################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 104 GGTACC G^GTACC AluI 218 AGCT AG^CT Asp700I 12 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 104 GGTACC G^GTACC BanII 274 GAGCCC G[GA]GC[CT]^C BbsI 96 GAAGAC GAAGAC(2/6) BcnI 268 CCGGG CC^[GC]GG BglI 174 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 57 AGATCT A^GATCT BmyI 274 GAGCCC G[AGT]GC[ACT]^C BpuAI 96 GAAGAC GAAGAC(2/6) BsmAI 162 GTCTC GTCTC(1/5) BsmFI 69 GTCCC GGGAC(10/14) Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C BspMI 90 GCAGGT ACCTGC(4/8) BsrFI 255 ACCGGC [GA]^CCGG[CT] BsrI 84 CCAGT ACTGG(1/-1) BssHII 234 GCGCGC G^CGCGC BstYI 57 AGATCT [GA]^GATC[CT] Cfr10I 255 ACCGGC [GA]^CCGG[CT] DpnII 58 GATC ^GATC HphI 93 GGTGA GGTGA(8/7) KpnI 104 GGTACC GGTAC^C MaeII 100 ACGT A^CGT MboI 58 GATC ^GATC MflI 57 AGATCT [GA]^GATC[CT] NciI 268 CCGGG CC^[GC]GG NdeII 58 GATC ^GATC PstI 142 CTGCAG CTGCA^G Sau3AI 58 GATC ^GATC SfaNI 244 GATGC GCATC(5/9) SfcI 142 CTGCAG C^T[GA][CT]AG TaqI 20 TCGA T^CGA TfiI 111 GAATC G^A[AT]TC Tsp509I 201 AATT ^AATT TthHB8I 20 TCGA T^CGA XhoII 57 AGATCT [GA]^GATC[CT] XmnI 12 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 235 CGCG CG^CG 292 CGCG AfaI 33 GTAC GT^AC 105 GTAC BanI 104 GGTACC G^G[CT][GA]CC 264 GGTGCC BbvI 216 GCAGC GCAGC(8/12) 141 GCTGC BpmI 7 CTGGAG CTGGAG(16/14) 157 CTGGAG BstF5I 119 GGATG GGATG(2/0) 243 GGATG BstUI 235 CGCG CG^CG 292 CGCG CfoI 234 GCGC GCG^C 236 GCGC EcoT14I 176 CCAAGG C^C[AT][AT]GG 239 CCAAGG FokI 119 GGATG GGATG(9/13) 243 GGATG HhaI 234 GCGC GCG^C 236 GCGC HinP1I 234 GCGC G^CGC 236 GCGC MvnI 235 CGCG CG^CG 292 CGCG PleI 67 GAGTC GAGTC(4/5) 160 GAGTC RsaI 33 GTAC GT^AC 105 GTAC StyI 176 CCAAGG C^C[AT][AT]GG 239 CCAAGG TseI 141 GCTGC G^C[AT]GC 216 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIV Sau96I ScrFI