(Linear) (Six-base) MAPSORT of: KIEE5676.seq Check: 582 from: 1 to: 1737 KIEE5676.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 926 1130 1737 Size: 380 546 204 607 Fragments arranged by size: 607 546 380 204 AflIII A'CryG_T Cuts at: 0 328 411 874 1737 Size: 328 83 463 863 Fragments arranged by size: 863 463 328 83 AleI CACnn'nnGTG Cuts at: 0 1436 1737 Size: 1436 301 AlwNI CAG_nnn'CTG Cuts at: 0 622 1737 Size: 622 1115 ApoI r'AATT_y Cuts at: 0 174 267 1258 1737 Size: 174 93 991 479 Fragments arranged by size: 991 479 174 93 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1166 1199 1737 Size: 1166 33 538 Fragments arranged by size: 1166 538 33 BanII G_rGCy'C Cuts at: 0 32 1737 Size: 32 1705 BbsI GAAGACnn'nnnn_ Cuts at: 0 386 698 724 1737 Size: 386 312 26 1013 Fragments arranged by size: 1013 386 312 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 679 713 1737 Size: 679 34 1024 Fragments arranged by size: 1024 679 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1207 1737 Size: 1207 530 BclI T'GATC_A Cuts at: 0 1269 1737 Size: 1269 468 BglII A'GATC_T Cuts at: 0 483 668 1476 1737 Size: 483 185 808 261 Fragments arranged by size: 808 483 261 185 BlpI GC'TnA_GC Cuts at: 0 22 1737 Size: 22 1715 Bme1580I G_kGCm'C Cuts at: 0 843 1737 Size: 843 894 BmrI ACTGGGnnnn_n' Cuts at: 0 720 1049 1737 Size: 720 329 688 Fragments arranged by size: 720 688 329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 1439 1737 Size: 409 1030 298 Fragments arranged by size: 1030 409 298 Bpu10I CC'TnA_GC Cuts at: 0 615 1737 Size: 615 1122 BsaBI GATnn'nnATC Cuts at: 0 1238 1737 Size: 1238 499 BsaWI w'CCGG_w Cuts at: 0 87 1737 Size: 87 1650 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 823 1737 Size: 823 914 BsiHKAI G_wGCw'C Cuts at: 0 515 616 1737 Size: 515 101 1121 Fragments arranged by size: 1121 515 101 BsmI GAATG_Cn' Cuts at: 0 979 1737 Size: 979 758 Bsp1286I G_dGCh'C Cuts at: 0 32 515 616 843 1737 Size: 32 483 101 227 894 Fragments arranged by size: 894 483 227 101 32 BspEI T'CCGG_A Cuts at: 0 87 1737 Size: 87 1650 BsrDI GCAATG_nn' Cuts at: 0 1002 1247 1401 1737 Size: 1002 245 154 336 Fragments arranged by size: 1002 336 245 154 BsrFI r'CCGG_y Cuts at: 0 889 1737 Size: 889 848 BsrGI T'GTAC_A Cuts at: 0 1108 1737 Size: 1108 629 BstBI TT'CG_AA Cuts at: 0 689 1737 Size: 689 1048 BstXI CCAn_nnnn'nTGG Cuts at: 0 531 1589 1737 Size: 531 1058 148 Fragments arranged by size: 1058 531 148 BstYI r'GATC_y Cuts at: 0 483 668 753 1067 1476 1737 Size: 483 185 85 314 409 261 Fragments arranged by size: 483 409 314 261 185 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1715 1737 Size: 1715 22 BtsI GCAGTG_nn' Cuts at: 0 782 1737 Size: 782 955 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 293 328 1273 1308 1737 Size: 293 35 945 35 429 Fragments arranged by size: 945 429 293 35 35 DraI TTT'AAA Cuts at: 0 495 885 1737 Size: 495 390 852 Fragments arranged by size: 852 495 390 DraIII CAC_nnn'GTG Cuts at: 0 1223 1225 1737 Size: 1223 2 512 Fragments arranged by size: 1223 512 2 EaeI y'GGCC_r Cuts at: 0 1591 1737 Size: 1591 146 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 409 926 1130 1439 1737 Size: 380 29 517 204 309 298 Fragments arranged by size: 517 380 309 298 204 29 HaeII r_GCGC'y Cuts at: 0 1554 1737 Size: 1554 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 460 492 1029 1061 1737 Size: 460 32 537 32 676 Fragments arranged by size: 676 537 460 32 32 MscI TGG'CCA Cuts at: 0 1593 1737 Size: 1593 144 MslI CAynn'nnrTG Cuts at: 0 656 1049 1252 1436 1737 Size: 656 393 203 184 301 Fragments arranged by size: 656 393 301 203 184 MspA1I CmG'CkG Cuts at: 0 26 1737 Size: 26 1711 NspI r_CATG'y Cuts at: 0 332 415 860 878 1737 Size: 332 83 445 18 859 Fragments arranged by size: 859 445 332 83 18 PciI A'CATG_T Cuts at: 0 328 411 874 1737 Size: 328 83 463 863 Fragments arranged by size: 863 463 328 83 PstI C_TGCA'G Cuts at: 0 685 1737 Size: 685 1052 SfcI C'TryA_G Cuts at: 0 352 681 1434 1520 1737 Size: 352 329 753 86 217 Fragments arranged by size: 753 352 329 217 86 SspI AAT'ATT Cuts at: 0 991 1737 Size: 991 746 StuI AGG'CCT Cuts at: 0 960 1737 Size: 960 777 StyI C'CwwG_G Cuts at: 0 282 1737 Size: 282 1455 TatI w'GTAC_w Cuts at: 0 331 1108 1378 1632 1737 Size: 331 777 270 254 105 Fragments arranged by size: 777 331 270 254 105 XmnI GAAnn'nnTTC Cuts at: 0 647 1737 Size: 647 1090 Enzymes that do cut: AcuI AflIII AleI AlwNI ApoI BaeI BanII BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgZI BtsI CspCI DraI DraIII EaeI Eco57MI HaeII Hin4I MscI MslI MspA1I NspI PciI PstI SfcI SspI StuI StyI TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BbvCI BfrBI BglI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI ClaI DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI