(Linear) (Six-base) MAPSORT of: KIEE6580.seq  Check: 164  from: 1  to: 393



KIEE6580.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     240     393 
   Size:        240     153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     393 
   Size:        235     158
 
AleI CACnn'nnGTG

Cuts at:      0      34     190     393 
   Size:         34     156     203

  Fragments arranged by size: 

                 203     156      34
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     393 
   Size:        129     264
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201     393 
   Size:        201     192
 
BlpI GC'TnA_GC

Cuts at:      0      43     393 
   Size:         43     350
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     198     393 
   Size:        198     195
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169     393 
   Size:        169     224
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187     274     379     393 
   Size:        187      87     105      14

  Fragments arranged by size: 

                 187     105      87      14
 
BseYI C'CCAG_C

Cuts at:      0      17     141     393 
   Size:         17     124     252

  Fragments arranged by size: 

                 252     124      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     393 
   Size:        362      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     362     393 
   Size:        362      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     240     393 
   Size:        240     153
 
BssSI C'ACGA_G

Cuts at:      0     361     393 
   Size:        361      32
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     192     393 
   Size:        192     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     393 
   Size:        235     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92     156     393 
   Size:         92      64     237

  Fragments arranged by size: 

                 237      92      64
 
HaeII r_GCGC'y

Cuts at:      0     344     393 
   Size:        344      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     361     393 
   Size:        361      32
 
MslI CAynn'nnrTG

Cuts at:      0      34     190     393 
   Size:         34     156     203

  Fragments arranged by size: 

                 203     156      34
 
MspA1I CmG'CkG

Cuts at:      0      21      62     393 
   Size:         21      41     331

  Fragments arranged by size: 

                 331      41      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      24     129     393 
   Size:         24     105     264

  Fragments arranged by size: 

                 264     105      24
 
PpuMI rG'GwC_Cy

Cuts at:      0      92     393 
   Size:         92     301
 
PshAI GACnn'nnGTC

Cuts at:      0      10     393 
   Size:         10     383
 
PvuII CAG'CTG

Cuts at:      0      21      62     393 
   Size:         21      41     331

  Fragments arranged by size: 

                 331      41      21
 
SanDI GG'GwC_CC

Cuts at:      0      92     393 
   Size:         92     301
 
SspI AAT'ATT

Cuts at:      0     237     393 
   Size:        237     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      31     393 
   Size:         31     362
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     BbsI     BlpI     BplI     BsaI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BssSI    BstXI  Eco57MI
 EcoO109I    HaeII    Hin4I     MslI   MspA1I    PflMI    PpuMI    PshAI
    PvuII    SanDI     SspI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI