(Circular) (Six-base) MAPSORT of: pFN21AE5362.seq Check: 5774 from: 1 to: 5530 pFN21AE5362 5530 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2174 Size: 5530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3593 4977 5245 1632 Size: 1961 1384 268 1917 Fragments arranged by size: 1961 1917 1384 268 AfeI AGC'GCT Cuts at: 2274 2274 Size: 5530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 4429 5406 1203 Size: 3226 977 1327 Fragments arranged by size: 3226 1327 977 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 3273 4845 3273 Size: 1572 3958 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2282 2339 2839 3065 3217 5326 1493 Size: 789 57 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 789 500 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2130 2855 1870 Size: 73 70 117 725 4545 Fragments arranged by size: 4545 725 117 73 70 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4306 618 Size: 325 217 627 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2849 1391 Size: 1458 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2791 928 Size: 971 892 3667 Fragments arranged by size: 3667 971 892 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 1903 Size: 34 1784 34 3678 Fragments arranged by size: 3678 1784 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2598 3422 4632 2598 Size: 824 1210 3496 Fragments arranged by size: 3496 1210 824 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4141 136 Size: 107 122 71 631 605 337 2132 1525 Fragments arranged by size: 2132 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5525 5525 Size: 5530 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 2644 3591 4747 1790 Size: 854 947 1156 2573 Fragments arranged by size: 2573 1156 947 854 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 4219 442 Size: 416 53 83 399 24 1844 958 1753 Fragments arranged by size: 1844 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 2187 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4190 1239 Size: 2951 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 3655 4520 4818 2048 Size: 1607 865 298 2760 Fragments arranged by size: 2760 1607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4193 882 Size: 617 2694 2219 Fragments arranged by size: 2694 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3321 493 Size: 711 2117 2702 Fragments arranged by size: 2702 2117 711 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 3337 3719 4354 Size: 53 83 186 936 593 1211 382 635 Cuts at: 4354 275 Size: 1451 Fragments arranged by size: 1451 1211 936 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3963 4361 4635 4782 5193 3963 Size: 398 274 147 411 4300 Fragments arranged by size: 4300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5305 Fragments arranged by size: 5305 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2087 1186 Size: 856 45 4629 Fragments arranged by size: 4629 856 45 BseYI C'CCAG_C Cuts at: 4733 5229 4733 Size: 496 5034 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4957 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 2161 2980 3741 4769 1540 Size: 275 186 96 64 819 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 275 186 96 64 BsiHKAI G_wGCw'C Cuts at: 3591 3676 4747 3591 Size: 85 1071 4374 Fragments arranged by size: 4374 1071 85 BsiWI C'GTAC_G Cuts at: 2022 2022 Size: 5530 BsmI GAATG_Cn' Cuts at: 3041 3134 3041 Size: 93 5437 BsmBI CGTCTCn'nnnn_ Cuts at: 2012 2066 2012 Size: 54 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2644 2849 3591 3676 4747 1391 Size: 399 854 205 742 85 1071 2174 Fragments arranged by size: 2174 1071 854 742 399 205 85 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 3419 5149 3419 Size: 1730 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2885 844 Size: 1330 711 3489 Fragments arranged by size: 3489 1330 711 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2400 2619 4025 4199 65 Size: 1183 1152 219 1406 174 1396 Fragments arranged by size: 1406 1396 1183 1152 219 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 3229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 2264 3590 4602 2264 Size: 1326 1012 3192 Fragments arranged by size: 3192 1326 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5530 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525 Size: 888 367 385 17 1452 86 12 346 Cuts at: 5525 1972 Size: 1977 Fragments arranged by size: 1977 1452 888 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4978 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2157 487 Size: 683 56 931 3860 Fragments arranged by size: 3860 931 683 56 BtsI GCAGTG_nn' Cuts at: 3046 3840 3860 3046 Size: 794 20 4716 Fragments arranged by size: 4716 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2514 2549 529 Size: 35 1950 35 3510 Fragments arranged by size: 3510 1950 35 35 DraI TTT'AAA Cuts at: 2320 2834 2320 Size: 514 5016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 809 Size: 507 3221 1802 Fragments arranged by size: 3221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2158 2453 Size: 54 1425 50 275 18 264 64 295 Cuts at: 2453 2717 2977 3866 5495 8 Size: 264 260 889 1629 43 Fragments arranged by size: 1629 1425 889 295 275 264 264 260 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2094 2158 2977 1537 Size: 275 282 64 819 4090 Fragments arranged by size: 4090 819 282 275 64 EarI CTCTTCn'nnn_ Cuts at: 1353 3460 1353 Size: 2107 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3593 4190 4977 5245 1239 Size: 393 1961 597 787 268 1524 Fragments arranged by size: 1961 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2839 3217 2839 Size: 378 5152 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 2276 4677 1678 Size: 598 2401 2531 Fragments arranged by size: 2531 2401 598 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2612 2644 4171 Size: 32 135 32 887 32 121 32 1527 Cuts at: 4171 4203 4245 4277 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 887 135 121 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2411 2874 3120 669 Size: 988 281 473 463 246 3079 Fragments arranged by size: 3079 988 473 463 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5530 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4644 4828 1305 Size: 3339 184 2007 Fragments arranged by size: 3339 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3708 Fragments arranged by size: 3708 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3488 3847 4006 518 Size: 648 2322 359 159 2042 Fragments arranged by size: 2322 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2946 3623 4771 5016 1606 Size: 1340 677 1148 245 2120 Fragments arranged by size: 2120 1340 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4978 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2888 4433 1132 Size: 97 1659 1545 2229 Fragments arranged by size: 2229 1659 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5530 PciI A'CATG_T Cuts at: 4429 4429 Size: 5530 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5530 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PstI C_TGCA'G Cuts at: 830 2386 2882 830 Size: 1556 496 3478 Fragments arranged by size: 3478 1556 496 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 3778 5490 3778 Size: 1712 3818 SfcI C'TryA_G Cuts at: 826 1045 2382 2878 4013 4694 4885 826 Size: 219 1337 496 1135 681 191 1471 Fragments arranged by size: 1471 1337 1135 681 496 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2132 2857 1872 Size: 260 725 4545 Fragments arranged by size: 4545 725 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 3634 4535 4797 820 Size: 197 926 120 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2888 1132 Size: 1756 3774 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2969 513 Size: 552 1904 3074 Fragments arranged by size: 3074 1904 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 3557 3574 3727 3912 4357 325 Size: 1255 504 1473 17 153 185 445 1498 Fragments arranged by size: 1498 1473 1255 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 2817 3776 Size: 274 80 33 51 1443 420 420 959 Cuts at: 3776 5488 96 Size: 1712 138 Fragments arranged by size: 1712 1443 959 420 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 5490 5522 2205 Size: 32 3253 32 2213 Fragments arranged by size: 3253 2213 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5530 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5530 XmaI C'CCGG_G Cuts at: 1870 2130 2855 1870 Size: 260 725 4545 Fragments arranged by size: 4545 725 260 XmnI GAAnn'nnTTC Cuts at: 3659 3659 Size: 5530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI