(Circular) (Six-base) MAPSORT of: pFN21AE6294.seq Check: 7367 from: 1 to: 5353 pFN21AE6294 5353 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3163 278 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2696 1937 Size: 759 4594 Acc65I G'GTAC_C Cuts at: 2674 2674 Size: 5353 AclI AA'CG_TT Cuts at: 1563 3480 3853 1563 Size: 1917 373 3063 Fragments arranged by size: 3063 1917 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2557 3416 4800 5068 1632 Size: 925 859 1384 268 1917 Fragments arranged by size: 1917 1384 925 859 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5156 AflIII A'CryG_T Cuts at: 1203 4252 5229 1203 Size: 3049 977 1327 Fragments arranged by size: 3049 1327 977 AgeI A'CCGG_T Cuts at: 2279 4184 2279 Size: 1905 3448 AhdI GACnn_n'nnGTC Cuts at: 4082 4082 Size: 5353 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5353 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5353 AlwNI CAG_nnn'CTG Cuts at: 2194 3096 4668 2194 Size: 902 1572 2879 Fragments arranged by size: 2879 1572 902 ApaLI G'TGCA_C Cuts at: 2078 3410 4566 2078 Size: 1332 1156 2865 Fragments arranged by size: 2865 1332 1156 ApoI r'AATT_y Cuts at: 1493 2662 2888 3040 5149 1493 Size: 1169 226 152 2109 1697 Fragments arranged by size: 2109 1697 1169 226 152 AseI AT'TA_AT Cuts at: 160 1032 3907 160 Size: 872 2875 1606 Fragments arranged by size: 2875 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 AvaI C'yCGr_G Cuts at: 1870 1943 2517 2678 1870 Size: 73 574 161 4545 Fragments arranged by size: 4545 574 161 73 BamHI G'GATC_C Cuts at: 2683 3050 2683 Size: 367 4986 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2674 4129 618 Size: 325 217 627 887 1455 1842 Fragments arranged by size: 1842 1455 887 627 325 217 BanII G_rGCy'C Cuts at: 1391 2107 2201 2626 2672 1391 Size: 716 94 425 46 4072 Fragments arranged by size: 4072 716 425 94 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2563 2588 928 Size: 971 664 25 3693 Fragments arranged by size: 3693 971 664 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3544 3578 1903 Size: 34 1607 34 3678 Fragments arranged by size: 3678 1607 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3245 4455 3245 Size: 1210 4143 BclI T'GATC_A Cuts at: 1548 2323 1548 Size: 775 4578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3964 136 Size: 107 122 71 631 605 2292 1525 Fragments arranged by size: 2292 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5348 5348 Size: 5353 BlpI GC'TnA_GC Cuts at: 2770 2770 Size: 5353 Bme1580I G_kGCm'C Cuts at: 1790 2082 2157 3414 4570 1790 Size: 292 75 1257 1156 2573 Fragments arranged by size: 2573 1257 1156 292 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3084 4042 442 Size: 416 53 83 399 24 1667 958 1753 Fragments arranged by size: 1753 1667 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 2179 2363 4013 1239 Size: 882 58 184 1650 2579 Fragments arranged by size: 2579 1650 882 184 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3478 4343 4641 3478 Size: 865 298 4190 Fragments arranged by size: 4190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4016 882 Size: 617 2517 2219 Fragments arranged by size: 2517 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2083 3144 493 Size: 711 879 1061 2702 Fragments arranged by size: 2702 1061 879 711 BsaBI GATnn'nnATC Cuts at: 2453 5347 2453 Size: 2894 2459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3160 3542 4177 275 Size: 53 83 186 936 1627 382 635 1451 Fragments arranged by size: 1627 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2170 2279 3786 4184 4458 4605 5016 2170 Size: 109 1507 398 274 147 411 2507 Fragments arranged by size: 2507 1507 411 398 274 147 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 1178 Size: 30 165 30 664 30 4434 Fragments arranged by size: 4434 664 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5353 BseYI C'CCAG_C Cuts at: 2143 2182 2419 4556 5052 2143 Size: 39 237 2137 496 2444 Fragments arranged by size: 2444 2137 496 237 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4780 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3564 4592 1540 Size: 275 186 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 3414 3499 4570 2082 Size: 1332 85 1071 2865 Fragments arranged by size: 2865 1332 1071 85 BsmI GAATG_Cn' Cuts at: 2864 2957 2864 Size: 93 5260 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2107 2157 2201 2626 2672 3414 Size: 399 292 25 50 44 425 46 742 Cuts at: 3414 3499 4570 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 425 399 292 85 50 46 44 25 BspEI T'CCGG_A Cuts at: 5016 5016 Size: 5353 BspHI T'CATG_A Cuts at: 3242 4972 3242 Size: 1730 3623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2532 2708 844 Size: 1688 176 3489 Fragments arranged by size: 3489 1688 176 BsrBI CCG'CTC Cuts at: 1394 1823 3240 1394 Size: 429 1417 3507 Fragments arranged by size: 3507 1417 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2547 3848 4022 65 Size: 1183 1299 1301 174 1396 Fragments arranged by size: 1396 1301 1299 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2279 3997 4184 1700 Size: 110 469 1718 187 2869 Fragments arranged by size: 2869 1718 469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5353 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5353 BssSI C'ACGA_G Cuts at: 2459 3413 4425 2459 Size: 954 1012 3387 Fragments arranged by size: 3387 1012 954 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5353 BstBI TT'CG_AA Cuts at: 4157 4157 Size: 5353 BstEII G'GTnAC_C Cuts at: 1184 2275 1184 Size: 1091 4262 BstYI r'GATC_y Cuts at: 1972 2683 3050 3435 3452 4904 4990 5002 5348 Size: 711 367 385 17 1452 86 12 346 Cuts at: 5348 1972 Size: 1977 Fragments arranged by size: 1977 1452 711 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2616 513 Size: 552 939 66 546 3250 Fragments arranged by size: 3250 939 552 546 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4614 Fragments arranged by size: 4614 683 56 BtsI GCAGTG_nn' Cuts at: 2423 2869 3663 3683 2423 Size: 446 794 20 4093 Fragments arranged by size: 4093 794 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5353 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5318 DraI TTT'AAA Cuts at: 2657 2657 Size: 5353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4360 809 Size: 507 3044 1802 Fragments arranged by size: 3044 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2800 3689 Size: 54 1425 50 275 18 411 559 889 Cuts at: 3689 5318 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 559 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2800 1537 Size: 275 988 4090 Fragments arranged by size: 4090 988 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2396 3283 1353 Size: 884 159 887 3423 Fragments arranged by size: 3423 887 884 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3940 4314 4460 387 Size: 1064 138 2351 374 146 1280 Fragments arranged by size: 2351 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2179 2363 2557 3416 4013 4800 Size: 393 489 58 184 194 859 597 787 Cuts at: 4800 5068 1239 Size: 268 1524 Fragments arranged by size: 1524 859 787 597 489 393 268 194 184 58 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 5353 EcoO109I rG'GnC_Cy Cuts at: 2521 2521 Size: 5353 EcoRI G'AATT_C Cuts at: 2662 3040 2662 Size: 378 4975 FspI TGC'GCA Cuts at: 3859 3859 Size: 5353 HaeII r_GCGC'y Cuts at: 1678 2444 4500 1678 Size: 766 2056 2531 Fragments arranged by size: 2531 2056 766 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2490 2522 3994 4026 4068 Size: 32 135 32 918 32 1472 32 42 Cuts at: 4068 4100 1373 Size: 32 2626 Fragments arranged by size: 2626 1472 918 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2044 2697 2943 669 Size: 988 281 106 653 246 3079 Fragments arranged by size: 3079 988 653 281 246 106 HindIII A'AGCT_T Cuts at: 748 748 Size: 5353 HpaI GTT'AAC Cuts at: 2943 2943 Size: 5353 KpnI G_GTAC'C Cuts at: 2678 2678 Size: 5353 MfeI C'AATT_G Cuts at: 2952 2952 Size: 5353 MluI A'CGCG_T Cuts at: 5229 5229 Size: 5353 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4467 4651 1305 Size: 3162 184 2007 Fragments arranged by size: 3162 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3531 Fragments arranged by size: 3531 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2459 2474 3311 3670 3829 518 Size: 648 909 384 15 837 359 159 2042 Fragments arranged by size: 2042 909 837 648 384 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2120 2269 2297 2383 2769 3446 4594 4839 Size: 514 149 28 86 386 677 1148 245 Cuts at: 4839 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 514 386 245 149 86 28 NcoI C'CATG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 3796 Fragments arranged by size: 3796 939 552 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 5353 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5353 NotI GC'GGCC_GC Cuts at: 2800 2800 Size: 5353 NspI r_CATG'y Cuts at: 1132 1229 2711 4256 1132 Size: 97 1482 1545 2229 Fragments arranged by size: 2229 1545 1482 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5353 PciI A'CATG_T Cuts at: 4252 4252 Size: 5353 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5353 PmeI GTTT'AAAC Cuts at: 2657 2657 Size: 5353 PmlI CAC'GTG Cuts at: 2083 2083 Size: 5353 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 4935 4967 3422 Size: 32 1481 32 3808 Fragments arranged by size: 3808 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2521 2521 Size: 5353 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5353 PsiI TTA'TAA Cuts at: 2923 2923 Size: 5353 PstI C_TGCA'G Cuts at: 830 2705 830 Size: 1875 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5353 PvuII CAG'CTG Cuts at: 2120 2269 2297 2383 2120 Size: 149 28 86 5090 Fragments arranged by size: 5090 149 86 28 SalI G'TCGA_C Cuts at: 1936 2695 1936 Size: 759 4594 SbfI CC_TGCA'GG Cuts at: 2705 2705 Size: 5353 ScaI AGT'ACT Cuts at: 3601 5313 3601 Size: 1712 3641 SexAI A'CCwGG_T Cuts at: 2590 2590 Size: 5353 SfcI C'TryA_G Cuts at: 826 1045 2224 2292 2701 3836 4517 4708 826 Size: 219 1179 68 409 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 681 409 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5353 SmaI CCC'GGG Cuts at: 1872 2519 2680 1872 Size: 647 161 4545 Fragments arranged by size: 4545 647 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3457 4358 4620 820 Size: 197 926 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3144 493 Size: 2651 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5353 SphI G_CATG'C Cuts at: 1132 2711 1132 Size: 1579 3774 SspI AAT'ATT Cuts at: 5 52 3277 5 Size: 47 3225 2081 Fragments arranged by size: 3225 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5353 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 2070 2482 2792 513 Size: 552 939 31 35 412 310 3074 Fragments arranged by size: 3074 939 552 412 310 35 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3380 3397 3550 3735 4180 325 Size: 1255 1800 17 153 185 445 1498 Fragments arranged by size: 1800 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3599 5311 96 Size: 274 80 33 51 1443 1622 1712 138 Fragments arranged by size: 1712 1622 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 2302 2334 5313 5345 2067 Size: 32 203 32 2979 32 2075 Fragments arranged by size: 2979 2075 203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5353 XbaI T'CTAG_A Cuts at: 2689 2689 Size: 5353 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2258 2258 Size: 5353 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5353 XmaI C'CCGG_G Cuts at: 1870 2517 2678 1870 Size: 647 161 4545 Fragments arranged by size: 4545 647 161 XmnI GAAnn'nnTTC Cuts at: 3482 3482 Size: 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3161 276 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI