(Circular) (Six-base) MAPSORT of: pFN21AE5662.seq Check: 2396 from: 1 to: 6289 pFN21AE5662 6289 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2366 2698 2366 Size: 332 5957 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3632 1937 Size: 1695 4594 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 2930 3176 3528 4352 5736 6004 1632 Size: 872 426 246 352 824 1384 268 1917 Fragments arranged by size: 1917 1384 872 824 426 352 268 246 AfeI AGC'GCT Cuts at: 2744 2744 Size: 6289 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 2915 5188 6165 1203 Size: 1712 2273 977 1327 Fragments arranged by size: 2273 1712 1327 977 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 3504 5018 3504 Size: 1514 4775 AleI CACnn'nnGTG Cuts at: 1166 3137 1166 Size: 1971 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6289 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 2252 Size: 32 6257 AlwNI CAG_nnn'CTG Cuts at: 3022 3376 3514 4032 5604 3022 Size: 354 138 518 1572 3707 Fragments arranged by size: 3707 1572 518 354 138 ApaI G_GGCC'C Cuts at: 2327 2343 3190 3315 2327 Size: 16 847 125 5301 Fragments arranged by size: 5301 847 125 16 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2900 3062 3614 1870 Size: 73 957 162 552 4545 Fragments arranged by size: 4545 957 552 162 73 BamHI G'GATC_C Cuts at: 3619 3986 3619 Size: 367 5922 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2626 2878 3610 5065 618 Size: 325 217 627 839 252 732 1455 1842 Fragments arranged by size: 1842 1455 839 732 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2327 2343 2994 3112 3190 3315 3588 3608 Size: 936 16 651 118 78 125 273 20 Cuts at: 3608 1391 Size: 4072 Fragments arranged by size: 4072 936 651 273 125 118 78 20 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2309 2462 2918 928 Size: 971 410 153 456 4299 Fragments arranged by size: 4299 971 456 410 153 BbvCI CC'TCA_GC Cuts at: 2715 2715 Size: 6289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2852 2886 4480 4514 1903 Size: 34 50 34 831 34 1594 34 3678 Fragments arranged by size: 3678 1594 831 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3009 4900 136 Size: 107 122 71 631 605 1337 1891 1525 Fragments arranged by size: 1891 1525 1337 631 605 122 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 3706 3706 Size: 6289 Bme1580I G_kGCm'C Cuts at: 1790 2024 2327 2343 2883 2899 3190 3315 4350 Size: 234 303 16 540 16 291 125 1035 Cuts at: 4350 5506 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1035 540 303 291 234 125 16 16 BmgBI CAC'GTC Cuts at: 1318 3501 1318 Size: 2183 4106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4020 4978 Size: 416 53 83 399 24 2114 489 958 Cuts at: 4978 442 Size: 1753 Fragments arranged by size: 2114 1753 958 489 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2162 2279 2411 2753 3182 3462 3556 Size: 818 105 117 132 342 429 280 94 Cuts at: 3556 4949 1239 Size: 1393 2579 Fragments arranged by size: 2579 1393 818 429 342 280 132 117 105 94 Bpu10I CC'TnA_GC Cuts at: 2498 2715 2498 Size: 217 6072 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4414 5279 5577 4414 Size: 865 298 5126 Fragments arranged by size: 5126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4952 882 Size: 617 3453 2219 Fragments arranged by size: 3453 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2657 2918 4080 493 Size: 711 1453 261 1162 2702 Fragments arranged by size: 2702 1453 1162 711 261 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4096 4478 5113 275 Size: 53 83 186 936 2563 382 635 1451 Fragments arranged by size: 2563 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2298 3208 4722 5120 5394 5541 5952 2298 Size: 910 1514 398 274 147 411 2635 Fragments arranged by size: 2635 1514 910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 2252 2282 1178 Size: 30 165 30 627 30 192 30 5185 Fragments arranged by size: 5185 627 192 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2212 1186 Size: 844 182 5263 Fragments arranged by size: 5263 844 182 BseYI C'CCAG_C Cuts at: 2326 2398 3105 3168 3429 3490 5492 5988 2326 Size: 72 707 63 261 61 2002 496 2627 Fragments arranged by size: 2627 2002 707 496 261 72 63 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2726 3203 1163 Size: 573 339 651 477 4249 Fragments arranged by size: 4249 651 573 477 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3739 4500 5528 1540 Size: 275 186 1738 761 1028 2301 Fragments arranged by size: 2301 1738 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 2595 2833 4350 4435 5506 2294 Size: 301 238 1517 85 1071 3077 Fragments arranged by size: 3077 1517 1071 301 238 85 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 BsmBI CGTCTCn'nnnn_ Cuts at: 2079 3491 2079 Size: 1412 4877 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2294 2327 2343 2595 2833 2883 Size: 399 234 270 33 16 252 238 50 Cuts at: 2883 2899 2994 3112 3190 3315 3588 3608 4350 Size: 16 95 118 78 125 273 20 742 Cuts at: 4350 4435 5506 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 273 270 252 238 234 125 118 95 85 78 50 33 20 16 16 BspEI T'CCGG_A Cuts at: 2298 5952 2298 Size: 3654 2635 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2366 2388 2698 3644 844 Size: 1522 22 310 946 3489 Fragments arranged by size: 3489 1522 946 310 22 BsrBI CCG'CTC Cuts at: 1394 1823 3049 4176 1394 Size: 429 1226 1127 3507 Fragments arranged by size: 3507 1226 1127 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4784 4958 65 Size: 1183 3536 174 1396 Fragments arranged by size: 3536 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2649 3344 4933 5120 1700 Size: 110 839 695 1589 187 2869 Fragments arranged by size: 2869 1589 839 695 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 4349 5361 4349 Size: 1012 5277 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6289 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 3331 1184 Size: 2147 4142 BstXI CCAn_nnnn'nTGG Cuts at: 2611 3139 2611 Size: 528 5761 BstYI r'GATC_y Cuts at: 1972 3018 3619 3986 4371 4388 5840 5926 5938 Size: 1046 601 367 385 17 1452 86 12 Cuts at: 5938 6284 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1046 601 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2449 2449 Size: 6289 BtgI C'CryG_G Cuts at: 513 1065 2514 3339 513 Size: 552 1449 825 3463 Fragments arranged by size: 3463 1449 825 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2855 487 Size: 683 56 1629 3921 Fragments arranged by size: 3921 1629 683 56 BtsI GCAGTG_nn' Cuts at: 3424 3805 4599 4619 3424 Size: 381 794 20 5094 Fragments arranged by size: 5094 794 381 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2096 2855 5296 809 Size: 507 780 759 2441 1802 Fragments arranged by size: 2441 1802 780 759 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2983 3283 Size: 54 1425 50 275 18 337 816 300 Cuts at: 3283 3342 3736 4625 6254 8 Size: 59 394 889 1629 43 Fragments arranged by size: 1629 1425 889 816 394 337 300 275 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3736 1537 Size: 275 1924 4090 Fragments arranged by size: 4090 1924 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 4219 1353 Size: 836 2030 3423 Fragments arranged by size: 3423 2030 836 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2564 4876 5250 5396 387 Size: 1064 138 975 2312 374 146 1280 Fragments arranged by size: 2312 1280 1064 975 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2162 2279 2411 2504 2753 2930 Size: 393 425 105 117 132 93 249 177 Cuts at: 2930 3176 3182 3462 3528 3556 4352 4949 5736 Size: 246 6 280 66 28 796 597 787 Cuts at: 5736 6004 1239 Size: 268 1524 Fragments arranged by size: 1524 796 787 597 425 393 280 268 249 246 177 132 117 105 93 66 28 6 EcoNI CCTnn'n_nnAGG Cuts at: 3161 3161 Size: 6289 EcoO109I rG'GnC_Cy Cuts at: 2137 2323 2339 2340 2377 3187 3311 3312 2137 Size: 186 16 1 37 810 124 1 5114 Fragments arranged by size: 5114 810 186 124 37 16 1 1 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2899 2899 Size: 6289 FseI GG_CCGG'CC Cuts at: 3348 3348 Size: 6289 FspI TGC'GCA Cuts at: 2426 4795 2426 Size: 2369 3920 HaeII r_GCGC'y Cuts at: 1678 2746 5436 1678 Size: 1068 2690 2531 Fragments arranged by size: 2690 2531 1068 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2432 2464 3490 Size: 32 135 32 458 32 370 32 1026 Cuts at: 3490 3522 4930 4962 5004 5036 1373 Size: 32 1408 32 42 32 2626 Fragments arranged by size: 2626 1408 1026 458 370 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2625 5403 5587 1305 Size: 1320 2778 184 2007 Fragments arranged by size: 2778 2007 1320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 3285 10 Size: 54 1425 343 337 1116 3014 Fragments arranged by size: 3014 1425 1116 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 2945 3137 3179 3227 4247 4606 Size: 648 1443 336 192 42 48 1020 359 Cuts at: 4606 4765 518 Size: 159 2042 Fragments arranged by size: 2042 1443 1020 648 359 336 192 159 48 42 MspA1I CmG'CkG Cuts at: 1606 2059 2173 3494 3705 4382 5530 5775 1606 Size: 453 114 1321 211 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 453 245 211 114 NaeI GCC'GGC Cuts at: 3346 3346 Size: 6289 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 4288 Fragments arranged by size: 4288 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NgoMIV G'CCGG_C Cuts at: 3344 3344 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 3184 3647 5192 1132 Size: 97 1955 463 1545 2229 Fragments arranged by size: 2229 1955 1545 463 97 PasI CC'CwG_GG Cuts at: 1348 2318 1348 Size: 970 5319 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6289 PfoI T'CCnGG_A Cuts at: 2388 2529 3438 3478 3572 2388 Size: 141 909 40 94 5105 Fragments arranged by size: 5105 909 141 94 40 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2252 2284 4358 4390 5871 5903 2252 Size: 32 2074 32 1481 32 2638 Fragments arranged by size: 2638 2074 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2377 2137 Size: 240 6049 PshAI GACnn'nnGTC Cuts at: 1613 3456 1613 Size: 1843 4446 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2323 2339 3186 3311 2323 Size: 16 847 125 5301 Fragments arranged by size: 5301 847 125 16 PstI C_TGCA'G Cuts at: 830 2349 3381 3641 830 Size: 1519 1032 260 3478 Fragments arranged by size: 3478 1519 1032 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 2059 3494 2059 Size: 1435 4854 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 2069 4537 6249 2069 Size: 2468 1712 2109 Fragments arranged by size: 2468 2109 1712 SexAI A'CCwGG_T Cuts at: 2796 2796 Size: 6289 SfcI C'TryA_G Cuts at: 826 1045 2345 3377 3637 4772 5453 5644 826 Size: 219 1300 1032 260 1135 681 191 1471 Fragments arranged by size: 1471 1300 1135 1032 681 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3616 1872 Size: 1744 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4393 5294 5556 820 Size: 197 926 2450 901 262 1553 Fragments arranged by size: 2450 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2657 4080 493 Size: 2164 1423 2702 Fragments arranged by size: 2702 2164 1423 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 2207 3536 1517 Size: 690 1329 4270 Fragments arranged by size: 4270 1329 690 StyI C'CwwG_G Cuts at: 513 1065 2514 3728 513 Size: 552 1449 1214 3074 Fragments arranged by size: 3074 1449 1214 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 4316 4333 4486 4671 5116 325 Size: 1255 810 1926 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 4535 6247 Size: 274 80 33 51 1443 90 2468 1712 Cuts at: 6247 96 Size: 138 Fragments arranged by size: 2468 1712 1443 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3289 3321 3551 3583 6249 6281 3289 Size: 32 230 32 2666 32 3297 Fragments arranged by size: 3297 2666 230 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2798 1316 Size: 1482 4807 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3401 3401 Size: 6289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 3614 1870 Size: 1744 4545 XmnI GAAnn'nnTTC Cuts at: 3386 4418 3386 Size: 1032 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI