Restriction map for
pFN21AE6583
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfaNI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 111 GTGCAC G^TGCAC
AlwI 27 GATCC GGATC(4/5)
ApaI 70 GGGCCC GGGCC^C
ApaLI 111 GTGCAC G^TGCAC
AvaII 99 GGACC G^G[AT]CC
BanI 63 GGTGCC G^G[CT][GA]CC
BanII 70 GGGCCC G[GA]GC[CT]^C
BfaI 187 CTAG C^TAG
BglI 72 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 129 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 129 GCTGAGC GC^T[ACGT]AGC
BsaI 259 GAGACC GGTCTC(1/5)
BsmAI 259 GAGAC GTCTC(1/5)
BsmFI 98 GGGAC GGGAC(10/14)
BstYI 26 AGATCC [GA]^GATC[CT]
CelII 129 GCTGAGC GC^T[ACGT]AGC
DdeI 130 CTGAG C^T[ACGT]AG
DpnII 27 GATC ^GATC
DsaI 59 CCATGG C^C[GA][CT]GG
HinfI 326 GAATC G^A[ACGT]TC
HphI 312 GGTGA GGTGA(8/7)
MaeI 187 CTAG C^TAG
MaeIII 218 GTGAC ^GT[ACGT]AC
MboI 27 GATC ^GATC
MflI 26 AGATCC [GA]^GATC[CT]
NcoI 59 CCATGG C^CATGG
NdeII 27 GATC ^GATC
PpuMI 98 GGGACCC [GA]G^G[AT]CC[CT]
PstI 180 CTGCAG CTGCA^G
Sau3AI 27 GATC ^GATC
SbfI 179 CCTGCAGG CCTGCA^GG
SfcI 180 CTGCAG C^T[GA][CT]AG
SfiI 71 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 276 CTCAAG C^T[CT][GA]AG
Sse8387I 179 CCTGCAGG CCTGCA^GG
StuI 224 AGGCCT AGG^CCT
TfiI 326 GAATC G^A[AT]TC
Tsp45I 218 GTGAC ^GT[GC]AC
XhoII 26 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 239 AGCT AG^CT
338 AGCT
AspHI 111 GTGCAC G[AT]GC[AT]^C
302 GTGCTC
BpmI 305 CTCCAG CTGGAG(16/14)
10 CTGGAG
BseRI 193 GAGGAG GAGGAG(10/8)
235 GAGGAG
BsiHKAI 111 GTGCAC G[AT]GC[AT]^C
302 GTGCTC
BsiYI 143 CCAGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
263 CCTGGGCCTGG
BslI 143 CCAGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
263 CCTGGGCCTGG
BsrI 40 ACTGG ACTGG(1/-1)
108 CCAGT
BstXI 222 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
323 CCAGAATCCTGG
DraII 69 AGGGCCC [GA]G^G[ACGT]CC[CT]
98 GGGACCC
EcoO109I 69 AGGGCCC [GA]G^G[ACGT]CC[CT]
98 GGGACCC
EcoT14I 59 CCATGG C^C[AT][AT]GG
169 CCAAGG
FauI 73 CCCGC CCCGC(4/6)
204 CCCGC
MwoI 72 GCCCGCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
198 GCCACCCCCGC
NlaIII 60 CATG CATG^
92 CATG
StyI 59 CCATGG C^C[AT][AT]GG
169 CCAAGG
XcmI 30 CCAGGCCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
85 CCAGGCTCATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BmyI BsaJI Bsp1286I
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI HaeIII ItaI MnlI MvaI
NlaIV Sau96I ScrFI TseI TspRI