################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII HapII HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 40 CCGC CCGC(-3/-1) AcyI 31 GACGCC G[GA]^CG[CT]C AlwI 60 GATCC GGATC(4/5) Aor51HI 149 AGCGCT AGC^GCT BanII 169 GAGCCC G[GA]GC[CT]^C BbvI 137 GCTGC GCAGC(8/12) BglII 11 AGATCT A^GATCT BmyI 169 GAGCCC G[AGT]GC[ACT]^C BsaBI 54 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 31 GACGCC G[GA]^CG[CT]C BseRI 166 GAGGAG GAGGAG(10/8) BsmFI 26 GGGAC GGGAC(10/14) Bsp1286I 169 GAGCCC G[AGT]GC[ACT]^C BspMI 191 ACCTGC ACCTGC(4/8) BstAPI 8 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 150 GCGC GCG^C DraII 188 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 161 CCATGG C^C[GA][CT]GG Eco47III 149 AGCGCT AGC^GCT EcoO109I 188 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 39 CCCGC CCCGC(4/6) Fnu4HI 137 GCTGC GC^[ACGT]GC HaeII 149 AGCGCT [GA]GCGC^[CT] HgaI 31 GACGC GACGC(5/10) HhaI 150 GCGC GCG^C Hin1I 31 GACGCC G[GA]^CG[CT]C HinP1I 150 GCGC G^CGC ItaI 137 GCTGC GC^[ACGT]GC MamI 54 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 57 GAAGA GAAGA(8/7) MspA1I 40 CCGCTG C[AC]G^C[GT]G MwoI 8 GCAAGATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 161 CCATGG C^CATGG NlaIII 162 CATG CATG^ PflMI 95 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 188 AGGACCT [GA]G^G[AT]CC[CT] SfaNI 80 GCATC GCATC(5/9) TaqI 125 TCGA T^CGA TseI 137 GCTGC G^C[AT]GC TthHB8I 125 TCGA T^CGA Van91I 95 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 157 GGACC G^G[AT]CC 189 GGACC BsiYI 63 CCAACCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 95 CCATCAAGTGG BslI 63 CCAACCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 95 CCATCAAGTGG BssKI 84 CCTGG ^CC[ACGT]GG 174 CCTGG BstF5I 81 CATCC GGATG(2/0) 53 GGATG BstNI 84 CCTGG CC^[AT]GG 174 CCTGG BstYI 11 AGATCT [GA]^GATC[CT] 59 AGATCC Cfr13I 157 GGACC G^G[ACGT]CC 189 GGACC DpnII 12 GATC ^GATC 60 GATC EcoRII 84 CCTGG ^CC[AT]GG 174 CCTGG EcoT14I 68 CCAAGG C^C[AT][AT]GG 161 CCATGG FokI 81 CATCC GGATG(9/13) 53 GGATG MboI 12 GATC ^GATC 60 GATC MflI 11 AGATCT [GA]^GATC[CT] 59 AGATCC MnlI 120 CCTC CCTC(7/6) 166 GAGG MvaI 84 CCTGG CC^[AT]GG 174 CCTGG NdeII 12 GATC ^GATC 60 GATC NlaIV 157 GGACCC GG[ACGT]^[ACGT]CC 168 GGAGCC Sau3AI 12 GATC ^GATC 60 GATC Sau96I 157 GGACC G^G[ACGT]CC 189 GGACC ScrFI 84 CCTGG CC^[ACGT]GG 174 CCTGG StyI 68 CCAAGG C^C[AT][AT]GG 161 CCATGG XhoII 11 AGATCT [GA]^GATC[CT] 59 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### BsaJI HphI