(Linear) (Six-base) MAPSORT of: KIEE6556.seq  Check: 6192  from: 1  to: 462



KIEE6556.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     293     462 
   Size:        293     169
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     344     376     462 
   Size:        344      32      86

  Fragments arranged by size: 

                 344      86      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     354     462 
   Size:        354     108
 
ApoI r'AATT_y

Cuts at:      0     357     462 
   Size:        357     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     202     462 
   Size:        202     260
 
BclI T'GATC_A

Cuts at:      0     387     462 
   Size:        387      75
 
BfrBI ATG'CAT

Cuts at:      0       3     462 
   Size:          3     459
 
BlpI GC'TnA_GC

Cuts at:      0     403     462 
   Size:        403      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     332     462 
   Size:        332     130
 
BsaBI GATnn'nnATC

Cuts at:      0     323     462 
   Size:        323     139
 
BsmI GAATG_Cn'

Cuts at:      0      69      81     462 
   Size:         69      12     381

  Fragments arranged by size: 

                 381      69      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     233     431     462 
   Size:        233     198      31

  Fragments arranged by size: 

                 233     198      31
 
BtgI C'CryG_G

Cuts at:      0     424     462 
   Size:        424      38
 
HaeII r_GCGC'y

Cuts at:      0     111     462 
   Size:        111     351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     279     311     462 
   Size:        279      32     151

  Fragments arranged by size: 

                 279     151      32
 
HindIII A'AGCT_T

Cuts at:      0     342     462 
   Size:        342     120
 
MslI CAynn'nnrTG

Cuts at:      0     204     429     462 
   Size:        204     225      33

  Fragments arranged by size: 

                 225     204      33
 
NcoI C'CATG_G

Cuts at:      0     424     462 
   Size:        424      38
 
NsiI A_TGCA'T

Cuts at:      0       5     462 
   Size:          5     457
 
NspI r_CATG'y

Cuts at:      0     224     462 
   Size:        224     238
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     284     462 
   Size:        284     178
 
PfoI T'CCnGG_A

Cuts at:      0     347     462 
   Size:        347     115
 
SphI G_CATG'C

Cuts at:      0     224     462 
   Size:        224     238
 
StyI C'CwwG_G

Cuts at:      0     424     462 
   Size:        424      38
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     389     462 
   Size:        389      73
 

 Enzymes that do cut:

     AhdI     AloI    AlwNI     ApoI     BbsI     BclI    BfrBI     BlpI
   Bpu10I    BsaBI     BsmI    BstXI     BtgI    HaeII    Hin4I  HindIII
     MslI     NcoI     NsiI     NspI    PflMI     PfoI     SphI     StyI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI