(Circular) (Six-base) MAPSORT of: pFN21AE5784.seq Check: 2265 from: 1 to: 6799 pFN21AE5784 6799 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4609 278 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4142 1937 Size: 2205 4594 Acc65I G'GTAC_C Cuts at: 4120 4120 Size: 6799 AclI AA'CG_TT Cuts at: 1563 4926 5299 1563 Size: 3363 373 3063 Fragments arranged by size: 3363 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2004 2060 2696 3155 4862 6246 6514 1632 Size: 372 56 636 459 1707 1384 268 1917 Fragments arranged by size: 1917 1707 1384 636 459 372 268 56 AfeI AGC'GCT Cuts at: 3792 3792 Size: 6799 AflII C'TTAA_G Cuts at: 820 1017 3419 820 Size: 197 2402 4200 Fragments arranged by size: 4200 2402 197 AflIII A'CryG_T Cuts at: 1203 2493 3633 5698 6675 1203 Size: 1290 1140 2065 977 1327 Fragments arranged by size: 2065 1327 1290 1140 977 AgeI A'CCGG_T Cuts at: 5630 5630 Size: 6799 AhdI GACnn_n'nnGTC Cuts at: 5528 5528 Size: 6799 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2774 1184 Size: 1590 5209 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2800 2832 2800 Size: 32 6767 AlwNI CAG_nnn'CTG Cuts at: 2866 3025 3178 3236 3752 3820 4542 6114 2866 Size: 159 153 58 516 68 722 1572 3551 Fragments arranged by size: 3551 1572 722 516 159 153 68 58 ApaLI G'TGCA_C Cuts at: 4856 6012 4856 Size: 1156 5643 ApoI r'AATT_y Cuts at: 1493 2154 2264 4108 4334 4486 6595 1493 Size: 661 110 1844 226 152 2109 1697 Fragments arranged by size: 2109 1844 1697 661 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 5353 160 Size: 872 4321 1606 Fragments arranged by size: 4321 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 AvaI C'yCGr_G Cuts at: 1870 1943 4082 4124 1870 Size: 73 2139 42 4545 Fragments arranged by size: 4545 2139 73 42 AvrII C'CTAG_G Cuts at: 2399 2399 Size: 6799 BamHI G'GATC_C Cuts at: 4129 4496 4129 Size: 367 6432 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2750 3583 4120 5575 618 Size: 325 217 627 963 833 537 1455 1842 Fragments arranged by size: 1842 1455 963 833 627 537 325 217 BanII G_rGCy'C Cuts at: 1391 3098 3552 3737 4118 1391 Size: 1707 454 185 381 4072 Fragments arranged by size: 4072 1707 454 381 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4083 928 Size: 971 2184 3644 Fragments arranged by size: 3644 2184 971 BbvCI CC'TCA_GC Cuts at: 3295 3506 3588 3295 Size: 211 82 6506 Fragments arranged by size: 6506 211 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4990 5024 1903 Size: 34 3053 34 3678 Fragments arranged by size: 3678 3053 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2522 4691 5901 2522 Size: 2169 1210 3420 Fragments arranged by size: 3420 2169 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6799 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5410 136 Size: 107 122 71 631 605 3738 1525 Fragments arranged by size: 3738 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6794 6794 Size: 6799 BlpI GC'TnA_GC Cuts at: 3717 4216 3717 Size: 499 6300 Bme1580I G_kGCm'C Cuts at: 1790 4860 6016 1790 Size: 3070 1156 2573 Fragments arranged by size: 3070 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6799 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3406 3740 4530 Size: 416 53 83 399 24 1989 334 790 Cuts at: 4530 5488 442 Size: 958 1753 Fragments arranged by size: 1989 1753 958 790 416 399 334 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6799 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3571 3571 Size: 6799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2327 2647 2988 3668 5459 1239 Size: 1046 42 320 341 680 1791 2579 Fragments arranged by size: 2579 1791 1046 680 341 320 42 Bpu10I CC'TnA_GC Cuts at: 2705 3295 3506 3588 2705 Size: 590 211 82 5916 Fragments arranged by size: 5916 590 211 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 2631 4924 5789 6087 2243 Size: 388 2293 865 298 2955 Fragments arranged by size: 2955 2293 865 388 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2913 3101 5462 882 Size: 617 1414 188 2361 2219 Fragments arranged by size: 2361 2219 1414 617 188 BsaAI yAC'GTr Cuts at: 493 1204 2912 4590 493 Size: 711 1708 1678 2702 Fragments arranged by size: 2702 1708 1678 711 BsaBI GATnn'nnATC Cuts at: 6793 6793 Size: 6799 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4606 4988 5623 275 Size: 53 83 186 936 3073 382 635 1451 Fragments arranged by size: 3073 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5232 5630 5904 6051 6462 5232 Size: 398 274 147 411 5569 Fragments arranged by size: 5569 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3186 3216 3666 3696 1178 Size: 30 165 30 1783 30 450 30 4281 Fragments arranged by size: 4281 1783 450 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3542 1186 Size: 2356 4443 BseYI C'CCAG_C Cuts at: 2923 3242 6002 6498 2923 Size: 319 2760 496 3224 Fragments arranged by size: 3224 2760 496 319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3141 3749 3890 1163 Size: 573 1405 608 141 4072 Fragments arranged by size: 4072 1405 608 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4249 5010 6038 1540 Size: 275 186 2248 761 1028 2301 Fragments arranged by size: 2301 2248 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2909 3061 3098 3552 4860 4945 6016 2909 Size: 152 37 454 1308 85 1071 3692 Fragments arranged by size: 3692 1308 1071 454 152 85 37 BsmI GAATG_Cn' Cuts at: 2870 3165 4310 4403 2870 Size: 295 1145 93 5266 Fragments arranged by size: 5266 1145 295 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3208 3208 Size: 6799 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2909 3061 3098 3552 3737 4118 4860 Size: 399 1119 152 37 454 185 381 742 Cuts at: 4860 4945 6016 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1119 1071 742 454 399 381 185 152 85 37 BspEI T'CCGG_A Cuts at: 6462 6462 Size: 6799 BspHI T'CATG_A Cuts at: 3936 4688 6418 3936 Size: 752 1730 4317 Fragments arranged by size: 4317 1730 752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 4154 844 Size: 1832 1478 3489 Fragments arranged by size: 3489 1832 1478 BsrBI CCG'CTC Cuts at: 1394 1823 4686 1394 Size: 429 2863 3507 Fragments arranged by size: 3507 2863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2285 5294 5468 65 Size: 1183 1037 3009 174 1396 Fragments arranged by size: 3009 1396 1183 1037 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5443 5630 1700 Size: 110 3633 187 2869 Fragments arranged by size: 3633 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2992 3630 3636 96 Size: 2896 638 6 3259 Fragments arranged by size: 3259 2896 638 6 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6799 BssSI C'ACGA_G Cuts at: 4859 5871 4859 Size: 1012 5787 BstAPI GCAn_nnn'nTGC Cuts at: 794 3236 794 Size: 2442 4357 BstBI TT'CG_AA Cuts at: 2061 5603 2061 Size: 3542 3257 BstEII G'GTnAC_C Cuts at: 1184 3386 1184 Size: 2202 4597 BstXI CCAn_nnnn'nTGG Cuts at: 2966 2966 Size: 6799 BstYI r'GATC_y Cuts at: 1972 4129 4496 4881 4898 6350 6436 6448 6794 Size: 2157 367 385 17 1452 86 12 346 Cuts at: 6794 1972 Size: 1977 Fragments arranged by size: 2157 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2804 3117 3302 2804 Size: 313 185 6301 Fragments arranged by size: 6301 313 185 BtgI C'CryG_G Cuts at: 513 1065 4059 513 Size: 552 2994 3253 Fragments arranged by size: 3253 2994 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6060 Fragments arranged by size: 6060 683 56 BtsI GCAGTG_nn' Cuts at: 3013 3943 4315 5109 5129 3013 Size: 930 372 794 20 4683 Fragments arranged by size: 4683 930 794 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6799 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6764 DraI TTT'AAA Cuts at: 2308 4103 2308 Size: 1795 5004 DraIII CAC_nnn'GTG Cuts at: 2249 3181 3949 2249 Size: 932 768 5099 Fragments arranged by size: 5099 932 768 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5806 809 Size: 507 4490 1802 Fragments arranged by size: 4490 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4246 5135 6764 Size: 54 1425 50 275 18 2416 889 1629 Cuts at: 6764 8 Size: 43 Fragments arranged by size: 2416 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4246 1537 Size: 275 2434 4090 Fragments arranged by size: 4090 2434 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 3376 3938 4026 4729 1353 Size: 1239 784 562 88 703 3423 Fragments arranged by size: 3423 1239 784 703 562 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3581 3883 5386 5760 5906 387 Size: 1064 138 1992 302 1503 374 146 1280 Fragments arranged by size: 1992 1503 1280 1064 374 302 146 138 EcoICRI GAG'CTC Cuts at: 3096 3550 3096 Size: 454 6345 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2060 2285 2327 2647 2696 2988 Size: 393 372 56 225 42 320 49 292 Cuts at: 2988 3155 3668 4862 5459 6246 6514 1239 Size: 167 513 1194 597 787 268 1524 Fragments arranged by size: 1524 1194 787 597 513 393 372 320 292 268 225 167 56 49 42 EcoO109I rG'GnC_Cy Cuts at: 2943 3022 3842 3955 2943 Size: 79 820 113 5787 Fragments arranged by size: 5787 820 113 79 EcoRI G'AATT_C Cuts at: 2264 4108 4486 2264 Size: 1844 378 4577 Fragments arranged by size: 4577 1844 378 FspI TGC'GCA Cuts at: 5305 5305 Size: 6799 HaeII r_GCGC'y Cuts at: 1678 3794 5946 1678 Size: 2116 2152 2531 Fragments arranged by size: 2531 2152 2116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3186 3218 3278 3310 5440 Size: 32 135 32 1614 32 60 32 2130 Cuts at: 5440 5472 5514 5546 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2130 1614 135 60 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2620 4143 4389 669 Size: 988 281 682 1523 246 3079 Fragments arranged by size: 3079 1523 988 682 281 246 HindIII A'AGCT_T Cuts at: 748 2611 3719 748 Size: 1863 1108 3828 Fragments arranged by size: 3828 1863 1108 HpaI GTT'AAC Cuts at: 4389 4389 Size: 6799 KpnI G_GTAC'C Cuts at: 4124 4124 Size: 6799 MfeI C'AATT_G Cuts at: 4398 4398 Size: 6799 MluI A'CGCG_T Cuts at: 6675 6675 Size: 6799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3783 5913 6097 1305 Size: 2478 2130 184 2007 Fragments arranged by size: 2478 2130 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4977 Fragments arranged by size: 4977 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4757 5116 5275 518 Size: 648 2634 957 359 159 2042 Fragments arranged by size: 2634 2042 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2625 3041 3192 3580 3688 4061 4215 4892 Size: 1019 416 151 388 108 373 154 677 Cuts at: 4892 6040 6285 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1019 677 416 388 373 245 154 151 108 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6247 NdeI CA'TA_TG Cuts at: 387 387 Size: 6799 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6799 NotI GC'GGCC_GC Cuts at: 4246 4246 Size: 6799 NspI r_CATG'y Cuts at: 1132 1229 2439 2497 3637 3997 4157 5702 1132 Size: 97 1210 58 1140 360 160 1545 2229 Fragments arranged by size: 2229 1545 1210 1140 360 160 97 58 PasI CC'CwG_GG Cuts at: 1348 2693 1348 Size: 1345 5454 PciI A'CATG_T Cuts at: 2493 3633 5698 2493 Size: 1140 2065 3594 Fragments arranged by size: 3594 2065 1140 PflMI CCAn_nnn'nTGG Cuts at: 1108 2450 2774 3752 1108 Size: 1342 324 978 4155 Fragments arranged by size: 4155 1342 978 324 PfoI T'CCnGG_A Cuts at: 2965 3135 2965 Size: 170 6629 PmeI GTTT'AAAC Cuts at: 4103 4103 Size: 6799 PmlI CAC'GTG Cuts at: 2912 2912 Size: 6799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2025 2057 2716 2748 4868 4900 6381 6413 2025 Size: 32 659 32 2120 32 1481 32 2411 Fragments arranged by size: 2411 2120 1481 659 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3842 3955 3842 Size: 113 6686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6799 PsiI TTA'TAA Cuts at: 2423 4369 2423 Size: 1946 4853 PstI C_TGCA'G Cuts at: 830 2763 3030 3379 4151 830 Size: 1933 267 349 772 3478 Fragments arranged by size: 3478 1933 772 349 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6799 PvuII CAG'CTG Cuts at: 2625 3041 3580 3688 2625 Size: 416 539 108 5736 Fragments arranged by size: 5736 539 416 108 SacI G_AGCT'C Cuts at: 3098 3552 3098 Size: 454 6345 SacII CC_GC'GG Cuts at: 4062 4062 Size: 6799 SalI G'TCGA_C Cuts at: 1936 4141 1936 Size: 2205 4594 SanDI GG'GwC_CC Cuts at: 3842 3842 Size: 6799 SapI GCTCTTCn'nnn_ Cuts at: 3938 3938 Size: 6799 SbfI CC_TGCA'GG Cuts at: 3030 4151 3030 Size: 1121 5678 ScaI AGT'ACT Cuts at: 5047 6759 5047 Size: 1712 5087 SexAI A'CCwGG_T Cuts at: 3177 3972 3177 Size: 795 6004 SfcI C'TryA_G Cuts at: 826 1045 2759 3026 3375 4147 5282 5963 6154 Size: 219 1714 267 349 772 1135 681 191 Cuts at: 6154 826 Size: 1471 Fragments arranged by size: 1714 1471 1135 772 681 349 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6799 SmaI CCC'GGG Cuts at: 1872 4126 1872 Size: 2254 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2646 3419 4903 5804 6066 Size: 197 926 279 424 773 1484 901 262 Cuts at: 6066 820 Size: 1553 Fragments arranged by size: 1553 1484 926 901 773 424 279 262 197 SnaBI TAC'GTA Cuts at: 493 4590 493 Size: 4097 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6799 SphI G_CATG'C Cuts at: 1132 3997 4157 1132 Size: 2865 160 3774 Fragments arranged by size: 3774 2865 160 SspI AAT'ATT Cuts at: 5 52 4723 5 Size: 47 4671 2081 Fragments arranged by size: 4671 2081 47 StuI AGG'CCT Cuts at: 1517 2802 1517 Size: 1285 5514 StyI C'CwwG_G Cuts at: 513 1065 2399 2784 3322 3666 3848 4238 513 Size: 552 1334 385 538 344 182 390 3074 Fragments arranged by size: 3074 1334 552 538 390 385 344 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3348 3869 4826 4843 4996 5181 5626 Size: 1255 1768 521 957 17 153 185 445 Cuts at: 5626 325 Size: 1498 Fragments arranged by size: 1768 1498 1255 957 521 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2992 3630 3636 Size: 274 80 33 51 1443 1015 638 6 Cuts at: 3636 5045 6757 96 Size: 1409 1712 138 Fragments arranged by size: 1712 1443 1409 1015 638 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3113 3145 3666 3698 6759 6791 3113 Size: 32 521 32 3061 32 3121 Fragments arranged by size: 3121 3061 521 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2521 1316 Size: 1205 5594 XbaI T'CTAG_A Cuts at: 2191 4135 2191 Size: 1944 4855 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6799 XmaI C'CCGG_G Cuts at: 1870 4124 1870 Size: 2254 4545 XmnI GAAnn'nnTTC Cuts at: 2040 2127 2187 4928 2040 Size: 87 60 2741 3911 Fragments arranged by size: 3911 2741 87 60 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4607 276 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SfiI SfoI SrfI SwaI XcmI