(Linear) (Six-base) MAPSORT of: KIEE5187.seq  Check: 6693  from: 1  to: 408



KIEE5187.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

ApaLI G'TGCA_C

Cuts at:      0     363     408 
   Size:        363      45
 
ApoI r'AATT_y

Cuts at:      0     178     408 
   Size:        178     230
 
BanI G'GyrC_C

Cuts at:      0     235     408 
   Size:        235     173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     292     408 
   Size:        292     116
 
Bme1580I G_kGCm'C

Cuts at:      0     367     408 
   Size:        367      41
 
BseYI C'CCAG_C

Cuts at:      0      73     274     408 
   Size:         73     201     134

  Fragments arranged by size: 

                 201     134      73
 
BsiHKAI G_wGCw'C

Cuts at:      0     367     408 
   Size:        367      41
 
BsmI GAATG_Cn'

Cuts at:      0     223     408 
   Size:        223     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     367     408 
   Size:        367      41
 
BtgI C'CryG_G

Cuts at:      0     209     408 
   Size:        209     199
 
EaeI y'GGCC_r

Cuts at:      0      27     264     408 
   Size:         27     237     144

  Fragments arranged by size: 

                 237     144      27
 
EarI CTCTTCn'nnn_

Cuts at:      0      80     408 
   Size:         80     328
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     206     408 
   Size:        206     202
 
EcoRI G'AATT_C

Cuts at:      0     178     408 
   Size:        178     230
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141     282     314     408 
   Size:        109      32     141      32      94

  Fragments arranged by size: 

                 141     109      94      32      32
 
MscI TGG'CCA

Cuts at:      0      29     408 
   Size:         29     379
 
MslI CAynn'nnrTG

Cuts at:      0     311     408 
   Size:        311      97
 
NcoI C'CATG_G

Cuts at:      0     209     408 
   Size:        209     199
 
PstI C_TGCA'G

Cuts at:      0     290     408 
   Size:        290     118
 
SexAI A'CCwGG_T

Cuts at:      0     294     408 
   Size:        294     114
 
SfcI C'TryA_G

Cuts at:      0     286     408 
   Size:        286     122
 
StyI C'CwwG_G

Cuts at:      0     209     408 
   Size:        209     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      87     157     408 
   Size:         87      70     251

  Fragments arranged by size: 

                 251      87      70
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     207     408 
   Size:        207     201
 

 Enzymes that do cut:

    ApaLI     ApoI     BanI     BbsI Bme1580I    BseYI  BsiHKAI     BsmI
 Bsp1286I     BtgI     EaeI     EarI     EciI    EcoRI    Hin4I     MscI
     MslI     NcoI     PstI    SexAI     SfcI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI