(Circular) (Six-base) MAPSORT of: pFN21AE6580.seq Check: 5028 from: 1 to: 5098 pFN21AE6580 5098 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2245 2245 Size: 5098 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2908 278 Size: 53 83 186 936 1372 2468 Fragments arranged by size: 2468 1372 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2441 1937 Size: 504 4594 Acc65I G'GTAC_C Cuts at: 2419 2419 Size: 5098 AclI AA'CG_TT Cuts at: 1563 3225 3598 1563 Size: 1662 373 3063 Fragments arranged by size: 3063 1662 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 3161 4545 4813 1632 Size: 608 921 1384 268 1917 Fragments arranged by size: 1917 1384 921 608 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4901 AflIII A'CryG_T Cuts at: 1203 3997 4974 1203 Size: 2794 977 1327 Fragments arranged by size: 2794 1327 977 AgeI A'CCGG_T Cuts at: 3929 3929 Size: 5098 AhdI GACnn_n'nnGTC Cuts at: 3827 3827 Size: 5098 AleI CACnn'nnGTG Cuts at: 1166 2039 2195 1166 Size: 873 156 4069 Fragments arranged by size: 4069 873 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5098 AlwNI CAG_nnn'CTG Cuts at: 2134 2841 4413 2134 Size: 707 1572 2819 Fragments arranged by size: 2819 1572 707 ApaLI G'TGCA_C Cuts at: 3155 4311 3155 Size: 1156 3942 ApoI r'AATT_y Cuts at: 1493 2407 2633 2785 4894 1493 Size: 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 226 152 AseI AT'TA_AT Cuts at: 160 1032 3652 160 Size: 872 2620 1606 Fragments arranged by size: 2620 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5098 AvaI C'yCGr_G Cuts at: 1870 1943 2423 1870 Size: 73 480 4545 Fragments arranged by size: 4545 480 73 BamHI G'GATC_C Cuts at: 2428 2795 2428 Size: 367 4731 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2419 3874 618 Size: 325 217 627 632 1455 1842 Fragments arranged by size: 1842 1455 632 627 325 217 BanII G_rGCy'C Cuts at: 1391 2417 1391 Size: 1026 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 928 Size: 971 307 3820 Fragments arranged by size: 3820 971 307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3289 3323 1903 Size: 34 1352 34 3678 Fragments arranged by size: 3678 1352 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2990 4200 2990 Size: 1210 3888 BclI T'GATC_A Cuts at: 1548 1548 Size: 5098 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3709 136 Size: 107 122 71 631 605 2037 1525 Fragments arranged by size: 2037 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5093 5093 Size: 5098 BlpI GC'TnA_GC Cuts at: 2048 2515 2048 Size: 467 4631 Bme1580I G_kGCm'C Cuts at: 1790 3159 4315 1790 Size: 1369 1156 2573 Fragments arranged by size: 2573 1369 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2829 3787 442 Size: 416 53 83 399 24 1412 958 1753 Fragments arranged by size: 1753 1412 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5098 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 5098 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3758 1239 Size: 2519 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3223 4088 4386 3223 Size: 865 298 3935 Fragments arranged by size: 3935 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2174 3761 882 Size: 617 675 1587 2219 Fragments arranged by size: 2219 1587 675 617 BsaAI yAC'GTr Cuts at: 493 1204 2889 493 Size: 711 1685 2702 Fragments arranged by size: 2702 1685 711 BsaBI GATnn'nnATC Cuts at: 5092 5092 Size: 5098 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2905 3287 3922 275 Size: 53 83 186 936 1372 382 635 1451 Fragments arranged by size: 1451 1372 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3531 3929 4203 4350 4761 3531 Size: 398 274 147 411 3868 Fragments arranged by size: 3868 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4873 Fragments arranged by size: 4873 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2279 2384 1186 Size: 1006 87 105 3900 Fragments arranged by size: 3900 1006 105 87 BseYI C'CCAG_C Cuts at: 2022 2146 4301 4797 2022 Size: 124 2155 496 2323 Fragments arranged by size: 2323 2155 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2548 3309 4337 1540 Size: 275 186 547 761 1028 2301 Fragments arranged by size: 2301 1028 761 547 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 3159 3244 4315 2367 Size: 792 85 1071 3150 Fragments arranged by size: 3150 1071 792 85 BsmI GAATG_Cn' Cuts at: 2609 2702 2609 Size: 93 5005 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2417 3159 3244 4315 1391 Size: 399 577 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 399 85 50 BspEI T'CCGG_A Cuts at: 4761 4761 Size: 5098 BspHI T'CATG_A Cuts at: 2987 4717 2987 Size: 1730 3368 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2245 2453 844 Size: 1401 208 3489 Fragments arranged by size: 3489 1401 208 BsrBI CCG'CTC Cuts at: 1394 1823 2985 1394 Size: 429 1162 3507 Fragments arranged by size: 3507 1162 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3593 3767 65 Size: 1183 2345 174 1396 Fragments arranged by size: 2345 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3742 3929 1700 Size: 110 1932 187 2869 Fragments arranged by size: 2869 1932 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5098 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5098 BssSI C'ACGA_G Cuts at: 2366 3158 4170 2366 Size: 792 1012 3294 Fragments arranged by size: 3294 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5098 BstBI TT'CG_AA Cuts at: 3902 3902 Size: 5098 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5098 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5098 BstYI r'GATC_y Cuts at: 1972 2428 2795 3180 3197 4649 4735 4747 5093 Size: 456 367 385 17 1452 86 12 346 Cuts at: 5093 1972 Size: 1977 Fragments arranged by size: 1977 1452 456 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4546 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4359 Fragments arranged by size: 4359 683 56 BtsI GCAGTG_nn' Cuts at: 2614 3408 3428 2614 Size: 794 20 4284 Fragments arranged by size: 4284 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5098 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5063 DraI TTT'AAA Cuts at: 2402 2402 Size: 5098 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4105 809 Size: 507 2789 1802 Fragments arranged by size: 2789 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2545 3434 5063 Size: 54 1425 50 275 18 715 889 1629 Cuts at: 5063 8 Size: 43 Fragments arranged by size: 1629 1425 889 715 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2545 1537 Size: 275 733 4090 Fragments arranged by size: 4090 733 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3028 1353 Size: 1675 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3685 4059 4205 387 Size: 1064 138 2096 374 146 1280 Fragments arranged by size: 2096 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 3161 3758 4545 4813 1239 Size: 393 608 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 608 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2097 2161 2097 Size: 64 5034 EcoRI G'AATT_C Cuts at: 2407 2785 2407 Size: 378 4720 FspI TGC'GCA Cuts at: 3604 3604 Size: 5098 HaeII r_GCGC'y Cuts at: 1678 2349 4245 1678 Size: 671 1896 2531 Fragments arranged by size: 2531 1896 671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 3739 3771 3813 Size: 32 135 32 794 32 1341 32 42 Cuts at: 3813 3845 1373 Size: 32 2626 Fragments arranged by size: 2626 1341 794 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2442 2688 669 Size: 988 281 504 246 3079 Fragments arranged by size: 3079 988 504 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5098 HpaI GTT'AAC Cuts at: 2688 2688 Size: 5098 KpnI G_GTAC'C Cuts at: 2423 2423 Size: 5098 MfeI C'AATT_G Cuts at: 2697 2697 Size: 5098 MluI A'CGCG_T Cuts at: 4974 4974 Size: 5098 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4212 4396 1305 Size: 2907 184 2007 Fragments arranged by size: 2907 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3276 Fragments arranged by size: 3276 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2195 3056 3415 3574 518 Size: 648 873 156 861 359 159 2042 Fragments arranged by size: 2042 873 861 648 359 159 156 MspA1I CmG'CkG Cuts at: 1606 2026 2067 2514 3191 4339 4584 1606 Size: 420 41 447 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 447 420 245 41 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4546 NdeI CA'TA_TG Cuts at: 387 387 Size: 5098 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5098 NotI GC'GGCC_GC Cuts at: 2545 2545 Size: 5098 NspI r_CATG'y Cuts at: 1132 1229 2456 4001 1132 Size: 97 1227 1545 2229 Fragments arranged by size: 2229 1545 1227 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5098 PciI A'CATG_T Cuts at: 3997 3997 Size: 5098 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 2134 1108 Size: 921 105 4072 Fragments arranged by size: 4072 921 105 PmeI GTTT'AAAC Cuts at: 2402 2402 Size: 5098 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3167 3199 4680 4712 3167 Size: 32 1481 32 3553 Fragments arranged by size: 3553 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 2097 Size: 5098 PshAI GACnn'nnGTC Cuts at: 1613 2015 1613 Size: 402 4696 PsiI TTA'TAA Cuts at: 2668 2668 Size: 5098 PstI C_TGCA'G Cuts at: 830 2450 830 Size: 1620 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5098 PvuII CAG'CTG Cuts at: 2026 2067 2026 Size: 41 5057 SalI G'TCGA_C Cuts at: 1936 2440 1936 Size: 504 4594 SanDI GG'GwC_CC Cuts at: 2097 2097 Size: 5098 SbfI CC_TGCA'GG Cuts at: 2450 2450 Size: 5098 ScaI AGT'ACT Cuts at: 3346 5058 3346 Size: 1712 3386 SfcI C'TryA_G Cuts at: 826 1045 2446 3581 4262 4453 826 Size: 219 1401 1135 681 191 1471 Fragments arranged by size: 1471 1401 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5098 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5098 SmaI CCC'GGG Cuts at: 1872 2425 1872 Size: 553 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3202 4103 4365 820 Size: 197 926 1259 901 262 1553 Fragments arranged by size: 1553 1259 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2889 493 Size: 2396 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5098 SphI G_CATG'C Cuts at: 1132 2456 1132 Size: 1324 3774 SspI AAT'ATT Cuts at: 5 52 2242 3022 5 Size: 47 2190 780 2081 Fragments arranged by size: 2190 2081 780 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5098 StyI C'CwwG_G Cuts at: 513 1065 2537 513 Size: 552 1472 3074 Fragments arranged by size: 3074 1472 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 2404 3125 3142 3295 3480 3925 Size: 1255 456 368 721 17 153 185 445 Cuts at: 3925 325 Size: 1498 Fragments arranged by size: 1498 1255 721 456 445 368 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3344 5056 96 Size: 274 80 33 51 1443 1367 1712 138 Fragments arranged by size: 1712 1443 1367 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5058 5090 5058 Size: 32 5066 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5098 XbaI T'CTAG_A Cuts at: 2434 2434 Size: 5098 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5098 XmaI C'CCGG_G Cuts at: 1870 2423 1870 Size: 553 4545 XmnI GAAnn'nnTTC Cuts at: 3227 3227 Size: 5098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2906 276 Size: 53 83 186 936 1372 2468 Fragments arranged by size: 2468 1372 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI