(Linear) (Six-base) MAPSORT of: KIEE6583.seq  Check: 2559  from: 1  to: 351



KIEE6583.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67     351 
   Size:         67     284
 
ApaI G_GGCC'C

Cuts at:      0      74     351 
   Size:         74     277
 
ApaLI G'TGCA_C

Cuts at:      0     111     351 
   Size:        111     240
 
BanI G'GyrC_C

Cuts at:      0      63     351 
   Size:         63     288
 
BanII G_rGCy'C

Cuts at:      0      74     351 
   Size:         74     277
 
BglI GCCn_nnn'nGGC

Cuts at:      0      78     351 
   Size:         78     273
 
BlpI GC'TnA_GC

Cuts at:      0     130     351 
   Size:        130     221
 
Bme1580I G_kGCm'C

Cuts at:      0      74     115     351 
   Size:         74      41     236

  Fragments arranged by size: 

                 236      74      41
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     102     351 
   Size:        102     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     290     351 
   Size:         31     259      61

  Fragments arranged by size: 

                 259      61      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     351 
   Size:        261      90
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     351 
   Size:        253      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     226     256     351 
   Size:        226      30      95

  Fragments arranged by size: 

                 226      95      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     250     351 
   Size:        208      42     101

  Fragments arranged by size: 

                 208     101      42
 
BseYI C'CCAG_C

Cuts at:      0      95     351 
   Size:         95     256
 
BsiHKAI G_wGCw'C

Cuts at:      0     115     306     351 
   Size:        115     191      45

  Fragments arranged by size: 

                 191     115      45
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     115     306     351 
   Size:         74      41     191      45

  Fragments arranged by size: 

                 191      74      45      41
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     229     330     351 
   Size:        229     101      21

  Fragments arranged by size: 

                 229     101      21
 
BstYI r'GATC_y

Cuts at:      0      26     351 
   Size:         26     325
 
BtgI C'CryG_G

Cuts at:      0      59     351 
   Size:         59     292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     290     351 
   Size:         31     259      61

  Fragments arranged by size: 

                 259      61      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70      99     351 
   Size:         70      29     252

  Fragments arranged by size: 

                 252      70      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     351 
   Size:        226      32      93

  Fragments arranged by size: 

                 226      93      32
 
NcoI C'CATG_G

Cuts at:      0      59     351 
   Size:         59     292
 
PpuMI rG'GwC_Cy

Cuts at:      0      99     351 
   Size:         99     252
 
PspOMI G'GGCC_C

Cuts at:      0      70     351 
   Size:         70     281
 
PstI C_TGCA'G

Cuts at:      0     184     351 
   Size:        184     167
 
SanDI GG'GwC_CC

Cuts at:      0      99     351 
   Size:         99     252
 
SbfI CC_TGCA'GG

Cuts at:      0     184     351 
   Size:        184     167
 
SfcI C'TryA_G

Cuts at:      0     180     351 
   Size:        180     171
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      78     351 
   Size:         78     273
 
SmlI C'TyrA_G

Cuts at:      0     276     351 
   Size:        276      75
 
StuI AGG'CCT

Cuts at:      0     226     351 
   Size:        226     125
 
StyI C'CwwG_G

Cuts at:      0      59     169     351 
   Size:         59     110     182

  Fragments arranged by size: 

                 182     110      59
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     156     351 
   Size:        156     195
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      37      92     351 
   Size:         37      55     259

  Fragments arranged by size: 

                 259      55      37
 

 Enzymes that do cut:

     AlfI     ApaI    ApaLI     BanI    BanII     BglI     BlpI Bme1580I
     BmrI     BpmI    BpuEI     BsaI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BstXI    BstYI     BtgI  Eco57MI EcoO109I    Hin4I     NcoI
    PpuMI   PspOMI     PstI    SanDI     SbfI     SfcI     SfiI     SmlI
     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     ScaI    SexAI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI