702 3787 4858 2060
Size: 280 269 1093 85 1071 2843
Fragments arranged by size:
2843 1093 1071 280 269 85
BsmI GAATG_Cn'
Cuts at: 2236 3152 3245 2236
Size: 916 93 4632
Fragments arranged by size:
4632 916 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2340 2588 2609 2960 3702 3787
Size: 399 270 280 248 21 351 742 85
Cuts at: 3787 4858 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 280 270 248
85 21
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2556 2996 844
Size: 1307 405 440 3489
Fragments arranged by size:
3489 1307 440 405
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4136 4310 65
Size: 1183 2888 174 1396
Fragments arranged by size:
2888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 4285 4472 1700
Size: 110 211 2264 187 2869
Fragments arranged by size:
2869 2264 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2401
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2078 2971 3338 3723 3740 5192 5278 5290
Size: 106 893 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 893 385 367 346 106 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 3157 3951 3971 2256
Size: 901 794 20 3926
Fragments arranged by size:
3926 901 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2853 2888 529
Size: 35 2289 35 3282
Fragments arranged by size:
3282 2289 35 35
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2667 3088
Size: 54 1425 50 275 18 193 644 421
Cuts at: 3088 3977 5606 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 644 421 275 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2023 3088 1537
Size: 275 211 1065 4090
Fragments arranged by size:
4090 1065 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 3571 1353
Size: 875 1343 3423
Fragments arranged by size:
3423 1343 875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2058 2058
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2683 2933 3704 4301 5088 5356
Size: 393 718 333 250 771 597 787 268
Cuts at: 5356 1239
Size: 1524
Fragments arranged by size:
1524 787 771 718 597 393 333 268
250
EcoNI CCTnn'n_nnAGG
Cuts at: 2329 2329
Size: 5641
EcoO109I rG'GnC_Cy
Cuts at: 2184 2767 2184
Size: 583 5058
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
EcoRV GAT'ATC
Cuts at: 2427 2427
Size: 5641
FspI TGC'GCA
Cuts at: 2241 4147 2241
Size: 1906 3735
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 4282 4314 4356
Size: 32 135 32 734 32 1944 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1944 734 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 4755 4939 1305
Size: 1305 2145 184 2007
Fragments arranged by size:
2145 2007 1305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2399 2442 2849 3599 3958 4117
Size: 648 966 267 43 407 750 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 966 750 648 407 359 267 159
43
MspA1I CmG'CkG
Cuts at: 1606 3057 3734 4882 5127 1606
Size: 1451 677 1148 245 2120
Fragments arranged by size:
2120 1451 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2517 2999 4544 1132
Size: 97 1288 482 1545 2229
Fragments arranged by size:
2229 1545 1288 482 97
PasI CC'CwG_GG
Cuts at: 1348 2155 2420 1348
Size: 807 265 4569
Fragments arranged by size:
4569 807 265
PciI A'CATG_T
Cuts at: 2513 4540 2513
Size: 2027 3614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 2767 2184
Size: 583 5058
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 5641
SacI G_AGCT'C
Cuts at: 2060 2060
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 2369 3889 5601 2369
Size: 1520 1712 2409
Fragments arranged by size:
2409 1712 1520
SfcI C'TryA_G
Cuts at: 826 1045 2989 4124 4805 4996 826
Size: 219 1944 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 2194 3565 5
Size: 47 2142 1371 2081
Fragments arranged by size:
2142 2081 1371 47
StuI AGG'CCT
Cuts at: 1517 2031 1517
Size: 514 5127
StyI C'CwwG_G
Cuts at: 513 1065 2709 2733 3080 513
Size: 552 1644 24 347 3074
Fragments arranged by size:
3074 1644 552 347 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 3887 5599
Size: 274 80 33 51 1443 390 1520 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1520 1443 390 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 2395 1316
Size: 1079 4562
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2626
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2830 3449 276
Size: 53 83 186 936 1296 619 2468
Fragments arranged by size:
2468 1296 936 619 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI RsrII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI