################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BalI BamHI BbeI BcgI BclI BglI BglII BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstYI BstZ17I Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SspI SwaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 2055 CGCG CG^CG AflII 1414 CTTAAG C^TTAAG AlwI 684 GATCC GGATC(4/5) Aor51HI 1785 AGCGCT AGC^GCT AspI 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2077 CCCGAG C^[CT]CG[GA]G AvrII 394 CCTAGG C^CTAGG BbrPI 905 CACGTG CAC^GTG BbsI 2071 GAAGAC GAAGAC(2/6) BciVI 523 GGATAC GTATCC(6/5) BfrI 1414 CTTAAG C^TTAAG BlnI 394 CCTAGG C^CTAGG BlpI 1711 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1711 GCTAAGC GC^T[ACGT]AGC BpuAI 2071 GAAGAC GAAGAC(2/6) BsaAI 905 CACGTG [CT]AC^GT[GA] BseRI 1546 CTCCTC GAGGAG(10/8) BsmBI 1197 CGTCTC CGTCTC(1/5) BsoBI 2077 CCCGAG C^[CT]CG[GA]G BspHI 1931 TCATGA T^CATGA BspMI 662 ACCTGC ACCTGC(4/8) BsrDI 281 CATTGC GCAATG(2/0) BstAPI 1225 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 55 TTCGAA TT^CGAA BstEII 1381 GGTCACC G^GT[ACGT]ACC BstPI 1381 GGTCACC G^GT[ACGT]ACC BstUI 2055 CGCG CG^CG BstXI 954 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1711 GCTAAGC GC^T[ACGT]AGC DraI 301 TTTAAA TTT^AAA DsaI 2054 CCGCGG C^C[GA][CT]GG Eco47III 1785 AGCGCT AGC^GCT EcoO65I 1381 GGTCACC G^GT[ACGT]ACC EcoRI 259 GAATTC G^AATTC FauI 1881 GCGGG CCCGC(4/6) HaeII 1785 AGCGCT [GA]GCGC^[CT] HincII 613 GTTGAC GT[CT]^[GA]AC HindII 613 GTTGAC GT[CT]^[GA]AC KspI 2054 CCGCGG CCGC^GG MaeII 906 ACGT A^CGT MslI 1791 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 2055 CGCG CG^CG NspV 55 TTCGAA TT^CGAA PflFI 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 905 CACGTG CAC^GTG PmlI 905 CACGTG CAC^GTG RcaI 1931 TCATGA T^CATGA SacII 2054 CCGCGG CCGC^GG SapI 1926 GCTCTTC GCTCTTC(1/4) SbfI 1020 CCTGCAGG CCTGCA^GG SfuI 55 TTCGAA TT^CGAA SphI 1988 GCATGC GCATG^C Sse8387I 1020 CCTGCAGG CCTGCA^GG StuI 795 AGGCCT AGG^CCT TaqI 56 TCGA T^CGA Tth111I 513 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 56 TCGA T^CGA XbaI 186 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 149 AAATTT [GA]^AATT[CT] 259 GAATTC AflIII 488 ACATGT A^C[GA][CT]GT 1628 ACATGT ApoI 149 AAATTT [GA]^AATT[CT] 259 GAATTC BanI 745 GGCACC G^G[CT][GA]CC 1578 GGCACC BcnI 890 CCCGG CC^[GC]GG 1978 CCGGG BsaI 914 GAGACC GGTCTC(1/5) 1102 GAGACC BsmI 865 GCATTC GAATGC(1/-1) 1160 GCATTC BspLU11I 488 ACATGT A^CATGT 1628 ACATGT CfoI 1786 GCGC GCG^C 1872 GCGC HapII 891 CCGG C^CGG 1978 CCGG HhaI 1786 GCGC GCG^C 1872 GCGC HinP1I 1786 GCGC G^CGC 1872 GCGC HindIII 606 AAGCTT A^AGCTT 1714 AAGCTT HpaII 891 CCGG C^CGG 1978 CCGG MspI 891 CCGG C^CGG 1978 CCGG NciI 890 CCCGG CC^[GC]GG 1978 CCGGG PpuMI 1836 GGGACCC [GA]G^G[AT]CC[CT] 1949 AGGTCCT SacI 1089 GAGCTC GAGCT^C 1543 GAGCTC SexAI 1172 ACCTGGT A^CC[AT]GGT 1967 ACCAGGT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AlwNI AocI Asp700I AspHI AvaII BanII BbvCI BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DraIII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XmnI