################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BmyI BpmI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FokI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 357 GAATTT [GA]^AATT[CT] AfaI 443 GTAC GT^AC AhdI 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 349 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 357 GAATTT [GA]^AATT[CT] AspEI 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 209 GTCTTC GAAGAC(2/6) BclI 387 TGATCA T^GATCA BlpI 402 GCTGAGC GC^T[ACGT]AGC Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC BpuAI 209 GTCTTC GAAGAC(2/6) BsaBI 319 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC CelII 402 GCTGAGC GC^T[ACGT]AGC CfoI 108 GCGC GCG^C Cfr13I 379 GGCCC G^G[ACGT]CC DsaI 424 CCATGG C^C[GA][CT]GG Eam1105I 288 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 424 CCATGG C^C[AT][AT]GG EcoT22I 1 ATGCAT ATGCA^T FbaI 387 TGATCA T^GATCA HaeII 107 GGCGCT [GA]GCGC^[CT] HhaI 108 GCGC GCG^C HinP1I 108 GCGC G^CGC HindIII 342 AAGCTT A^AGCTT HinfI 310 GACTC G^A[ACGT]TC MamI 319 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 210 TCTTC GAAGA(8/7) MnlI 300 GAGG CCTC(7/6) NcoI 424 CCATGG C^CATGG NsiI 1 ATGCAT ATGCA^T NspI 220 GCATGC [GA]CATG^[CT] PflMI 278 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 310 GACTC GAGTC(4/5) RsaI 443 GTAC GT^AC Sau96I 379 GGCCC G^G[ACGT]CC SfaNI 319 GATGC GCATC(5/9) SphI 220 GCATGC GCATG^C StyI 424 CCATGG C^C[AT][AT]GG Tsp45I 308 GTGAC ^GT[GC]AC TspRI 391 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 278 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 382 CCATCTGATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BbvI 160 GCTGC GCAGC(8/12) 217 GCTGC BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG 424 CCATGG BsiYI 98 CCTTAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 278 CCAAGAACTGG BslI 98 CCTTAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 278 CCAAGAACTGG BsmI 69 GCATTC GAATGC(1/-1) 81 GCATTC BsrI 284 ACTGG ACTGG(1/-1) 392 ACTGG BssKI 58 CCAGG ^CC[ACGT]GG 348 CCAGG BstNI 58 CCAGG CC^[AT]GG 348 CCAGG BstXI 226 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 424 CCATGGAAATGG Cac8I 48 GCCTGC GC[ACGT]^[ACGT]GC 220 GCATGC DdeI 332 CTCAG C^T[ACGT]AG 403 CTGAG DpnII 325 GATC ^GATC 388 GATC EcoRII 58 CCAGG ^CC[AT]GG 348 CCAGG Fnu4HI 160 GCTGC GC^[ACGT]GC 217 GCTGC HaeIII 379 GGCC GG^CC 418 GGCC ItaI 160 GCTGC GC^[ACGT]GC 217 GCTGC MaeIII 11 GTAAC ^GT[ACGT]AC 308 GTGAC MboI 325 GATC ^GATC 388 GATC MslI 200 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 425 CATGGAAATG MvaI 58 CCAGG CC^[AT]GG 348 CCAGG NdeII 325 GATC ^GATC 388 GATC Sau3AI 325 GATC ^GATC 388 GATC ScrFI 58 CCAGG CC^[ACGT]GG 348 CCAGG TseI 160 GCTGC G^C[AT]GC 217 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AluI MseI MwoI NlaIII Tru9I Tsp509I