Restriction map for
pFN21AE5673
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI HgaI Hin1I
HpaI KasI Ksp632I KspI MamI
MluI MroI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 962 GGTACC G^GTACC
AccII 31 CGCG CG^CG
AclI 883 AACGTT AA^CGTT
AcsI 1088 AAATTT [GA]^AATT[CT]
AlwNI 466 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1088 AAATTT [GA]^AATT[CT]
Asp718I 962 GGTACC G^GTACC
AviII 9 TGCGCA TGC^GCA
BcnI 131 CCGGG CC^[GC]GG
BfaI 1516 CTAG C^TAG
BglI 1003 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 106 TACGTG [CT]AC^GT[GA]
BsaI 1027 GGTCTC GGTCTC(1/5)
BsgI 1037 GTGCAG GTGCAG(16/14)
BsmBI 398 CGTCTC CGTCTC(1/5)
Bsp1407I 312 TGTACA T^GTACA
BspHI 254 TCATGA T^CATGA
BspMI 713 GCAGGT ACCTGC(4/8)
BsrGI 312 TGTACA T^GTACA
BssHII 30 GCGCGC G^CGCGC
BssSI 157 CACGAG CACGAG(-5/-1)
BstAPI 1039 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 31 CGCG CG^CG
BstXI 805 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 681 GGATCT [GA]^GATC[CT]
DraI 1083 TTTAAA TTT^AAA
DraIII 1149 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1433 CCACGG C^C[GA][CT]GG
FspI 9 TGCGCA TGC^GCA
HapII 131 CCGG C^CGG
HincII 619 GTTGAC GT[CT]^[GA]AC
HindII 619 GTTGAC GT[CT]^[GA]AC
HpaII 131 CCGG C^CGG
KpnI 962 GGTACC GGTAC^C
MaeI 1516 CTAG C^TAG
MfeI 842 CAATTG C^AATTG
MflI 681 GGATCT [GA]^GATC[CT]
MspI 131 CCGG C^CGG
MunI 842 CAATTG C^AATTG
MvnI 31 CGCG CG^CG
NciI 131 CCGGG CC^[GC]GG
Psp1406I 883 AACGTT AA^CGTT
PstI 296 CTGCAG CTGCA^G
RcaI 254 TCATGA T^CATGA
SacI 250 GAGCTC GAGCT^C
ScaI 1471 AGTACT AGT^ACT
SfcI 296 CTGCAG C^T[GA][CT]AG
SphI 6 GCATGC GCATG^C
SspBI 312 TGTACA T^GTACA
SspI 167 AATATT AAT^ATT
StuI 242 AGGCCT AGG^CCT
XcmI 1013 CCAAAAATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 681 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 1102 GATCC GGATC(4/5)
681 GGATC
AocI 1502 CCTCAGG CC^T[ACGT]AGG
1560 CCTGAGG
AspHI 250 GAGCTC G[AT]GC[AT]^C
460 GTGCTC
AvaII 1062 GGACC G^G[AT]CC
1215 GGTCC
BanI 554 GGTGCC G^G[CT][GA]CC
962 GGTACC
BanII 122 GAGCCC G[GA]GC[CT]^C
250 GAGCTC
BseRI 1169 CTCCTC GAGGAG(10/8)
136 GAGGAG
BsiHKAI 250 GAGCTC G[AT]GC[AT]^C
460 GTGCTC
Bsu36I 1502 CCTCAGG CC^T[ACGT]AGG
1560 CCTGAGG
DpnII 682 GATC ^GATC
1102 GATC
Eco81I 1502 CCTCAGG CC^T[ACGT]AGG
1560 CCTGAGG
EcoT14I 245 CCTTGG C^C[AT][AT]GG
550 CCAAGG
HaeII 467 AGCGCC [GA]GCGC^[CT]
1610 GGCGCT
HindIII 79 AAGCTT A^AGCTT
857 AAGCTT
MboI 682 GATC ^GATC
1102 GATC
MboII 334 GAAGA GAAGA(8/7)
725 TCTTC
MseI 674 TTAA T^TAA
1084 TTAA
NdeII 682 GATC ^GATC
1102 GATC
Sau3AI 682 GATC ^GATC
1102 GATC
StyI 245 CCTTGG C^C[AT][AT]GG
550 CCAAGG
TaqI 662 TCGA T^CGA
1403 TCGA
TfiI 350 GATTC G^A[AT]TC
849 GATTC
Tru9I 674 TTAA T^TAA
1084 TTAA
TthHB8I 662 TCGA T^CGA
1403 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MluNI
MnlI MscI MslI MvaI MwoI
NlaIII NlaIV NspI PflMI PleI
RsaI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI Van91I