Restriction map for
pFN21AE6566
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MslI MspA1I NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 403 GTATAC GT^[AC][GT]AC
AccII 66 CGCG CG^CG
AfaI 77 GTAC GT^AC
AlwNI 51 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 365 CTCGAG C^[CT]CG[GA]G
AvaII 69 GGACC G^G[AT]CC
BamHI 120 GGATCC G^GATCC
BfaI 149 CTAG C^TAG
BmyI 315 GGGCAC G[AGT]GC[ACT]^C
BpmI 279 CTCCAG CTGGAG(16/14)
BsaI 331 GAGACC GGTCTC(1/5)
BsmAI 331 GAGAC GTCTC(1/5)
BsoBI 365 CTCGAG C^[CT]CG[GA]G
Bsp1286I 315 GGGCAC G[AGT]GC[ACT]^C
Bst1107I 403 GTATAC GTA^TAC
BstAPI 99 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 240 CATCC GGATG(2/0)
BstUI 66 CGCG CG^CG
BstZ17I 403 GTATAC GTA^TAC
CfoI 274 GCGC GCG^C
Cfr13I 69 GGACC G^G[ACGT]CC
EcoT14I 80 CCAAGG C^C[AT][AT]GG
FokI 240 CATCC GGATG(9/13)
HaeII 273 GGCGCT [GA]GCGC^[CT]
HapII 23 CCGG C^CGG
HhaI 274 GCGC GCG^C
HinP1I 274 GCGC G^CGC
HpaII 23 CCGG C^CGG
MaeI 149 CTAG C^TAG
MfeI 309 CAATTG C^AATTG
MseI 547 TTAA T^TAA
MspI 23 CCGG C^CGG
MunI 309 CAATTG C^AATTG
MvnI 66 CGCG CG^CG
MwoI 99 GCAGACCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 105 CATG CATG^
PaeR7I 365 CTCGAG C^TCGAG
PleI 437 GAGTC GAGTC(4/5)
RsaI 77 GTAC GT^AC
Sau96I 69 GGACC G^G[ACGT]CC
StuI 477 AGGCCT AGG^CCT
StyI 80 CCAAGG C^C[AT][AT]GG
TaqI 366 TCGA T^CGA
Tru9I 547 TTAA T^TAA
Tsp45I 132 GTCAC ^GT[GC]AC
TthHB8I 366 TCGA T^CGA
XhoI 365 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 442 AAATTT [GA]^AATT[CT]
518 AAATTT
AluI 288 AGCT AG^CT
299 AGCT
ApoI 442 AAATTT [GA]^AATT[CT]
518 AAATTT
BsaJI 80 CCAAGG C^C[ACGT][ACGT]GG
269 CCTGGG
BssKI 269 CCTGG ^CC[ACGT]GG
480 CCTGG
BstNI 269 CCTGG CC^[AT]GG
480 CCTGG
BstYI 120 GGATCC [GA]^GATC[CT]
495 AGATCC
DdeI 49 CTCAG C^T[ACGT]AG
125 CTAAG
DpnII 121 GATC ^GATC
496 GATC
EcoRII 269 CCTGG ^CC[AT]GG
480 CCTGG
HgaI 64 GACGC GACGC(5/10)
165 GACGC
MaeIII 132 GTCAC ^GT[ACGT]AC
341 GTTAC
MboI 121 GATC ^GATC
496 GATC
MflI 120 GGATCC [GA]^GATC[CT]
495 AGATCC
MvaI 269 CCTGG CC^[AT]GG
480 CCTGG
NdeII 121 GATC ^GATC
496 GATC
NlaIV 69 GGACCC GG[ACGT]^[ACGT]CC
120 GGATCC
Sau3AI 121 GATC ^GATC
496 GATC
ScrFI 269 CCTGG CC^[ACGT]GG
480 CCTGG
SmlI 295 CTTGAG C^T[CT][GA]AG
365 CTCGAG
TfiI 371 GATTC G^A[AT]TC
525 GAATC
XhoII 120 GGATCC [GA]^GATC[CT]
495 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AlwI BbvI BsiYI BslI
BsrI Fnu4HI HaeIII HinfI ItaI
MboII MnlI TseI Tsp509I TspRI