################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 832 AAATTT [GA]^AATT[CT] AfaI 553 GTAC GT^AC ApoI 832 AAATTT [GA]^AATT[CT] BalI 492 TGGCCA TGG^CCA BanI 682 GGTGCC G^G[CT][GA]CC BciVI 804 GGATAC GTATCC(6/5) BfaI 928 CTAG C^TAG BsaAI 631 TACGTG [CT]AC^GT[GA] BsgI 711 CTGCAC GTGCAG(16/14) BsiEI 66 CGGCCG CG[GA][CT]^CG BsmFI 202 GGGAC GGGAC(10/14) BsmI 777 GAATGC GAATGC(1/-1) BspMI 122 GCAGGT ACCTGC(4/8) BstF5I 826 GGATG GGATG(2/0) BstYI 719 AGATCC [GA]^GATC[CT] CfoI 19 GCGC GCG^C DraIII 668 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 800 CCATGG C^C[GA][CT]GG EagI 66 CGGCCG C^GGCCG EclXI 66 CGGCCG C^GGCCG Eco52I 66 CGGCCG C^GGCCG EcoT14I 800 CCATGG C^C[AT][AT]GG FauI 102 CCCGC CCCGC(4/6) FokI 826 GGATG GGATG(9/13) HhaI 19 GCGC GCG^C HinP1I 19 GCGC G^CGC HphI 211 GGTGA GGTGA(8/7) MaeI 928 CTAG C^TAG MflI 719 AGATCC [GA]^GATC[CT] MluNI 492 TGGCCA TGG^CCA MscI 492 TGGCCA TGG^CCA NcoI 800 CCATGG C^CATGG NotI 65 GCGGCCGC GC^GGCCGC PflMI 664 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 288 GAGTC GAGTC(4/5) PstI 562 CTGCAG CTGCA^G RsaI 553 GTAC GT^AC SfaNI 676 GCATC GCATC(5/9) SfcI 562 CTGCAG C^T[GA][CT]AG SmlI 603 CTCAAG C^T[CT][GA]AG StyI 800 CCATGG C^C[AT][AT]GG Tsp45I 505 GTGAC ^GT[GC]AC Van91I 664 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 791 CCATTATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 719 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspHI 82 GAGCTC G[AT]GC[AT]^C 863 GAGCTC BanII 82 GAGCTC G[GA]GC[CT]^C 863 GAGCTC BclI 575 TGATCA T^GATCA 840 TGATCA BmyI 82 GAGCTC G[AGT]GC[ACT]^C 863 GAGCTC BseRI 112 CTCCTC GAGGAG(10/8) 79 GAGGAG BsiHKAI 82 GAGCTC G[AT]GC[AT]^C 863 GAGCTC BsmAI 446 GAGAC GTCTC(1/5) 290 GTCTC Bsp1286I 82 GAGCTC G[AGT]GC[ACT]^C 863 GAGCTC BspHI 689 TCATGA T^CATGA 897 TCATGA BstAPI 676 GCATCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 772 GCAGGGAATGC Cac8I 494 GCCAGC GC[ACGT]^[ACGT]GC 735 GCTTGC FbaI 575 TGATCA T^GATCA 840 TGATCA HgaI 119 GACGC GACGC(5/10) 55 GCGTC MaeII 632 ACGT A^CGT 763 ACGT MseI 128 TTAA T^TAA 464 TTAA RcaI 689 TCATGA T^CATGA 897 TCATGA SacI 82 GAGCTC GAGCT^C 863 GAGCTC TaqI 186 TCGA T^CGA 592 TCGA Tru9I 128 TTAA T^TAA 464 TTAA Tsp509I 619 AATT ^AATT 833 AATT TspRI 87 CACTG CA[GC]TG[ACGT][ACGT]^ 368 CACTG TthHB8I 186 TCGA T^CGA 592 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BsaJI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI TfiI TseI