################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BfaI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1621 GTCGAC GT^[AC][GT]AC AccII 455 CGCG CG^CG AcsI 1772 AAATTC [GA]^AATT[CT] AflII 1285 CTTAAG C^TTAAG AgeI 699 ACCGGT A^CCGGT AhdI 1169 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 1772 AAATTC [GA]^AATT[CT] AspEI 1169 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 1745 GAGCCC G[GA]GC[CT]^C BbvCI 720 GCTGAGG CCTCAGC(-5/-2) BcnI 5 CCCGG CC^[GC]GG BfrI 1285 CTTAAG C^TTAAG BsaI 1336 GGTCTC GGTCTC(1/5) BsaWI 699 ACCGGT [AT]^CCGG[AT] BsmI 1917 GAATGC GAATGC(1/-1) BspMI 1294 GCAGGT ACCTGC(4/8) BsrFI 699 ACCGGT [GA]^CCGG[CT] BssSI 1157 CACGAG CACGAG(-5/-1) BstEII 1549 GGTGACC G^GT[ACGT]ACC BstPI 1549 GGTGACC G^GT[ACGT]ACC BstUI 455 CGCG CG^CG Cfr10I 699 ACCGGT [GA]^CCGG[CT] DraI 1975 TTTAAA TTT^AAA DraIII 128 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 974 CCATGG C^C[GA][CT]GG EaeI 66 CGGCCA [CT]^GGCC[GA] Eam1105I 1169 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 676 GAAGAG CTCTTC(1/4) EcoNI 577 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1549 GGTGACC G^GT[ACGT]ACC EcoRV 1672 GATATC GAT^ATC EcoT14I 974 CCATGG C^C[AT][AT]GG FauI 1828 CCCGC CCCGC(4/6) HgaI 1194 GACGC GACGC(5/10) Ksp632I 676 GAAGAG CTCTTC(1/4) MaeII 2001 ACGT A^CGT MspA1I 107 CAGCTG C[AC]G^C[GT]G MvnI 455 CGCG CG^CG NciI 5 CCCGG CC^[GC]GG NcoI 974 CCATGG C^CATGG NlaIV 1708 GGCCCC GG[ACGT]^[ACGT]CC NruI 454 TCGCGA TCG^CGA PinAI 699 ACCGGT A^CCGGT PvuII 107 CAGCTG CAG^CTG SalI 1621 GTCGAC G^TCGAC SapI 676 GAAGAGC GCTCTTC(1/4) ScaI 820 AGTACT AGT^ACT SgrAI 698 CACCGGTG C[GA]^CCGG[CT]G StyI 974 CCATGG C^C[AT][AT]GG XcmI 210 CCATCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 1224 GGATC GGATC(4/5) 1498 GGATC AseI 300 ATTAAT AT^TAAT 1023 ATTAAT AsnI 300 ATTAAT AT^TAAT 1023 ATTAAT Asp700I 307 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1246 GAAAGGTTTC AvaII 586 GGACC G^G[AT]CC 1176 GGTCC BclI 228 TGATCA T^GATCA 255 TGATCA BglII 177 AGATCT A^GATCT 1363 AGATCT BmyI 1276 GGGCAC G[AGT]GC[ACT]^C 1745 GAGCCC BsaBI 52 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1668 GATGGATATC BsaJI 974 CCATGG C^C[ACGT][ACGT]GG 1079 CCCAGG BsgI 334 GTGCAG GTGCAG(16/14) 373 GTGCAG Bsp1286I 1276 GGGCAC G[AGT]GC[ACT]^C 1745 GAGCCC BsrDI 606 CATTGC GCAATG(2/0) 1564 GCAATG FbaI 228 TGATCA T^GATCA 255 TGATCA HapII 6 CCGG C^CGG 700 CCGG HincII 102 GTCAAC GT[CT]^[GA]AC 1621 GTCGAC HindII 102 GTCAAC GT[CT]^[GA]AC 1621 GTCGAC HindIII 911 AAGCTT A^AGCTT 1727 AAGCTT HpaII 6 CCGG C^CGG 700 CCGG MamI 52 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1668 GATGGATATC MspI 6 CCGG C^CGG 700 CCGG NdeI 835 CATATG CA^TATG 923 CATATG PleI 1169 GACTC GAGTC(4/5) 892 GAGTC PshBI 300 ATTAAT AT^TAAT 1023 ATTAAT SspI 297 AATATT AAT^ATT 1765 AATATT TaqI 21 TCGA T^CGA 1622 TCGA TthHB8I 21 TCGA T^CGA 1622 TCGA XmnI 307 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1246 GAAAGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI BbvI BpmI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NsiI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII