################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AccIII AclI AflII AflIII AgeI AhdI ApaI AscI AspEI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI HapII HgaI HpaI HpaII KasI KspI MamI MluI MroI MspI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 2840 GACGTC GACGT^C Acc65I 1666 GGTACC G^GTACC AccI 1922 GTCGAC GT^[AC][GT]AC AccII 2294 CGCG CG^CG AcsI 187 GAATTC [GA]^AATT[CT] AcyI 2840 GACGTC G[GA]^CG[CT]C Alw44I 533 GTGCAC G^TGCAC AocI 875 CCTCAGG CC^T[ACGT]AGG Aor51HI 1222 AGCGCT AGC^GCT ApaLI 533 GTGCAC G^TGCAC ApoI 187 GAATTC [GA]^AATT[CT] Asp718I 1666 GGTACC G^GTACC AspI 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1787 CCCGAG C^[CT]CG[GA]G AviII 1875 TGCGCA TGC^GCA AvrII 363 CCTAGG C^CTAGG BalI 1803 TGGCCA TGG^CCA BamHI 104 GGATCC G^GATCC BglI 1119 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1033 AGATCT A^GATCT BlnI 363 CCTAGG C^CTAGG BsaHI 2840 GACGTC G[GA]^CG[CT]C BsmBI 2838 GAGACG CGTCTC(1/5) BsoBI 1787 CCCGAG C^[CT]CG[GA]G BstUI 2294 CGCG CG^CG BstXI 291 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 875 CCTCAGG CC^T[ACGT]AGG DraII 2616 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 1084 CCACGG C^C[GA][CT]GG Eco47III 1222 AGCGCT AGC^GCT Eco81I 875 CCTCAGG CC^T[ACGT]AGG EcoNI 662 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2616 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 187 GAATTC G^AATTC EcoT22I 1484 ATGCAT ATGCA^T FspI 1875 TGCGCA TGC^GCA HaeII 1222 AGCGCT [GA]GCGC^[CT] Hin1I 2840 GACGTC G[GA]^CG[CT]C KpnI 1666 GGTACC GGTAC^C MaeII 2841 ACGT A^CGT MfeI 2699 CAATTG C^AATTG MluNI 1803 TGGCCA TGG^CCA MscI 1803 TGGCCA TGG^CCA MunI 2699 CAATTG C^AATTG MvnI 2294 CGCG CG^CG NdeI 2913 CATATG CA^TATG NsiI 1484 ATGCAT ATGCA^T PflFI 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2338 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2616 AGGTCCT [GA]G^G[AT]CC[CT] SalI 1922 GTCGAC G^TCGAC SapI 2533 GCTCTTC GCTCTTC(1/4) SbfI 148 CCTGCAGG CCTGCA^GG SexAI 2463 ACCTGGT A^CC[AT]GGT Sse8387I 148 CCTGCAGG CCTGCA^GG SspI 342 AATATT AAT^ATT StuI 1262 AGGCCT AGG^CCT Tth111I 2376 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2338 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2912 CCATATGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 566 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 2224 CAGAGACTG Asp700I 183 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2641 GAACTGATTC BanI 1666 GGTACC G^G[CT][GA]CC 2112 GGCACC BbsI 2779 GAAGAC GAAGAC(2/6) 2843 GTCTTC BbvCI 780 CCTCAGC CCTCAGC(-5/-2) 2158 CCTCAGC BpuAI 2779 GAAGAC GAAGAC(2/6) 2843 GTCTTC BsaI 539 GGTCTC GGTCTC(1/5) 1007 GGTCTC Bsp1407I 2061 TGTACA T^GTACA 2710 TGTACA BspHI 1677 TCATGA T^CATGA 2238 TCATGA BsrGI 2061 TGTACA T^GTACA 2710 TGTACA BstYI 104 GGATCC [GA]^GATC[CT] 1033 AGATCT CfoI 1223 GCGC GCG^C 1876 GCGC EaeI 1072 CGGCCA [CT]^GGCC[GA] 1803 TGGCCA FauI 1989 CCCGC CCCGC(4/6) 2295 GCGGG HhaI 1223 GCGC GCG^C 1876 GCGC HinP1I 1223 GCGC G^CGC 1876 GCGC HindIII 911 AAGCTT A^AGCTT 1976 AAGCTT MflI 104 GGATCC [GA]^GATC[CT] 1033 AGATCT NspI 1931 ACATGC [GA]CATG^[CT] 2064 ACATGC PstI 149 CTGCAG CTGCA^G 2447 CTGCAG RcaI 1677 TCATGA T^CATGA 2238 TCATGA SacI 28 GAGCTC GAGCT^C 2531 GAGCTC SspBI 2061 TGTACA T^GTACA 2710 TGTACA XhoII 104 GGATCC [GA]^GATC[CT] 1033 AGATCT XmnI 183 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2641 GAACTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AseI AsnI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI Cac8I Cfr13I DdeI DpnII DraIII EarI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I