Restriction map for
pFN21AE5187
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MaeIII
MamI MfeI MflI MluI MroI
MseI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TseI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 178 GAATTC [GA]^AATT[CT]
Alw44I 363 GTGCAC G^TGCAC
AlwI 245 GGATC GGATC(4/5)
ApaLI 363 GTGCAC G^TGCAC
ApoI 178 GAATTC [GA]^AATT[CT]
AspHI 363 GTGCAC G[AT]GC[AT]^C
BalI 27 TGGCCA TGG^CCA
BanI 235 GGTGCC G^G[CT][GA]CC
BbsI 299 GTCTTC GAAGAC(2/6)
BmyI 363 GTGCAC G[AGT]GC[ACT]^C
BpuAI 299 GTCTTC GAAGAC(2/6)
BsiHKAI 363 GTGCAC G[AT]GC[AT]^C
BsmI 223 GCATTC GAATGC(1/-1)
Bsp1286I 363 GTGCAC G[AGT]GC[ACT]^C
DpnII 246 GATC ^GATC
DsaI 209 CCATGG C^C[GA][CT]GG
EarI 85 GAAGAG CTCTTC(1/4)
EcoRI 178 GAATTC G^AATTC
EcoT14I 209 CCATGG C^C[AT][AT]GG
Fnu4HI 263 GCGGC GC^[ACGT]GC
ItaI 263 GCGGC GC^[ACGT]GC
Ksp632I 85 GAAGAG CTCTTC(1/4)
MboI 246 GATC ^GATC
MluNI 27 TGGCCA TGG^CCA
MscI 27 TGGCCA TGG^CCA
MslI 307 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 209 CCATGG C^CATGG
NdeII 246 GATC ^GATC
NlaIV 235 GGTGCC GG[ACGT]^[ACGT]CC
PstI 286 CTGCAG CTGCA^G
Sau3AI 246 GATC ^GATC
SexAI 294 ACCTGGT A^CC[AT]GGT
SfcI 286 CTGCAG C^T[GA][CT]AG
StyI 209 CCATGG C^C[AT][AT]GG
TfiI 160 GATTC G^A[AT]TC
Tsp509I 179 AATT ^AATT
TspRI 404 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 200 CCAATAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 191 GCGG CCGC(-3/-1)
263 GCGG
AluI 148 AGCT AG^CT
252 AGCT
BfaI 242 CTAG C^TAG
328 CTAG
BsaJI 183 CCAGGG C^C[ACGT][ACGT]GG
209 CCATGG
BsmAI 106 GAGAC GTCTC(1/5)
270 GTCTC
BssKI 183 CCAGG ^CC[ACGT]GG
295 CCTGG
BstNI 183 CCAGG CC^[AT]GG
295 CCTGG
DdeI 110 CTCAG C^T[ACGT]AG
116 CTGAG
EaeI 27 TGGCCA [CT]^GGCC[GA]
264 CGGCCA
EcoRII 183 CCAGG ^CC[AT]GG
295 CCTGG
HaeIII 28 GGCC GG^CC
265 GGCC
HphI 213 GGTGA GGTGA(8/7)
35 TCACC
MaeI 242 CTAG C^TAG
328 CTAG
MvaI 183 CCAGG CC^[AT]GG
295 CCTGG
PleI 7 GACTC GAGTC(4/5)
108 GACTC
ScrFI 183 CCAGG CC^[ACGT]GG
295 CCTGG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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BsrI HinfI MboII MnlI NlaIII