(Linear) (Six-base) MAPSORT of: KIEE5662.seq Check: 7351 from: 1 to: 1584 KIEE5662.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 361 693 1584 Size: 361 332 891 Fragments arranged by size: 891 361 332 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 925 1171 1523 1584 Size: 499 426 246 352 61 Fragments arranged by size: 499 426 352 246 61 AfeI AGC'GCT Cuts at: 0 739 1584 Size: 739 845 AflIII A'CryG_T Cuts at: 0 910 1584 Size: 910 674 AhdI GACnn_n'nnGTC Cuts at: 0 1499 1584 Size: 1499 85 AleI CACnn'nnGTG Cuts at: 0 1132 1584 Size: 1132 452 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 247 279 1584 Size: 247 32 1305 Fragments arranged by size: 1305 247 32 AlwNI CAG_nnn'CTG Cuts at: 0 1017 1371 1509 1584 Size: 1017 354 138 75 Fragments arranged by size: 1017 354 138 75 ApaI G_GGCC'C Cuts at: 0 322 338 1185 1310 1584 Size: 322 16 847 125 274 Fragments arranged by size: 847 322 274 125 16 AvaI C'yCGr_G Cuts at: 0 895 1057 1584 Size: 895 162 527 Fragments arranged by size: 895 527 162 BanI G'GyrC_C Cuts at: 0 621 873 1584 Size: 621 252 711 Fragments arranged by size: 711 621 252 BanII G_rGCy'C Cuts at: 0 322 338 989 1107 1185 1310 1583 1584 Size: 322 16 651 118 78 125 273 1 Fragments arranged by size: 651 322 273 125 118 78 16 1 BbsI GAAGACnn'nnnn_ Cuts at: 0 304 457 913 1584 Size: 304 153 456 671 Fragments arranged by size: 671 456 304 153 BbvCI CC'TCA_GC Cuts at: 0 710 1584 Size: 710 874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 847 881 1584 Size: 847 34 703 Fragments arranged by size: 847 703 34 BglI GCCn_nnn'nGGC Cuts at: 0 1004 1584 Size: 1004 580 Bme1580I G_kGCm'C Cuts at: 0 19 322 338 878 894 1185 1310 1584 Size: 19 303 16 540 16 291 125 274 Fragments arranged by size: 540 303 291 274 125 19 16 16 BmgBI CAC'GTC Cuts at: 0 1496 1584 Size: 1496 88 BmrI ACTGGGnnnn_n' Cuts at: 0 1526 1584 Size: 1526 58 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 1584 Size: 103 1481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 157 274 406 748 1177 1457 1551 Size: 52 105 117 132 342 429 280 94 Cuts at: 1551 1584 Size: 33 Fragments arranged by size: 429 342 280 132 117 105 94 52 33 Bpu10I CC'TnA_GC Cuts at: 0 493 710 1584 Size: 493 217 874 Fragments arranged by size: 874 493 217 BsaAI yAC'GTr Cuts at: 0 652 913 1584 Size: 652 261 671 Fragments arranged by size: 671 652 261 BsaWI w'CCGG_w Cuts at: 0 293 1203 1584 Size: 293 910 381 Fragments arranged by size: 910 381 293 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 55 247 277 1584 Size: 25 30 192 30 1307 Fragments arranged by size: 1307 192 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 207 1584 Size: 25 182 1377 Fragments arranged by size: 1377 182 25 BseYI C'CCAG_C Cuts at: 0 321 393 1100 1163 1424 1485 1584 Size: 321 72 707 63 261 61 99 Fragments arranged by size: 707 321 261 99 72 63 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 721 1198 1584 Size: 70 651 477 386 Fragments arranged by size: 651 477 386 70 BsiHKAI G_wGCw'C Cuts at: 0 289 590 828 1584 Size: 289 301 238 756 Fragments arranged by size: 756 301 289 238 BsmBI CGTCTCn'nnnn_ Cuts at: 0 74 1486 1584 Size: 74 1412 98 Fragments arranged by size: 1412 98 74 Bsp1286I G_dGCh'C Cuts at: 0 19 289 322 338 590 828 878 894 Size: 19 270 33 16 252 238 50 16 Cuts at: 894 989 1107 1185 1310 1583 1584 Size: 95 118 78 125 273 1 Fragments arranged by size: 273 270 252 238 125 118 95 78 50 33 19 16 16 1 BspEI T'CCGG_A Cuts at: 0 293 1584 Size: 293 1291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 361 383 693 1584 Size: 361 22 310 891 Fragments arranged by size: 891 361 310 22 BsrBI CCG'CTC Cuts at: 0 1044 1584 Size: 1044 540 BsrFI r'CCGG_y Cuts at: 0 644 1339 1584 Size: 644 695 245 Fragments arranged by size: 695 644 245 BstEII G'GTnAC_C Cuts at: 0 1326 1584 Size: 1326 258 BstXI CCAn_nnnn'nTGG Cuts at: 0 606 1134 1584 Size: 606 528 450 Fragments arranged by size: 606 528 450 BstYI r'GATC_y Cuts at: 0 1013 1584 Size: 1013 571 Bsu36I CC'TnA_GG Cuts at: 0 444 1584 Size: 444 1140 BtgI C'CryG_G Cuts at: 0 509 1334 1584 Size: 509 825 250 Fragments arranged by size: 825 509 250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 850 1584 Size: 850 734 BtsI GCAGTG_nn' Cuts at: 0 1419 1584 Size: 1419 165 DrdI GACnn_nn'nnGTC Cuts at: 0 91 850 1584 Size: 91 759 734 Fragments arranged by size: 759 734 91 EaeI y'GGCC_r Cuts at: 0 162 978 1278 1337 1584 Size: 162 816 300 59 247 Fragments arranged by size: 816 300 247 162 59 EarI CTCTTCn'nnn_ Cuts at: 0 184 1584 Size: 184 1400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 559 1584 Size: 559 1025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 157 274 406 499 748 925 1171 Size: 52 105 117 132 93 249 177 246 Cuts at: 1171 1177 1457 1523 1551 1584 Size: 6 280 66 28 33 Fragments arranged by size: 280 249 246 177 132 117 105 93 66 52 33 28 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1156 1584 Size: 1156 428 EcoO109I rG'GnC_Cy Cuts at: 0 132 318 334 335 372 1182 1306 1307 Size: 132 186 16 1 37 810 124 1 Cuts at: 1307 1584 Size: 277 Fragments arranged by size: 810 277 186 132 124 37 16 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 894 1584 Size: 894 690 FseI GG_CCGG'CC Cuts at: 0 1343 1584 Size: 1343 241 FspI TGC'GCA Cuts at: 0 421 1584 Size: 421 1163 HaeII r_GCGC'y Cuts at: 0 741 1584 Size: 741 843 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 25 57 427 459 1485 1517 1584 Size: 25 32 370 32 1026 32 67 Fragments arranged by size: 1026 370 67 32 32 32 25 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 620 1584 Size: 620 964 MscI TGG'CCA Cuts at: 0 164 1280 1584 Size: 164 1116 304 Fragments arranged by size: 1116 304 164 MslI CAynn'nnrTG Cuts at: 0 604 940 1132 1174 1222 1584 Size: 604 336 192 42 48 362 Fragments arranged by size: 604 362 336 192 48 42 MspA1I CmG'CkG Cuts at: 0 54 168 1489 1584 Size: 54 114 1321 95 Fragments arranged by size: 1321 114 95 54 NaeI GCC'GGC Cuts at: 0 1341 1584 Size: 1341 243 NcoI C'CATG_G Cuts at: 0 509 1584 Size: 509 1075 NgoMIV G'CCGG_C Cuts at: 0 1339 1584 Size: 1339 245 NspI r_CATG'y Cuts at: 0 1179 1584 Size: 1179 405 PasI CC'CwG_GG Cuts at: 0 313 1584 Size: 313 1271 PfoI T'CCnGG_A Cuts at: 0 383 524 1433 1473 1567 1584 Size: 383 141 909 40 94 17 Fragments arranged by size: 909 383 141 94 40 17 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 1584 Size: 247 32 1305 Fragments arranged by size: 1305 247 32 PpuMI rG'GwC_Cy Cuts at: 0 132 372 1584 Size: 132 240 1212 Fragments arranged by size: 1212 240 132 PshAI GACnn'nnGTC Cuts at: 0 1451 1584 Size: 1451 133 PspOMI G'GGCC_C Cuts at: 0 318 334 1181 1306 1584 Size: 318 16 847 125 278 Fragments arranged by size: 847 318 278 125 16 PstI C_TGCA'G Cuts at: 0 344 1376 1584 Size: 344 1032 208 Fragments arranged by size: 1032 344 208 PvuII CAG'CTG Cuts at: 0 54 1489 1584 Size: 54 1435 95 Fragments arranged by size: 1435 95 54 ScaI AGT'ACT Cuts at: 0 64 1584 Size: 64 1520 SexAI A'CCwGG_T Cuts at: 0 791 1584 Size: 791 793 SfcI C'TryA_G Cuts at: 0 340 1372 1584 Size: 340 1032 212 Fragments arranged by size: 1032 340 212 SnaBI TAC'GTA Cuts at: 0 652 1584 Size: 652 932 StuI AGG'CCT Cuts at: 0 202 1531 1584 Size: 202 1329 53 Fragments arranged by size: 1329 202 53 StyI C'CwwG_G Cuts at: 0 509 1584 Size: 509 1075 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 385 1584 Size: 385 1199 TatI w'GTAC_w Cuts at: 0 62 1584 Size: 62 1522 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1284 1316 1546 1578 1584 Size: 1284 32 230 32 6 Fragments arranged by size: 1284 230 32 32 6 Tth111I GACn'n_nGTC Cuts at: 0 793 1584 Size: 793 791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1396 1584 Size: 1396 188 XmnI GAAnn'nnTTC Cuts at: 0 1381 1584 Size: 1381 203 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII ScaI SexAI SfcI SnaBI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI