################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 120 GACGCC G[GA]^CG[CT]C Aor51HI 267 AGCGCT AGC^GCT AvaII 27 GGACC G^G[AT]CC BbsI 779 GAAGAC GAAGAC(2/6) BciVI 599 GGATAC GTATCC(6/5) BfaI 328 CTAG C^TAG BmyI 635 GTGCCC G[AGT]GC[ACT]^C BpuAI 779 GAAGAC GAAGAC(2/6) BsaHI 120 GACGCC G[GA]^CG[CT]C BsiWI 17 CGTACG C^GTACG Bsp1286I 635 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 392 TGTACA T^GTACA BspMI 178 GCAGGT ACCTGC(4/8) BsrGI 392 TGTACA T^GTACA BssSI 259 CTCGTG CACGAG(-5/-1) CfoI 268 GCGC GCG^C Cfr13I 27 GGACC G^G[ACGT]CC DdeI 167 CTCAG C^T[ACGT]AG DraI 313 TTTAAA TTT^AAA Eco47III 267 AGCGCT AGC^GCT FauI 131 GCGGG CCCGC(4/6) HaeII 267 AGCGCT [GA]GCGC^[CT] HhaI 268 GCGC GCG^C Hin1I 120 GACGCC G[GA]^CG[CT]C HinP1I 268 GCGC G^CGC HincII 404 GTCAAC GT[CT]^[GA]AC HindII 404 GTCAAC GT[CT]^[GA]AC HphI 208 TCACC GGTGA(8/7) MaeI 328 CTAG C^TAG MaeIII 367 GTGAC ^GT[ACGT]AC MseI 314 TTAA T^TAA NlaIII 220 CATG CATG^ PleI 230 GACTC GAGTC(4/5) PstI 377 CTGCAG CTGCA^G Sau96I 27 GGACC G^G[ACGT]CC SfaNI 306 GATGC GCATC(5/9) SfcI 377 CTGCAG C^T[GA][CT]AG SmaI 125 CCCGGG CCC^GGG SmlI 58 CTCAAG C^T[CT][GA]AG SspBI 392 TGTACA T^GTACA Tru9I 314 TTAA T^TAA Tsp45I 367 GTGAC ^GT[GC]AC TspRI 527 CAGTG CA[GC]TG[ACGT][ACGT]^ XmaCI 125 CCCGGG C^CCGGG XmaI 125 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 277 AAATTT [GA]^AATT[CT] 334 GAATTT AlwI 466 GATCC GGATC(4/5) 440 GGATC ApoI 277 AAATTT [GA]^AATT[CT] 334 GAATTT AvaI 8 CTCGGG C^[CT]CG[GA]G 125 CCCGGG BbvI 652 GCAGC GCAGC(8/12) 376 GCTGC BseRI 22 GAGGAG GAGGAG(10/8) 67 GAGGAG BsiEI 89 CGGCCG CG[GA][CT]^CG 153 CGGCCG BsmAI 13 GAGAC GTCTC(1/5) 56 GTCTC BsmBI 55 CGTCTC CGTCTC(1/5) 13 GAGACG BsmFI 283 GGGAC GGGAC(10/14) 755 GGGAC BsoBI 8 CTCGGG C^[CT]CG[GA]G 125 CCCGGG BsrDI 396 CATTGC GCAATG(2/0) 607 GCAATG BstF5I 531 GGATG GGATG(2/0) 682 GGATG BstNI 45 CCAGG CC^[AT]GG 451 CCAGG EagI 89 CGGCCG C^GGCCG 153 CGGCCG EclXI 89 CGGCCG C^GGCCG 153 CGGCCG Eco52I 89 CGGCCG C^GGCCG 153 CGGCCG EcoRII 45 CCAGG ^CC[AT]GG 451 CCAGG FokI 531 GGATG GGATG(9/13) 682 GGATG HgaI 120 GACGC GACGC(5/10) 54 GCGTC MaeII 16 ACGT A^CGT 573 ACGT MboII 779 GAAGA GAAGA(8/7) 489 TCTTC MvaI 45 CCAGG CC^[AT]GG 451 CCAGG TaqI 80 TCGA T^CGA 225 TCGA TseI 376 GCTGC G^C[AT]GC 652 GCAGC TthHB8I 80 TCGA T^CGA 225 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AfaI AluI BcnI BsaJI BsiYI BslI BssKI DpnII EaeI Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MnlI MspI MwoI NciI NdeII RsaI Sau3AI ScrFI TfiI Tsp509I