################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AvrII BamHI BanII BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI HincII HindII HindIII HpaI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 549 GGTACC G^GTACC AcsI 139 GAATTT [GA]^AATT[CT] AgeI 552 ACCGGT A^CCGGT ApoI 139 GAATTT [GA]^AATT[CT] Asp700I 3 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 549 GGTACC G^GTACC AviII 332 TGCGCA TGC^GCA BalI 479 TGGCCA TGG^CCA BbeI 301 GGCGCC GGCGC^C BbvCI 544 GCTGAGG CCTCAGC(-5/-2) BglII 338 AGATCT A^GATCT BsaI 507 GGTCTC GGTCTC(1/5) BsaWI 552 ACCGGT [AT]^CCGG[AT] BstNI 279 CCTGG CC^[AT]GG BstYI 338 AGATCT [GA]^GATC[CT] DdeI 545 CTGAG C^T[ACGT]AG EaeI 479 TGGCCA [CT]^GGCC[GA] EcoRII 279 CCTGG ^CC[AT]GG EcoT14I 134 CCAAGG C^C[AT][AT]GG FspI 332 TGCGCA TGC^GCA HaeII 301 GGCGCC [GA]GCGC^[CT] HinfI 384 GATTC G^A[ACGT]TC HphI 435 GGTGA GGTGA(8/7) KasI 301 GGCGCC G^GCGCC KpnI 549 GGTACC GGTAC^C MaeII 204 ACGT A^CGT MaeIII 292 GTGAC ^GT[ACGT]AC MflI 338 AGATCT [GA]^GATC[CT] MluNI 479 TGGCCA TGG^CCA MscI 479 TGGCCA TGG^CCA MvaI 279 CCTGG CC^[AT]GG NaeI 347 GCCGGC GCC^GGC NarI 301 GGCGCC GG^CGCC NgoMIV 347 GCCGGC G^CCGGC NspI 329 GCATGC [GA]CATG^[CT] PflMI 75 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 552 ACCGGT A^CCGGT SexAI 278 ACCTGGT A^CC[AT]GGT SfaNI 541 GATGC GCATC(5/9) SfoI 301 GGCGCC GGC^GCC SphI 329 GCATGC GCATG^C SspI 472 AATATT AAT^ATT StyI 134 CCAAGG C^C[AT][AT]GG TfiI 384 GATTC G^A[AT]TC Tsp45I 292 GTGAC ^GT[GC]AC Van91I 75 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 338 AGATCT [GA]^GATC[CT] XmnI 3 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 325 CCGC CCGC(-3/-1) 226 GCGG AcyI 151 GACGCC G[GA]^CG[CT]C 301 GGCGCC AfaI 420 GTAC GT^AC 550 GTAC AluI 242 AGCT AG^CT 495 AGCT BbvI 493 GCAGC GCAGC(8/12) 243 GCTGC BcnI 296 CCGGG CC^[GC]GG 516 CCGGG BsaHI 151 GACGCC G[GA]^CG[CT]C 301 GGCGCC BsaJI 134 CCAAGG C^C[ACGT][ACGT]GG 296 CCGGGG BseRI 466 GAGGAG GAGGAG(10/8) 565 GAGGAG BsmAI 175 GAGAC GTCTC(1/5) 508 GTCTC BsmFI 518 GGGAC GGGAC(10/14) 558 GGGAC BsrFI 347 GCCGGC [GA]^CCGG[CT] 552 ACCGGT BsrI 180 ACTGG ACTGG(1/-1) 257 ACTGG Cfr10I 347 GCCGGC [GA]^CCGG[CT] 552 ACCGGT DpnII 96 GATC ^GATC 339 GATC HaeIII 409 GGCC GG^CC 480 GGCC HgaI 151 GACGC GACGC(5/10) 223 GACGC Hin1I 151 GACGCC G[GA]^CG[CT]C 301 GGCGCC MboI 96 GATC ^GATC 339 GATC MboII 186 GAAGA GAAGA(8/7) 341 TCTTC MslI 24 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 285 CATTTCTGTG NciI 296 CCGGG CC^[GC]GG 516 CCGGG NdeII 96 GATC ^GATC 339 GATC RsaI 420 GTAC GT^AC 550 GTAC Sau3AI 96 GATC ^GATC 339 GATC TseI 243 GCTGC G^C[AT]GC 493 GCAGC Tsp509I 140 AATT ^AATT 535 AATT TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^ 256 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII BanI BsiYI BslI BssKI BstUI Cac8I CfoI Fnu4HI HapII HhaI HinP1I HpaII ItaI MnlI MspI MvnI MwoI NlaIII NlaIV ScrFI