(Circular) (Six-base) MAPSORT of: pFN21AE5782.seq Check: 2480 from: 1 to: 6757 pFN21AE5782 6757 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3627 4100 1937 Size: 1690 473 4594 Fragments arranged by size: 4594 1690 473 Acc65I G'GTAC_C Cuts at: 4078 4078 Size: 6757 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2574 4820 6204 6472 1632 Size: 710 232 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 710 268 232 AflII C'TTAA_G Cuts at: 820 1017 3290 820 Size: 197 2273 4287 Fragments arranged by size: 4287 2273 197 AflIII A'CryG_T Cuts at: 1203 5656 6633 1203 Size: 4453 977 1327 Fragments arranged by size: 4453 1327 977 AgeI A'CCGG_T Cuts at: 2704 5588 2704 Size: 2884 3873 AhdI GACnn_n'nnGTC Cuts at: 3179 5486 3179 Size: 2307 4450 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 4500 6072 4500 Size: 1572 5185 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 3777 4066 4292 4444 6553 1493 Size: 2284 289 226 152 2109 1697 Fragments arranged by size: 2284 2109 1697 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 2306 3029 5311 160 Size: 872 1274 723 2282 1606 Fragments arranged by size: 2282 1606 1274 872 723 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 4082 1870 Size: 73 2139 4545 Fragments arranged by size: 4545 2139 73 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4078 5533 618 Size: 325 217 627 2291 1455 1842 Fragments arranged by size: 2291 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3754 4076 1391 Size: 2363 322 4072 Fragments arranged by size: 4072 2363 322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5786 BbvCI CC'TCA_GC Cuts at: 2726 2726 Size: 6757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4948 4982 1903 Size: 34 3011 34 3678 Fragments arranged by size: 3678 3011 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4649 5859 4649 Size: 1210 5547 BclI T'GATC_A Cuts at: 1548 2233 2260 1548 Size: 685 27 6045 Fragments arranged by size: 6045 685 27 BfrBI ATG'CAT Cuts at: 2881 2933 3926 2881 Size: 52 993 5712 Fragments arranged by size: 5712 993 52 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5368 136 Size: 107 122 71 631 605 3696 1525 Fragments arranged by size: 3696 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2182 3368 6752 2182 Size: 1186 3384 2187 Fragments arranged by size: 3384 2187 1186 BlpI GC'TnA_GC Cuts at: 4174 4174 Size: 6757 Bme1580I G_kGCm'C Cuts at: 1790 3285 4818 5974 1790 Size: 1495 1533 1156 2573 Fragments arranged by size: 2573 1533 1495 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3640 4488 5446 Size: 416 53 83 399 24 2223 848 958 Cuts at: 5446 442 Size: 1753 Fragments arranged by size: 2223 1753 958 848 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2562 2777 3024 3171 5417 1239 Size: 1323 215 247 147 2246 2579 Fragments arranged by size: 2579 2246 1323 247 215 147 Bpu10I CC'TnA_GC Cuts at: 2726 2726 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3983 4882 5747 6045 2651 Size: 1332 899 865 298 3363 Fragments arranged by size: 3363 1332 899 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3347 5420 882 Size: 617 1848 2073 2219 Fragments arranged by size: 2219 2073 1848 617 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 2061 3677 6751 2061 Size: 1616 3074 2067 Fragments arranged by size: 3074 2067 1616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4564 4946 5581 275 Size: 53 83 186 936 3031 382 635 1451 Fragments arranged by size: 3031 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2704 5190 5588 5862 6009 6420 2704 Size: 2486 398 274 147 411 3041 Fragments arranged by size: 3041 2486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2274 2304 1178 Size: 30 165 30 871 30 5631 Fragments arranged by size: 5631 871 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6757 BseYI C'CCAG_C Cuts at: 2698 2816 3444 3988 5960 6456 2698 Size: 118 628 544 1972 496 2999 Fragments arranged by size: 2999 1972 628 544 496 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2399 1163 Size: 573 624 39 5521 Fragments arranged by size: 5521 624 573 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 4968 5996 1540 Size: 275 186 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4818 4903 5974 4818 Size: 85 1071 5601 Fragments arranged by size: 5601 1071 85 BsmI GAATG_Cn' Cuts at: 3928 4268 4361 3928 Size: 340 93 6324 Fragments arranged by size: 6324 340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3285 3754 4076 4818 4903 5974 1391 Size: 399 1495 469 322 742 85 1071 2174 Fragments arranged by size: 2174 1495 1071 742 469 399 322 85 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 4646 6376 4646 Size: 1730 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3290 4112 844 Size: 2446 822 3489 Fragments arranged by size: 3489 2446 822 BsrBI CCG'CTC Cuts at: 1394 1823 4644 1394 Size: 429 2821 3507 Fragments arranged by size: 3507 2821 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 3576 5252 5426 65 Size: 1183 1362 966 1676 174 1396 Fragments arranged by size: 1676 1396 1362 1183 966 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2704 5401 5588 1700 Size: 110 894 2697 187 2869 Fragments arranged by size: 2869 2697 894 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 3162 4817 5829 3162 Size: 1655 1012 4090 Fragments arranged by size: 4090 1655 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6757 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 3554 1184 Size: 2370 4387 BstYI r'GATC_y Cuts at: 1972 2182 3368 3503 4087 4454 4839 4856 6308 Size: 210 1186 135 584 367 385 17 1452 Cuts at: 6308 6394 6406 6752 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1186 584 385 367 346 210 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 4291 Fragments arranged by size: 4291 975 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2442 487 Size: 683 56 1216 4802 Fragments arranged by size: 4802 1216 683 56 BtsI GCAGTG_nn' Cuts at: 4273 5067 5087 4273 Size: 794 20 5943 Fragments arranged by size: 5943 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 3982 4061 3982 Size: 79 6678 DraIII CAC_nnn'GTG Cuts at: 2138 2138 Size: 6757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 4204 5093 Size: 54 1425 50 275 18 241 2133 889 Cuts at: 5093 6722 8 Size: 1629 43 Fragments arranged by size: 2133 1629 1425 889 275 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2676 4687 1353 Size: 1323 2011 3423 Fragments arranged by size: 3423 2011 1323 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 3104 5344 5718 5864 387 Size: 1064 138 1176 339 2240 374 146 1280 Fragments arranged by size: 2240 1280 1176 1064 374 339 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2562 2574 2777 3024 3171 4820 Size: 393 710 220 12 203 247 147 1649 Cuts at: 4820 5417 6204 6472 1239 Size: 597 787 268 1524 Fragments arranged by size: 1649 1524 787 710 597 393 268 247 220 203 147 12 EcoNI CCTnn'n_nnAGG Cuts at: 2586 2586 Size: 6757 EcoRI G'AATT_C Cuts at: 4066 4444 4066 Size: 378 6379 EcoRV GAT'ATC Cuts at: 3679 3679 Size: 6757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 2261 Size: 6757 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 5904 1678 Size: 4226 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3165 3197 3488 3520 3741 Size: 32 135 32 1593 32 291 32 221 Cuts at: 3741 3773 5398 5430 5472 5504 1373 Size: 32 1625 32 42 32 2626 Fragments arranged by size: 2626 1625 1593 291 221 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2109 3628 4101 4347 669 Size: 988 281 171 1519 473 246 3079 Fragments arranged by size: 3079 1519 988 473 281 246 171 HindIII A'AGCT_T Cuts at: 748 2916 3732 748 Size: 2168 816 3773 Fragments arranged by size: 3773 2168 816 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 4082 4082 Size: 6757 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5871 6055 1305 Size: 4566 184 2007 Fragments arranged by size: 4566 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4935 Fragments arranged by size: 4935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2019 2811 2886 2932 4715 5074 5233 Size: 648 853 792 75 46 1783 359 159 Cuts at: 5233 518 Size: 2042 Fragments arranged by size: 2042 1783 853 792 648 359 159 75 46 MspA1I CmG'CkG Cuts at: 1606 2114 4173 4850 5998 6243 1606 Size: 508 2059 677 1148 245 2120 Fragments arranged by size: 2120 2059 1148 677 508 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 4291 Fragments arranged by size: 4291 975 939 552 NdeI CA'TA_TG Cuts at: 387 2841 2929 387 Size: 2454 88 4215 Fragments arranged by size: 4215 2454 88 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NruI TCG'CGA Cuts at: 2461 2461 Size: 6757 NsiI A_TGCA'T Cuts at: 2883 2935 3928 2883 Size: 52 993 5712 Fragments arranged by size: 5712 993 52 NspI r_CATG'y Cuts at: 1132 1229 4115 5660 1132 Size: 97 2886 1545 2229 Fragments arranged by size: 2886 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6757 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4826 4858 6339 6371 4826 Size: 32 1481 32 5212 Fragments arranged by size: 5212 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PstI C_TGCA'G Cuts at: 830 4109 830 Size: 3279 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 PvuII CAG'CTG Cuts at: 2114 2114 Size: 6757 SalI G'TCGA_C Cuts at: 1936 3626 4099 1936 Size: 1690 473 4594 Fragments arranged by size: 4594 1690 473 SapI GCTCTTCn'nnn_ Cuts at: 2676 2676 Size: 6757 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 2827 5005 6717 2827 Size: 2178 1712 2867 Fragments arranged by size: 2867 2178 1712 SfcI C'TryA_G Cuts at: 826 1045 2079 2415 3938 4105 5240 5921 6112 Size: 219 1034 336 1523 167 1135 681 191 Cuts at: 6112 826 Size: 1471 Fragments arranged by size: 1523 1471 1135 1034 681 336 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 2704 1700 Size: 1004 5753 SmaI CCC'GGG Cuts at: 1872 4084 1872 Size: 2212 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2630 3290 3962 4861 5762 6024 Size: 197 926 687 660 672 899 901 262 Cuts at: 6024 820 Size: 1553 Fragments arranged by size: 1553 926 901 899 687 672 660 262 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 4115 1132 Size: 2983 3774 SspI AAT'ATT Cuts at: 5 52 2304 3772 4681 5 Size: 47 2252 1468 909 2081 Fragments arranged by size: 2252 2081 1468 909 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6757 StyI C'CwwG_G Cuts at: 513 1065 2004 2979 4196 513 Size: 552 939 975 1217 3074 Fragments arranged by size: 3074 1217 975 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3573 4784 4801 4954 5139 5584 325 Size: 1255 1993 1211 17 153 185 445 1498 Fragments arranged by size: 1993 1498 1255 1211 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2825 5003 6715 Size: 274 80 33 51 1443 848 2178 1712 Cuts at: 6715 96 Size: 138 Fragments arranged by size: 2178 1712 1443 848 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 2520 2552 3786 3818 6717 6749 2274 Size: 32 214 32 1234 32 2899 32 2282 Fragments arranged by size: 2899 2282 1234 214 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2222 2222 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6757 XmaI C'CCGG_G Cuts at: 1870 4082 1870 Size: 2212 4545 XmnI GAAnn'nnTTC Cuts at: 2316 3255 4886 2316 Size: 939 1631 4187 Fragments arranged by size: 4187 1631 939 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI