(Linear) (Six-base) MAPSORT of: KIEE6294.seq Check: 2001 from: 1 to: 648 KIEE6294.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 552 648 Size: 552 96 AgeI A'CCGG_T Cuts at: 0 274 648 Size: 274 374 AlwNI CAG_nnn'CTG Cuts at: 0 189 648 Size: 189 459 ApaLI G'TGCA_C Cuts at: 0 73 648 Size: 73 575 AvaI C'yCGr_G Cuts at: 0 512 648 Size: 512 136 BanII G_rGCy'C Cuts at: 0 102 196 621 648 Size: 102 94 425 27 Fragments arranged by size: 425 102 94 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 558 583 648 Size: 558 25 65 Fragments arranged by size: 558 65 25 BclI T'GATC_A Cuts at: 0 318 648 Size: 318 330 Bme1580I G_kGCm'C Cuts at: 0 77 152 648 Size: 77 75 496 Fragments arranged by size: 496 77 75 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 174 358 648 Size: 116 58 184 290 Fragments arranged by size: 290 184 116 58 BsaAI yAC'GTr Cuts at: 0 78 648 Size: 78 570 BsaBI GATnn'nnATC Cuts at: 0 448 648 Size: 448 200 BsaWI w'CCGG_w Cuts at: 0 165 274 648 Size: 165 109 374 Fragments arranged by size: 374 165 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 62 92 648 Size: 62 30 556 Fragments arranged by size: 556 62 30 BseYI C'CCAG_C Cuts at: 0 138 177 414 648 Size: 138 39 237 234 Fragments arranged by size: 237 234 138 39 BsiHKAI G_wGCw'C Cuts at: 0 77 648 Size: 77 571 Bsp1286I G_dGCh'C Cuts at: 0 77 102 152 196 621 648 Size: 77 25 50 44 425 27 Fragments arranged by size: 425 77 50 44 27 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 527 648 Size: 527 121 BsrDI GCAATG_nn' Cuts at: 0 542 648 Size: 542 106 BsrFI r'CCGG_y Cuts at: 0 274 648 Size: 274 374 BssSI C'ACGA_G Cuts at: 0 454 648 Size: 454 194 BstEII G'GTnAC_C Cuts at: 0 270 648 Size: 270 378 BtgI C'CryG_G Cuts at: 0 65 611 648 Size: 65 546 37 Fragments arranged by size: 546 65 37 BtsI GCAGTG_nn' Cuts at: 0 418 648 Size: 418 230 EaeI y'GGCC_r Cuts at: 0 236 648 Size: 236 412 EarI CTCTTCn'nnn_ Cuts at: 0 232 391 648 Size: 232 159 257 Fragments arranged by size: 257 232 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 116 174 358 552 648 Size: 116 58 184 194 96 Fragments arranged by size: 194 184 116 96 58 EcoNI CCTnn'n_nnAGG Cuts at: 0 349 648 Size: 349 299 EcoO109I rG'GnC_Cy Cuts at: 0 516 648 Size: 516 132 HaeII r_GCGC'y Cuts at: 0 439 648 Size: 439 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 485 517 648 Size: 485 32 131 Fragments arranged by size: 485 131 32 HincII GTy'rAC Cuts at: 0 39 648 Size: 39 609 MslI CAynn'nnrTG Cuts at: 0 70 454 469 648 Size: 70 384 15 179 Fragments arranged by size: 384 179 70 15 MspA1I CmG'CkG Cuts at: 0 115 264 292 378 648 Size: 115 149 28 86 270 Fragments arranged by size: 270 149 115 86 28 NcoI C'CATG_G Cuts at: 0 65 648 Size: 65 583 PmlI CAC'GTG Cuts at: 0 78 648 Size: 78 570 PpuMI rG'GwC_Cy Cuts at: 0 516 648 Size: 516 132 PvuII CAG'CTG Cuts at: 0 115 264 292 378 648 Size: 115 149 28 86 270 Fragments arranged by size: 270 149 115 86 28 SexAI A'CCwGG_T Cuts at: 0 585 648 Size: 585 63 SfcI C'TryA_G Cuts at: 0 219 287 648 Size: 219 68 361 Fragments arranged by size: 361 219 68 SmaI CCC'GGG Cuts at: 0 514 648 Size: 514 134 StyI C'CwwG_G Cuts at: 0 30 65 477 648 Size: 30 35 412 171 Fragments arranged by size: 412 171 35 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 62 94 297 329 648 Size: 62 32 203 32 319 Fragments arranged by size: 319 203 62 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 253 648 Size: 253 395 XmaI C'CCGG_G Cuts at: 0 512 648 Size: 512 136 Enzymes that do cut: AcuI AgeI AlwNI ApaLI AvaI BanII BbsI BclI Bme1580I BpmI BsaAI BsaBI BsaWI BsaXI BseYI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MslI MspA1I NcoI PmlI PpuMI PvuII SexAI SfcI SmaI StyI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaHI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI