################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcnI BfaI BfrI BglI BlnI BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspHI BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 7 CGCG CG^CG AccIII 87 TCCGGA T^CCGGA AlwNI 617 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 643 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 910 GGACC G^G[AT]CC BalI 1591 TGGCCA TGG^CCA BanII 28 GGGCTC G[GA]GC[CT]^C BcgI 690 CGAAGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1213 GGATAC GTATCC(6/5) BclI 1269 TGATCA T^GATCA BlpI 21 GCTCAGC GC^T[ACGT]AGC Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC BsaBI 1234 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 87 TCCGGA [AT]^CCGG[AT] BseAI 87 TCCGGA T^CCGGA BseRI 808 GAGGAG GAGGAG(10/8) BsiYI 570 CCTTAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 570 CCTTAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 973 GAATGC GAATGC(1/-1) Bsp1407I 1108 TGTACA T^GTACA BspEI 87 TCCGGA T^CCGGA BsrFI 889 GCCGGT [GA]^CCGG[CT] BsrGI 1108 TGTACA T^GTACA BstBI 688 TTCGAA TT^CGAA BstUI 7 CGCG CG^CG CelII 21 GCTCAGC GC^T[ACGT]AGC Cfr10I 889 GCCGGT [GA]^CCGG[CT] EaeI 1591 TGGCCA [CT]^GGCC[GA] EcoT14I 282 CCAAGG C^C[AT][AT]GG FbaI 1269 TGATCA T^GATCA HaeII 1550 GGCGCT [GA]GCGC^[CT] HgaI 8 GCGTC GACGC(5/10) MamI 1234 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1591 TGGCCA TGG^CCA MroI 87 TCCGGA T^CCGGA MscI 1591 TGGCCA TGG^CCA MspA1I 24 CAGCGG C[AC]G^C[GT]G MvnI 7 CGCG CG^CG NspV 688 TTCGAA TT^CGAA PstI 681 CTGCAG CTGCA^G SfuI 688 TTCGAA TT^CGAA SspBI 1108 TGTACA T^GTACA SspI 989 AATATT AAT^ATT StuI 958 AGGCCT AGG^CCT StyI 282 CCAAGG C^C[AT][AT]GG TaqI 689 TCGA T^CGA TthHB8I 689 TCGA T^CGA XmnI 643 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 511 GAGCAC G[AT]GC[AT]^C 612 GTGCTC BpmI 1454 CTCCAG CTGGAG(16/14) 388 CTGGAG BsaJI 282 CCAAGG C^C[ACGT][ACGT]GG 386 CCCTGG BsiHKAI 511 GAGCAC G[AT]GC[AT]^C 612 GTGCTC BsmFI 823 GGGAC GGGAC(10/14) 723 GTCCC BssKI 387 CCTGG ^CC[ACGT]GG 1486 CCTGG BstNI 387 CCTGG CC^[AT]GG 1486 CCTGG BstXI 524 CCATAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1582 CCAATGAGATGG CfoI 6 GCGC GCG^C 1551 GCGC Cfr13I 910 GGACC G^G[ACGT]CC 1141 GGCCC DraI 493 TTTAAA TTT^AAA 883 TTTAAA DraIII 1218 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 1220 CACAGTGTG EcoRII 387 CCTGG ^CC[AT]GG 1486 CCTGG FauI 26 GCGGG CCCGC(4/6) 35 GCGGG HapII 88 CCGG C^CGG 890 CCGG HhaI 6 GCGC GCG^C 1551 GCGC HinP1I 6 GCGC G^CGC 1551 GCGC HpaII 88 CCGG C^CGG 890 CCGG MspI 88 CCGG C^CGG 890 CCGG MvaI 387 CCTGG CC^[AT]GG 1486 CCTGG Sau96I 910 GGACC G^G[ACGT]CC 1141 GGCCC ScrFI 387 CCTGG CC^[ACGT]GG 1486 CCTGG Tsp45I 815 GTGAC ^GT[GC]AC 1430 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI BbsI BbvI BglII BmyI BpuAI Bsp1286I BspLU11I BsrDI BsrI BstF5I BstYI Cac8I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII