(Circular) (Six-base) MAPSORT of: pFN21AE5673.seq Check: 6320 from: 1 to: 6334 pFN21AE5673 6334 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 2967 3655 2967 Size: 688 5646 AclI AA'CG_TT Cuts at: 1563 2889 4461 4834 1563 Size: 1326 1572 373 3063 Fragments arranged by size: 3063 1572 1326 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3457 4397 5781 6049 1632 Size: 1825 940 1384 268 1917 Fragments arranged by size: 1917 1825 1384 940 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 2462 1166 Size: 1296 5038 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2476 4077 5649 2476 Size: 1601 1572 3161 Fragments arranged by size: 3161 1601 1572 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3093 3643 3869 4021 6130 1493 Size: 1600 550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1600 550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3659 1870 Size: 73 1716 4545 Fragments arranged by size: 4545 1716 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 2967 3655 5110 618 Size: 325 217 627 772 408 688 1455 1842 Fragments arranged by size: 1842 1455 772 688 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2131 2259 3653 1391 Size: 740 128 1394 4072 Fragments arranged by size: 4072 1394 740 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3014 4945 136 Size: 107 122 71 631 605 1342 1931 1525 Fragments arranged by size: 1931 1525 1342 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 2564 3382 4395 5551 1790 Size: 774 818 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 818 774 BmgBI CAC'GTC Cuts at: 1318 3253 1318 Size: 1935 4399 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2909 4065 5023 Size: 416 53 83 399 24 1492 1156 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1753 1492 1156 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 2453 2456 2975 4994 1239 Size: 852 362 3 519 2019 2579 Fragments arranged by size: 2579 2019 852 519 362 3 Bpu10I CC'TnA_GC Cuts at: 3129 3129 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4459 5324 5622 4459 Size: 865 298 5171 Fragments arranged by size: 5171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3038 4997 882 Size: 617 1539 1959 2219 Fragments arranged by size: 2219 1959 1539 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 4125 493 Size: 711 909 2012 2702 Fragments arranged by size: 2702 2012 909 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4523 5158 275 Size: 53 83 186 936 2608 382 635 1451 Fragments arranged by size: 2608 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6109 Fragments arranged by size: 6109 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 3165 1186 Size: 970 1009 4355 Fragments arranged by size: 4355 1009 970 BseYI C'CCAG_C Cuts at: 3543 5537 6033 3543 Size: 1994 496 3844 Fragments arranged by size: 3844 1994 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3063 1163 Size: 573 1327 4434 Fragments arranged by size: 4434 1327 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2469 4395 4480 5551 2259 Size: 210 1926 85 1071 3042 Fragments arranged by size: 3042 1926 1071 210 85 BsmI GAATG_Cn' Cuts at: 2346 2663 3055 3175 3845 3938 2346 Size: 317 392 120 670 93 4742 Fragments arranged by size: 4742 670 392 317 120 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2409 2409 Size: 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2259 2469 2564 3382 3653 4395 Size: 399 341 128 210 95 818 271 742 Cuts at: 4395 4480 5551 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 818 742 399 341 271 210 128 95 85 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2259 4223 5953 2259 Size: 1964 1730 2640 Fragments arranged by size: 2640 1964 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2709 3689 844 Size: 1865 980 3489 Fragments arranged by size: 3489 1865 980 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2317 96 Size: 2221 4113 BssHII G'CGCG_C Cuts at: 1925 2035 1925 Size: 110 6224 BssSI C'ACGA_G Cuts at: 2162 4394 5406 2162 Size: 2232 1012 3090 Fragments arranged by size: 3090 2232 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3050 794 Size: 2256 4078 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2817 2817 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2686 3664 4031 4416 4433 5885 5971 5983 Size: 714 978 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 978 714 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3508 3566 3508 Size: 58 6276 BtgI C'CryG_G Cuts at: 513 1065 2004 3438 513 Size: 552 939 1434 3409 Fragments arranged by size: 3409 1434 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2554 3850 4644 4664 2554 Size: 1296 794 20 4224 Fragments arranged by size: 4224 1296 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3090 3638 3090 Size: 548 5786 DraIII CAC_nnn'GTG Cuts at: 3159 3159 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2487 2514 3015 Size: 54 1425 50 275 18 657 27 501 Cuts at: 3015 3781 4670 6299 8 Size: 766 889 1629 43 Fragments arranged by size: 1629 1425 889 766 657 501 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4264 1353 Size: 2911 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2043 4921 5295 5441 387 Size: 1064 138 454 2878 374 146 1280 Fragments arranged by size: 2878 1280 1064 454 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2257 Size: 6334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2453 2456 2975 3457 4397 4994 Size: 393 459 362 3 519 482 940 597 Cuts at: 4994 5781 6049 1239 Size: 787 268 1524 Fragments arranged by size: 1524 940 787 597 519 482 459 393 362 268 3 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FspI TGC'GCA Cuts at: 2016 4840 2016 Size: 2824 3510 FspAI rTGC'GCAy Cuts at: 2016 2016 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2476 3619 5481 1678 Size: 798 1143 1862 2531 Fragments arranged by size: 2531 1862 1143 798 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2040 2072 2186 2218 3411 Size: 32 135 32 468 32 114 32 1193 Cuts at: 3411 3443 3589 3621 4975 5007 5049 5081 1373 Size: 32 146 32 1354 32 42 32 2626 Fragments arranged by size: 2626 1354 1193 468 146 135 114 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2626 3678 3924 669 Size: 988 281 688 1052 246 3079 Fragments arranged by size: 3079 1052 988 688 281 246 HindIII A'AGCT_T Cuts at: 748 2084 2862 748 Size: 1336 778 4220 Fragments arranged by size: 4220 1336 778 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 2971 3659 2971 Size: 688 5646 MfeI C'AATT_G Cuts at: 2847 3933 2847 Size: 1086 5248 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 2516 3017 10 Size: 54 1425 343 657 27 501 3327 Fragments arranged by size: 3327 1425 657 501 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2547 3144 3371 4292 4651 4810 Size: 648 1296 85 597 227 921 359 159 Cuts at: 4810 518 Size: 2042 Fragments arranged by size: 2042 1296 921 648 597 359 227 159 85 MspA1I CmG'CkG Cuts at: 1606 3750 4427 5575 5820 1606 Size: 2144 677 1148 245 2120 Fragments arranged by size: 2144 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2015 2739 2801 3692 5237 1132 Size: 97 786 724 62 891 1545 2229 Fragments arranged by size: 2229 1545 891 786 724 97 62 PasI CC'CwG_GG Cuts at: 1348 2122 3322 1348 Size: 774 1200 4360 Fragments arranged by size: 4360 1200 774 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 2570 2977 3159 1108 Size: 1234 228 407 182 4283 Fragments arranged by size: 4283 1234 407 228 182 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3002 3904 3002 Size: 902 5432 PstI C_TGCA'G Cuts at: 830 2305 3686 830 Size: 1475 1381 3478 Fragments arranged by size: 3478 1475 1381 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 SacI G_AGCT'C Cuts at: 2259 2259 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 3478 4582 6294 3478 Size: 1104 1712 3518 Fragments arranged by size: 3518 1712 1104 SfcI C'TryA_G Cuts at: 826 1045 2301 3682 4817 5498 5689 826 Size: 219 1256 1381 1135 681 191 1471 Fragments arranged by size: 1471 1381 1256 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4438 5339 5601 820 Size: 197 926 2495 901 262 1553 Fragments arranged by size: 2495 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 2015 3692 1132 Size: 883 1677 3774 Fragments arranged by size: 3774 1677 883 SspI AAT'ATT Cuts at: 5 52 2174 4258 5 Size: 47 2122 2084 2081 Fragments arranged by size: 2122 2084 2081 47 StuI AGG'CCT Cuts at: 1517 2249 1517 Size: 732 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2250 2555 3773 513 Size: 552 939 246 305 1218 3074 Fragments arranged by size: 3074 1218 939 552 305 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2928 4361 4378 4531 4716 5161 325 Size: 1255 1348 1433 17 153 185 445 1498 Fragments arranged by size: 1498 1433 1348 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2317 3349 3476 Size: 274 80 33 51 1443 340 1032 127 Cuts at: 3476 4580 6292 96 Size: 1104 1712 138 Fragments arranged by size: 1712 1443 1104 1032 340 274 138 127 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3242 3274 6294 6326 3242 Size: 32 3020 32 3250 Fragments arranged by size: 3250 3020 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3025 3025 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI