(Circular) (Six-base) MAPSORT of: pFN21AE5676.seq Check: 2984 from: 1 to: 6442 pFN21AE5676 6442 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2385 2931 3135 4505 5889 6157 1632 Size: 753 546 204 1370 1384 268 1917 Fragments arranged by size: 1917 1384 1370 753 546 268 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2333 2416 2879 5341 6318 1203 Size: 1130 83 463 2462 977 1327 Fragments arranged by size: 2462 1327 1130 977 463 83 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 3441 1166 Size: 2275 4167 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6442 AlwNI CAG_nnn'CTG Cuts at: 2627 4185 5757 2627 Size: 1558 1572 3312 Fragments arranged by size: 3312 1572 1558 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2179 2272 3263 3751 3977 4129 6238 1493 Size: 686 93 991 488 226 152 2109 1697 Fragments arranged by size: 2109 1697 991 686 488 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3171 3204 3171 Size: 33 6409 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3763 5218 618 Size: 325 217 627 1976 1455 1842 Fragments arranged by size: 1976 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 3761 1391 Size: 646 1724 4072 Fragments arranged by size: 4072 1724 646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2391 2703 2729 928 Size: 971 492 312 26 4641 Fragments arranged by size: 4641 971 492 312 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2684 2718 4633 4667 1903 Size: 34 747 34 1915 34 3678 Fragments arranged by size: 3678 1915 747 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3212 4334 5544 3212 Size: 1122 1210 4110 Fragments arranged by size: 4110 1210 1122 BclI T'GATC_A Cuts at: 1548 3274 1548 Size: 1726 4716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5053 136 Size: 107 122 71 631 605 3381 1525 Fragments arranged by size: 3381 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2488 2673 3481 6437 2488 Size: 185 808 2956 2493 Fragments arranged by size: 2956 2493 808 185 BlpI GC'TnA_GC Cuts at: 2027 3859 2027 Size: 1832 4610 Bme1580I G_kGCm'C Cuts at: 1790 2848 4503 5659 1790 Size: 1058 1655 1156 2573 Fragments arranged by size: 2573 1655 1156 1058 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2725 3054 4173 Size: 416 53 83 399 24 1308 329 1119 Cuts at: 4173 5131 442 Size: 958 1753 Fragments arranged by size: 1753 1308 1119 958 416 399 329 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 3444 5102 1239 Size: 1175 1030 1658 2579 Fragments arranged by size: 2579 1658 1175 1030 Bpu10I CC'TnA_GC Cuts at: 2620 2620 Size: 6442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5105 882 Size: 617 3606 2219 Fragments arranged by size: 3606 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 3243 6436 3243 Size: 3193 3249 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4249 4631 5266 275 Size: 53 83 186 936 2716 382 635 1451 Fragments arranged by size: 2716 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2092 4875 5273 5547 5694 6105 2092 Size: 2783 398 274 147 411 2429 Fragments arranged by size: 2783 2429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6217 Fragments arranged by size: 6217 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 1186 Size: 1642 4800 BseYI C'CCAG_C Cuts at: 5645 6141 5645 Size: 496 5946 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5869 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3892 4653 5681 1540 Size: 275 186 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 2621 4503 4588 5659 2520 Size: 101 1882 85 1071 3303 Fragments arranged by size: 3303 1882 1071 101 85 BsmI GAATG_Cn' Cuts at: 2984 3953 4046 2984 Size: 969 93 5380 Fragments arranged by size: 5380 969 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2520 2621 2848 3761 4503 4588 Size: 399 247 483 101 227 913 742 85 Cuts at: 4588 5659 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 913 742 483 399 247 227 101 85 BspEI T'CCGG_A Cuts at: 2092 6105 2092 Size: 4013 2429 BspHI T'CATG_A Cuts at: 4331 6061 4331 Size: 1730 4712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 3252 3406 4937 5111 65 Size: 1183 1759 245 154 1531 174 1396 Fragments arranged by size: 1759 1531 1396 1183 245 174 154 BsrFI r'CCGG_y Cuts at: 1700 1810 2894 5086 5273 1700 Size: 110 1084 2192 187 2869 Fragments arranged by size: 2869 2192 1084 187 110 BsrGI T'GTAC_A Cuts at: 96 3113 96 Size: 3017 3425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 2694 5246 2694 Size: 2552 3890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstXI CCAn_nnnn'nTGG Cuts at: 2536 3594 2536 Size: 1058 5384 BstYI r'GATC_y Cuts at: 1972 2488 2673 2758 3072 3481 3772 4139 4524 Size: 516 185 85 314 409 291 367 385 Cuts at: 4524 4541 5993 6079 6091 6437 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 409 385 367 346 314 291 185 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5890 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3720 487 Size: 683 56 2494 3209 Fragments arranged by size: 3209 2494 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3958 4752 4772 2787 Size: 1171 794 20 4457 Fragments arranged by size: 4457 1171 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2298 2333 3278 3313 529 Size: 35 1734 35 945 35 3658 Fragments arranged by size: 3658 1734 945 35 35 35 DraI TTT'AAA Cuts at: 2500 2890 3746 2500 Size: 390 856 5196 Fragments arranged by size: 5196 856 390 DraIII CAC_nnn'GTG Cuts at: 3228 3230 3228 Size: 2 6440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3596 3889 4778 Size: 54 1425 50 275 18 1766 293 889 Cuts at: 4778 6407 8 Size: 1629 43 Fragments arranged by size: 1766 1629 1425 889 293 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4372 1353 Size: 3019 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2385 2414 2931 3135 3444 4505 5102 Size: 393 753 29 517 204 309 1061 597 Cuts at: 5102 5889 6157 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1061 787 753 597 517 393 309 268 204 29 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 3559 5589 1678 Size: 1881 2030 2531 Fragments arranged by size: 2531 2030 1881 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2465 2497 3034 3066 5083 Size: 32 135 32 893 32 537 32 2017 Cuts at: 5083 5115 5157 5189 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2017 893 537 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3786 4032 669 Size: 988 281 1848 246 3079 Fragments arranged by size: 3079 1848 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5556 5740 1305 Size: 4251 184 2007 Fragments arranged by size: 4251 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3598 10 Size: 54 1425 343 1766 2854 Fragments arranged by size: 2854 1766 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2661 3054 3257 3441 4400 4759 4918 Size: 648 1495 393 203 184 959 359 159 Cuts at: 4918 518 Size: 2042 Fragments arranged by size: 2042 1495 959 648 393 359 203 184 159 MspA1I CmG'CkG Cuts at: 1606 2031 3858 4535 5683 5928 1606 Size: 425 1827 677 1148 245 2120 Fragments arranged by size: 2120 1827 1148 677 425 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 2337 2420 2865 2883 3800 5345 1132 Size: 97 1108 83 445 18 917 1545 2229 Fragments arranged by size: 2229 1545 1108 917 445 97 83 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 2333 2416 2879 5341 2333 Size: 83 463 2462 3434 Fragments arranged by size: 3434 2462 463 83 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PstI C_TGCA'G Cuts at: 830 2690 3794 830 Size: 1860 1104 3478 Fragments arranged by size: 3478 1860 1104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 2357 2686 3439 3525 3790 4925 5606 Size: 219 1312 329 753 86 265 1135 681 Cuts at: 5606 5797 826 Size: 191 1471 Fragments arranged by size: 1471 1312 1135 753 681 329 265 219 191 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 5447 5709 820 Size: 197 926 2603 901 262 1553 Fragments arranged by size: 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3800 1132 Size: 2668 3774 SspI AAT'ATT Cuts at: 5 52 2996 4366 5 Size: 47 2944 1370 2081 Fragments arranged by size: 2944 2081 1370 47 StuI AGG'CCT Cuts at: 1517 2965 1517 Size: 1448 4994 StyI C'CwwG_G Cuts at: 513 1065 2287 3881 513 Size: 552 1222 1594 3074 Fragments arranged by size: 3074 1594 1222 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4469 4486 4639 4824 5269 325 Size: 1255 2889 17 153 185 445 1498 Fragments arranged by size: 2889 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2336 3113 3383 Size: 274 80 33 51 1443 359 777 270 Cuts at: 3383 3637 4688 6400 96 Size: 254 1051 1712 138 Fragments arranged by size: 1712 1443 1051 777 359 274 270 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6402 6434 6402 Size: 32 6410 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 2652 4571 2652 Size: 1919 4523 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI