(Linear) (Six-base) MAPSORT of: KIEE5784.seq Check: 2741 from: 1 to: 2094 KIEE5784.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 691 1150 2094 Size: 55 636 459 944 Fragments arranged by size: 944 636 459 55 AfeI AGC'GCT Cuts at: 0 1787 2094 Size: 1787 307 AflII C'TTAA_G Cuts at: 0 1414 2094 Size: 1414 680 AflIII A'CryG_T Cuts at: 0 488 1628 2094 Size: 488 1140 466 Fragments arranged by size: 1140 488 466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 769 2094 Size: 769 1325 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 795 827 2094 Size: 795 32 1267 Fragments arranged by size: 1267 795 32 AlwNI CAG_nnn'CTG Cuts at: 0 861 1020 1173 1231 1747 1815 2094 Size: 861 159 153 58 516 68 279 Fragments arranged by size: 861 516 279 159 153 68 58 ApoI r'AATT_y Cuts at: 0 149 259 2094 Size: 149 110 1835 Fragments arranged by size: 1835 149 110 AvaI C'yCGr_G Cuts at: 0 2077 2094 Size: 2077 17 AvrII C'CTAG_G Cuts at: 0 394 2094 Size: 394 1700 BanI G'GyrC_C Cuts at: 0 745 1578 2094 Size: 745 833 516 Fragments arranged by size: 833 745 516 BanII G_rGCy'C Cuts at: 0 1093 1547 1732 2094 Size: 1093 454 185 362 Fragments arranged by size: 1093 454 362 185 BbsI GAAGACnn'nnnn_ Cuts at: 0 2078 2094 Size: 2078 16 BbvCI CC'TCA_GC Cuts at: 0 1290 1501 1583 2094 Size: 1290 211 82 511 Fragments arranged by size: 1290 511 211 82 BciVI GTATCCnnnnn_n' Cuts at: 0 517 2094 Size: 517 1577 BlpI GC'TnA_GC Cuts at: 0 1712 2094 Size: 1712 382 BmrI ACTGGGnnnn_n' Cuts at: 0 1401 1735 2094 Size: 1401 334 359 Fragments arranged by size: 1401 359 334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1566 2094 Size: 1566 528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 322 642 983 1663 2094 Size: 280 42 320 341 680 431 Fragments arranged by size: 680 431 341 320 280 42 Bpu10I CC'TnA_GC Cuts at: 0 700 1290 1501 1583 2094 Size: 700 590 211 82 511 Fragments arranged by size: 700 590 511 211 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 626 2094 Size: 238 388 1468 Fragments arranged by size: 1468 388 238 BsaI GGTCTCn'nnnn_ Cuts at: 0 908 1096 2094 Size: 908 188 998 Fragments arranged by size: 998 908 188 BsaAI yAC'GTr Cuts at: 0 907 2094 Size: 907 1187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1181 1211 1661 1691 2094 Size: 1181 30 450 30 403 Fragments arranged by size: 1181 450 403 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1537 2094 Size: 1537 557 BseYI C'CCAG_C Cuts at: 0 918 1237 2094 Size: 918 319 857 Fragments arranged by size: 918 857 319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1136 1744 1885 2094 Size: 1136 608 141 209 Fragments arranged by size: 1136 608 209 141 BsiHKAI G_wGCw'C Cuts at: 0 904 1056 1093 1547 2094 Size: 904 152 37 454 547 Fragments arranged by size: 904 547 454 152 37 BsmI GAATG_Cn' Cuts at: 0 865 1160 2094 Size: 865 295 934 Fragments arranged by size: 934 865 295 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1203 2094 Size: 1203 891 Bsp1286I G_dGCh'C Cuts at: 0 904 1056 1093 1547 1732 2094 Size: 904 152 37 454 185 362 Fragments arranged by size: 904 454 362 185 152 37 BspHI T'CATG_A Cuts at: 0 1931 2094 Size: 1931 163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 671 2094 Size: 671 1423 BsrDI GCAATG_nn' Cuts at: 0 280 2094 Size: 280 1814 BsrGI T'GTAC_A Cuts at: 0 987 1625 1631 2094 Size: 987 638 6 463 Fragments arranged by size: 987 638 463 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 1231 2094 Size: 1231 863 BstBI TT'CG_AA Cuts at: 0 56 2094 Size: 56 2038 BstEII G'GTnAC_C Cuts at: 0 1381 2094 Size: 1381 713 BstXI CCAn_nnnn'nTGG Cuts at: 0 961 2094 Size: 961 1133 Bsu36I CC'TnA_GG Cuts at: 0 799 1112 1297 2094 Size: 799 313 185 797 Fragments arranged by size: 799 797 313 185 BtgI C'CryG_G Cuts at: 0 2054 2094 Size: 2054 40 BtsI GCAGTG_nn' Cuts at: 0 1008 1938 2094 Size: 1008 930 156 Fragments arranged by size: 1008 930 156 DraI TTT'AAA Cuts at: 0 303 2094 Size: 303 1791 DraIII CAC_nnn'GTG Cuts at: 0 244 1176 1944 2094 Size: 244 932 768 150 Fragments arranged by size: 932 768 244 150 EarI CTCTTCn'nnn_ Cuts at: 0 587 1371 1933 2021 2094 Size: 587 784 562 88 73 Fragments arranged by size: 784 587 562 88 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1576 1878 2094 Size: 1576 302 216 Fragments arranged by size: 1576 302 216 EcoICRI GAG'CTC Cuts at: 0 1091 1545 2094 Size: 1091 454 549 Fragments arranged by size: 1091 549 454 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 280 322 642 691 983 1150 1663 Size: 55 225 42 320 49 292 167 513 Cuts at: 1663 2094 Size: 431 Fragments arranged by size: 513 431 320 292 225 167 55 49 42 EcoO109I rG'GnC_Cy Cuts at: 0 938 1017 1837 1950 2094 Size: 938 79 820 113 144 Fragments arranged by size: 938 820 144 113 79 EcoRI G'AATT_C Cuts at: 0 259 2094 Size: 259 1835 HaeII r_GCGC'y Cuts at: 0 1789 2094 Size: 1789 305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1181 1213 1273 1305 2094 Size: 1181 32 60 32 789 Fragments arranged by size: 1181 789 60 32 32 HincII GTy'rAC Cuts at: 0 615 2094 Size: 615 1479 HindIII A'AGCT_T Cuts at: 0 606 1714 2094 Size: 606 1108 380 Fragments arranged by size: 1108 606 380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1778 2094 Size: 1778 316 MslI CAynn'nnrTG Cuts at: 0 1795 2094 Size: 1795 299 MspA1I CmG'CkG Cuts at: 0 620 1036 1187 1575 1683 2056 2094 Size: 620 416 151 388 108 373 38 Fragments arranged by size: 620 416 388 373 151 108 38 NspI r_CATG'y Cuts at: 0 434 492 1632 1992 2094 Size: 434 58 1140 360 102 Fragments arranged by size: 1140 434 360 102 58 PasI CC'CwG_GG Cuts at: 0 688 2094 Size: 688 1406 PciI A'CATG_T Cuts at: 0 488 1628 2094 Size: 488 1140 466 Fragments arranged by size: 1140 488 466 PflMI CCAn_nnn'nTGG Cuts at: 0 445 769 1747 2094 Size: 445 324 978 347 Fragments arranged by size: 978 445 347 324 PfoI T'CCnGG_A Cuts at: 0 960 1130 2094 Size: 960 170 964 Fragments arranged by size: 964 960 170 PmlI CAC'GTG Cuts at: 0 907 2094 Size: 907 1187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 20 52 711 743 2094 Size: 20 32 659 32 1351 Fragments arranged by size: 1351 659 32 32 20 PpuMI rG'GwC_Cy Cuts at: 0 1837 1950 2094 Size: 1837 113 144 Fragments arranged by size: 1837 144 113 PsiI TTA'TAA Cuts at: 0 418 2094 Size: 418 1676 PstI C_TGCA'G Cuts at: 0 758 1025 1374 2094 Size: 758 267 349 720 Fragments arranged by size: 758 720 349 267 PvuII CAG'CTG Cuts at: 0 620 1036 1575 1683 2094 Size: 620 416 539 108 411 Fragments arranged by size: 620 539 416 411 108 SacI G_AGCT'C Cuts at: 0 1093 1547 2094 Size: 1093 454 547 Fragments arranged by size: 1093 547 454 SacII CC_GC'GG Cuts at: 0 2057 2094 Size: 2057 37 SanDI GG'GwC_CC Cuts at: 0 1837 2094 Size: 1837 257 SapI GCTCTTCn'nnn_ Cuts at: 0 1933 2094 Size: 1933 161 SbfI CC_TGCA'GG Cuts at: 0 1025 2094 Size: 1025 1069 SexAI A'CCwGG_T Cuts at: 0 1172 1967 2094 Size: 1172 795 127 Fragments arranged by size: 1172 795 127 SfcI C'TryA_G Cuts at: 0 754 1021 1370 2094 Size: 754 267 349 724 Fragments arranged by size: 754 724 349 267 SmlI C'TyrA_G Cuts at: 0 217 641 1414 2094 Size: 217 424 773 680 Fragments arranged by size: 773 680 424 217 SphI G_CATG'C Cuts at: 0 1992 2094 Size: 1992 102 StuI AGG'CCT Cuts at: 0 797 2094 Size: 797 1297 StyI C'CwwG_G Cuts at: 0 394 779 1317 1661 1843 2094 Size: 394 385 538 344 182 251 Fragments arranged by size: 538 394 385 344 251 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1343 1864 2094 Size: 1343 521 230 Fragments arranged by size: 1343 521 230 TatI w'GTAC_w Cuts at: 0 987 1625 1631 2094 Size: 987 638 6 463 Fragments arranged by size: 987 638 463 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1661 1693 2094 Size: 1108 32 521 32 401 Fragments arranged by size: 1108 521 401 32 32 Tth111I GACn'n_nGTC Cuts at: 0 516 2094 Size: 516 1578 XbaI T'CTAG_A Cuts at: 0 186 2094 Size: 186 1908 XmnI GAAnn'nnTTC Cuts at: 0 35 122 182 2094 Size: 35 87 60 1912 Fragments arranged by size: 1912 87 60 35 Enzymes that do cut: AcuI AfeI AflII AflIII AlfI AloI AlwNI ApoI AvaI AvrII BanI BanII BbsI BbvCI BciVI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI Bsu36I BtgI BtsI DraI DraIII EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PsiI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BssSI BstYI BstZ17I BtgZI ClaI CspCI DrdI EaeI EagI EcoNI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PshAI PspOMI PspXI PsrI PvuI RsrII SalI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XcmI XhoI XmaI ZraI